Objective:This study focused on exploring thein vitrolipid-lowering efficacy of Puerarin lobata extract.Methods: The adsorption ratios of oil, cholesterol, sodium glycocholate and sodium taurocholate were evaluated by a multi index comprehensive scoring method to optimize the P. lobata alcohol extraction process using Box-Behnken response surface methodology with extraction time, extraction times and ethanol concentration as influencing factors.In vitro lipid-lowering activity was further validated by comparing the levels of TG and TC accumulation and the extent of intracellular lipid droplet accumulation in a model of human hepatoma (HepG2) fatty liver cells induced by palmitic acid (PA).Results: The optimal alcohol extraction process of P. lobata was 70% by volume of ethanol, 1 h of extraction time and 3 times of extraction time;P. lobata extract could obviously reduce the contents of TG and TC in cells and effectively reduce the intracellular lipid droplet aggregation.Conclusion: The optimized P. lobata extraction process was stable and reliable, manipulable, and showed good lipid-lowering activity in vitro.

Publication Date


First Page


Last Page





[1] 龚受基,蒙彦妃,曹惠怡,等.凡纳滨对虾蛋白体外降脂作用分析[J].食品工业科技,2020,41(11):316-321.LONG S J,MENG Y F,CAO H Y,et al.Hypolipidemic effects of protein from Penaeus vannamei in vitro[J].Science and Technology of Food Industry,2020,41(11):316-321.
[2] 杜令娟,康宁,李德海,等.粗毛纤孔菌三萜初级纯化及体外降血脂研究[J].森林工程,2017,33(5):29-34.DU L J,KANG N,LI D H,et al.Primary purification and in vitro hypolipidemic activity of triterpenoid from Inonotus hispidus[J].Forest Engineering,2017,33(5):29-34.
[3] 任正肖,车萍,李紫薇,等.葛根素药理作用的研究进展[J].山东化工,2019,48(19):74-75.REN Z X,CHE P,LI Z W,et al.Research progress on pharmacological action ofpuerarin[J].Shandong Chem Ind,2019,48(19):74-75.
[4] 魏述永.葛根及葛根素脑保护作用的研究进展[J].食品科学,2015,36(17):259-263.WEI S Y.Progress in cerebral protection of Pueraria lobata(Willd.)Ohwi(Fabaceae)and puerarin[J].Food Science,2015,36(17):259-263.
[5] WANG S,YANG F J,SHANG L C,et al.Puerarin protects against high-fat high-sucrose diet-induced non-alcoholic fatty liver disease by modulating PARP-1/PI3K/AKTsignaling pathway and facilitating mitochondrial homeostasis[J].Phytother Res,2019,33(9):2 347-2 359.
[6] 王苗,张红印,范琳,等.葛根蛋白提取工艺及其体外抗氧化性研究[J].食品研究与开发,2021,42(1):73-79.WANG M,ZHANG H Y,FAN L,et al.Study on extraction technology of Pueraria lobataprotein and its antioxidant activity in vitro[J].Food Research and Development,2021,42(1):73-79.
[7] 罗秋水,杜华英,熊建华,等.葛根异黄酮类化合物提取工艺优化及其抗氧化活性研究[J].中国食品学报,2015,15(2):104-110.LUO Q S,DU H Y,XIONG J H,et al.Extraction process optimization ofpueraria isoflavones and studies on its antioxidant activity[J].Journal of Chinese Institute of Food Science and Technology,2015,15(2):104-110.
[8] SANGNARK A,NOOMHORM A.Effect of particle sizes on functional properties of dietary fibre prepared from sugarcane bagasse[J].Food Chem,2003,80(2):221-229.
