In order to determine the optimal conditions for the extraction of polyphenols from Dendrobium huoshanense by ultrasonic-assisted cellulase, response surface methodology was used to optimize the extraction process. Antioxidant activity of Dendrobium huoshanense polyphenols was evaluated. The results showed that the optimal conditions for the extraction of polyphenols were as follows: ultrasonic power 180 W, ultrasonic time 20 min, cellulase concentra-tion 2.1 mg/mL, enzymolysis temperature 57 ℃, enzymolysis time 71 min, and enzymolysis pH 5. Under these conditions, the average yield of polyphenols was 13.74 mg/g. Antioxidant assays revealed that Dendrobium huoshanense polyphenols had significant antioxidant activity. DPPH and ABTS radical scavenging activities had a stronger relationship with the content of polyphenols, and their hemi-inhibitory concentrations (IC50) were 0.057 and 0.027 mg/mL, respectively.

Publication Date


First Page


Last Page





[1] 包雪声, 顺庆生, 张申洪. 中国药用石斛图志[M]. 上海: 上海科学技术文献出版社, 2005: 32.
[2] WU Chin-tung, HUANG Keng-shiang, YANG Chih-hui, et al. Inhibitory effects of cultured Dendrobium tosaense on atopic dermatitis murine model[J]. International Journal of Pharmaceutics, 2014, 463(2): 193-200.
[3] 黄琴, 沈杨霞, 张成静, 等. 铁皮石斛多酚和黄酮含量及与抗氧化活性的相关性[J]. 应用与环境生物学报, 2014, 20(3): 438-442 .
[4] LUO Jian-ping, DENG Yuan-yuan, ZHA Xue-qiang. Mechanism of polysaccharides from Dendrobium huoshanense on streptozotocin-induced diabetic cataract[J]. Pharmaceutical Biology, 2008, 46(4): 24-249.
[5] XING Xiao-hui, STEVE W C, NIE Shao-ping, et al. A review of isolation process, structural characteristics, and bioactivities of water-soluble polysaccharides from Dendrobium plants[J]. Bioactive Carbohydrates and Dietary Fibre, 2013, 1(2): 131-147.
[6] ZHA Xue-qiang, LI Xiao-long, ZHANG Hai-lin, et al. Pectinase hydrolysis of Dendrobium huoshanense polysaccharide and its effect on protein nonenzymatic glycation[J]. International Journal of Biological Macromolecules, 2013, 61(10): 439-447.
[7] 尹志娜. 植物多酚分离提取方法和生物功能研究进展[J]. 生命科学仪器, 2010, 8(7): 43-49.
[8] 王海燕, 崔春, 赵谋明, 等. 烟草多酚提取工艺优化及成分定性分析[J]. 华南理工大学学报: 自然科学版, 2008, 36(3): 64-68.
[9] 李俶, 沈佩仪, 吴华星, 等. 超声波提取菠萝皮渣中多酚类物质的研究[J]. 食品与机械, 2011, 27(2): 55-58.
[10] LUENGO E, LVAREZ I, RASO J. Improving the pressing extraction of polyphenols of orange peel by pulsed electric fields[J]. Innovative Food Science & Emerging Technologies, 2013, 17: 79-84.
[11] WANG Ru-feng, CHEN Ping, JIA Fang, et al. Optimization of polysaccharides from Panax japonicus C.A. Meyer by RSM and its anti-oxidant activity[J]. International Journal of Biological Macromolecules, 2012, 50(2): 331-336.
[12] WANG Zan-yong, WANG Chen-yu, QUAN Yue. Extraction of polysaccharides from Phellinus nigricans mycelia and their antioxidant activities in vitro[J]. Carbohydrate Polymers, 2014, 99(1): 110-115.
[13] 陈晨, 胡文忠, 田沛源, 等. 超声辅助提取香蕉皮多酚工艺优化及抗氧化分析[J]. 食品科学, 2014, 35(2): 12-17.
[14] 杜若源, 谢晶, 王婷, 等. 超声波辅助提取银杏叶总黄酮的工艺优化[J]. 食品与机械, 2015, 31(1): 167-170.
[15] 李俶, 沈佩仪, 吴华星, 等. 超声波提取菠萝皮渣中多酚类物质的研究[J]. 食品与机械, 2011, 27(2): 55-58.
[16] 包怡红, 王硕, 王文琼, 等. 超声波酶法提取红松树皮中多酚类化合物的研究[J]. 食品工业科技, 2013, 34(3): 232-236.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.