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Abstract

Survival of Lactobacillus paracasei. was investigated through alginate-microencapsulation followed by freezing dry and storage at 4 ℃ for 28 d or simulated gastric and intestinal digestion. Results suggested that Soy protein isolate is suitable inner wall materials, isomaltose could improve survival of Lactobacillus paracasei during encapsulation and subsequent freezing dry process. When the capsulated Lactobacillus paracasei was prepared by using 2% of alginate and 0.2 mol/L CaCl2 with 3% SPI as inner wall material, 5% isomaltose as protectant, the 82% yield of Lactobacillus paracasei microcapsules and 93.31% encapsulation efficiency were achieved. The 3 h survival of capsulated Lactobacillus paracasei in gastric digestion is around 58.97%. Incubation experiments in the pancreatin-bile solution indicated the Lactobacillus paracasei could be released basically after 45 min. Alginate-microencapsulation method is suitable to the industrialized application of lactic acid encapsulation.

Publication Date

4-28-2015

First Page

227

Last Page

231

DOI

10.13652/j.issn.1003-5788.2015.02.051

References

[1] 雷欣宇,曾凡坤,康建平,等.乳酸菌的生理功能及其食用制剂制备技术进展[J].食品与发酵科技, 2012,48(2):5~8.
[2] Dave R I, Shah N P. Viability of yoghurt and probiotic bacteria in yoghurts made from commercial starter cultures [J]. International Dairy Journal, 1997,7(1):31~41.
[3] 庄海霁,雷虹,张铁丹,等.副干酪乳杆菌的应用研究进展[J].生物技术通讯, 2006,17(6):989~991.
[4] Paulraj Kanmani R, Satish Kumar N, Yuvaraj KA, et al. Effect of cryopreservation and microencapsulation of lactic acid bacterium Enterococcus faecium MC13 for long-term storage[J]. Biochemical Engineering Journal, 2011, 58~59:140~147.
[5] Monteagudo-Mera A, Rodríguez-Aparicio L, Rúa J, et al. In vitro evaluation of physiolog ical probiotic properties of different lactic acid bacteria strains of dairy and human origin[J]. Journal of Functional Foods, 2012, 4(2):531~541.
[6] Guimaraes, RR, Vendramini ALD, dos Santos AC, et al. Development of probiotic beads similar to fish eggs[J]. Journal of Functional Foods, 2013, 5(2): 968~973.
[7] Adhikari K, Mustapha A, Grün I, et al. Viability of microencapsulated bifidobacteria in set yogurt during refrigerated storage[J]. Journal of Dairy Science, 2000, 83(9): 1 946~1 951.
[8] 熊涛,冯超,谢明勇,等.植物乳杆菌NCU116微胶囊制备工艺的优化设计[J].食品科学,2012,33(1):152~156.
[9] Chandramouli V, Kailasapathy K, Peiris P, et al. An improved method of microencapsulation and its evaluation to protect Lactobacillus spp in simulated gastric conditions[J]. Journal of Microbiological Methods, 2004,56(1):27~35.
[10] Han B, Bischof J C. Direct cell injury associated with eutectic crystallization during freezing[J].Cryobiology, 2004,48(1): 8~21.
[11] van de Guchte M, Serror P, Chervaux C, et al. Stress responses in lactic acid bacteria[J]. Antonie Van Leeuwenhoek, 2002,82(1~4):187~216.
[12] 朱琳,刘宁,张英华,等.乳酸菌细胞膜的冻干损伤[J].食品科学,2006,27(2):266~269.

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