[9] 钟希琼,胡文娥,林丽超.膳食纤维对油脂、胆固醇、胆酸钠和亚硝酸根离子吸附作用的研究[J].食品工业科技,2010,31(5):134-136.ZHONG X Q,HU W E,LIN L C.Study on the adsorption of dietary fiber for oil and fat,cholesterol,sodium cholate and NO-2[J].Science and Technology of Food Industry,2010,31(5):134-136.
[10] 孙凯峰,包怡红.微生物发酵对黑木耳总糖含量和体外调脂活性的影响[J].中草药,2018,49(16):3 781-3 787.SUN K F,BAO Y H.Effect of microbial fermentation on total sugar content and in vitro hypolipidemic effect of Auricularia auricula[J].Chinese Traditional and Herbal Drugs,2018,49(16):3 781-3 787.
[11] 邓雪,黄惠华.茶花水溶性蛋白的分离纯化及其体外吸附胆酸盐能力的研究[J].现代食品科技,2013,29(1):63-67.DENG X,HUANG H H.In vitro binding of bile salts by water-soluble protein extract from tea flower[J].Modern Food Science and Technology,2013,29(1):63-67.
[12] 钱雅雯,魏佳,张政,等.籽瓜多糖提取工艺的响应面优化及其体外降血脂活性[J].食品工业科技,2020,41(2):101-107.QIAN Y W,WEI J,ZHANG Z,et al.Optimization of polysaccharides extraction from seed melon by response surface methodology and its hypolipidemic effects in vitro[J].Science and Technology of Food Industry,2020,41(2):101-107.
[13] WINBERG M E,MOTLAGH M K,STENKULA K G,et al.Adiponutrin:A multimeric plasma protein[J].Biochemical and Biophysical Research Communications,2014,446(4):1 114-1 119.
[14] 陶嘉,唐超,孟凡强,等.黑木耳多肽对棕榈酸诱导的脂肪肝细胞的降脂作用[J].食品科学,2022,43(3):106-113.TAO J,TANG C,MENG F Q,et al.Lipid-lowering effects of Auricularia auriculapeptides on palmitic acid-induced non-alcoholic fatty acid liver[J].Food Science,2022,43(3):106-113.
[15] 陈龙云,刘笑玎,师晓华,等.油红O染色法在2D和3D细胞培养原代胰腺星形细胞鉴定中的应用[J].诊断病理学杂志,2020,27(12):877-880.CHEN L Y,LIU X D,SHI X H,et al.Application of oil red O staining in identification of primary pancreatic stellate cells cultured in 2D and 3D medium[J].Chinese Journal of Diagnostic Pathology,2020,27(12):877-880.
[16] 李少华,高愿军,李翠翠,等.微波辅助酶法提取葛根黄酮的工艺优化及抗氧化活性研究[J].食品安全质量检测学报,2022,13(1):217-222.LI S H,GAO Y J,LI C C,et al.Optimization of extraction process of Radix puerariae flavonoids by microwave-assisted enzymatic method and study on its antioxidant activity[J].Journal of Food Safety and Quality,2022,13(1):217-222.
[17] 于文杰,赵宁,张筠.响应面法测定葛根中葛根素的提取工艺[J].粮食与油脂,2021,34(12):108-111,116.YU W J,ZHAO N,ZHANG J.Determination the extraction process of puerarin from pueraria iobata by response surface methodology[J].Cereals & Oils,2021,34(12):108-111,116.
[18] 颜飞翔,朱丹,牛广财,等.秋香梨果醋醋酸发酵工艺优化及抗氧化活性研究[J].食品与机械,2021,37(4):184-188,225.YAN F X,ZHU D,NIU G C,et al.Optimization of acetic acid fermentation process and antioxidant activity of Qiuxiang pear vinegar[J].Food & Machinery,2021,37(4):184-188,225.
[19] FUNGWE T V,CAGEN L M,COOK G A,et al.Dietary cholesterol stimulates hepatic biosynthesis of triglyceride and reduces oxidation of fatty acids in the rat[J].Journal of Lipid Research,1993,34(6):933-941.
[20] IBRAHIM A,SHAFIE N H,MOHD ESA N,et al.Mikania micrantha extract inhibits HMG-CoA reductase and ACAT2 and ameliorates hypercholesterolemia and lipid peroxidation in high cholesterol-fed rats[J].Nutrients,2020,12(10):3 077.

Included in

Food Science Commons



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.