Objective:To study and optimize the extraction technology of bacteriostatic extract from Indocalamus leaves. Methods:The extracts were drawn from Indocalamus leaves by ultrasound-assisted, refluxing and immersing method with extraction solvents: water, ethyl acetate, pH 4 of hydrochloric acid solution, pH 4 of acetic acid solution, pH 10 of ammonia solution and different volume ratio ethyl alcohol. The tests were carried out to analyze the antimicrobial effects with indicator bacteria Bacillus subtilis, Pseudomonas aeruginosa, Shigella Dysenteriae and Salmonella typhi and the total bacteriostatic circle was as inspection indicator, to find out the best extraction solvent and the best extraction method. Using water as extraction solvent, ultrasonic extraction method as method, an orthogonal test L9(33) was carried out by adjusting three impact factors. The impact factors were liquid-solid ratio, sonication period and sonication frequency. The orthogonal test found the optimal extraction process parameters and obtained the best extract. At the same time, the antimicrobial effect of the extract obtained under the optimized conditions was investigated according to the standard series of reference substances. Results:The results showed that water was the best solvent, and ultrasound-assisted method was the best method. The best extraction process parameters were as follows: the ratio of liquid to solid was 16∶1(mL/g), ultrasonic frequency was 4 times, ultrasonic period was 55 minutes. The inhibitory effect of the best extract on Bacillus Subtilis and Salmonella Typhi was equivalent to the effect of 0.01 and 0.03 mg/mL gentamicin, respectively. The bacteriostatic effects on Pseudomonas Aeruginosa and Shigella Dysenteriae were greater than that of 0.05 mg/mL gentamicin. Conclusion:The water ultrasonic method is an effective method for extracting antibacterial substances from Indocalamus leaves, and the inhibitive effect of the extract on Bacillus Subtilis,Pseudo monas Aeruginosa,Shigella Dysenteriae and Salmonella Typhi is remarkable.

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[1] 刘立萍,沈宏桂,罗宏泉,等.返青箬叶中铜和锌在不同食品模拟物中迁移情况[J].包装工程,2021,42(21):72-78.LIU L P,SHEN H G,LUO H Q,et al.Study on the migration of copper and zinc in regreen Indocalamus leaves in different food stimulants[J].Packaging Engineering,2021,42(21):72-78.
[2] 刘立萍,陈慧,罗宏泉,等.怀化市食品包装用箬叶所属物种调查和7种元素含量测定[J].包装工程,2022,43(5):122-129.LIU L P,CHEN H,LUO H Q,et al.Species survey and seven elements determination of Indocalamus leaves for food packaging in Huaihua area[J].Packaging Engineering,2022,43(5):122-129.
[3] 陈云辉.粽子的蒸煮等加工特性分析及其工艺优化[D].广州:华南理工大学,2013:5.CHEN Y H.Propertiesanalysis and optimization of cooking process of Zongzi[D].Guangzhou:South China University of Technology,2013:5
[4] 龚乃超,锁进猛,张浩,等.半仿生法提取箬叶总黄酮的研究[J].绿色科技,2018,6(3):178-181.GONG N C,SHUO J M,ZHANG H,et al.Extraction of total flavonoids from Indocalamus leaves by semi-bionic method[J].Journal of Green Science and Technology,2018,6(3):178-181.
[5] 李冬林,金雅琴,王文君,等.箬竹叶片营养成分分析[J].江苏林业科技,2021,48(1):41-45.LI D L,JIN Y Q,WANG W J,et al.Analysis of nutrient components of Indocalamus tessellatus leaves[J].Journal of Jiangsu Forestry Science & Technology,2021,48(1):41-45.
[6] 陈召桂,何剑飞,成凌,等.微波消解-AAS法测定箬叶中微量离子的含量[J].食品研究与开发,2014,35(8):76-78.CHEN Z G,HE J F,CHENG L,et al.Determination of trace elements in Indocalamus leaves by microwave digestion-atomic absorption apectroscopy[J].Food Research and Development,2014,35(8):76-78.
[7] SHEN M,CHEN L Y,HAN W L,et al.Methods for the determination of heavy metals in Indocalamus leaves after differentpreservation treatment using inductively-coupled plasma mass spectrometry[J].Ournal Microchemical Journal,2018,139:295-300.
[8] 李水芳,文瑞芝,曾栋,等.气相色谱—质谱法分析湖南产阔叶箬竹叶挥发油的化学成分[J].质谱学报,2007,28(2):117-121.LI S F,WEN R Z,ZHONG D.et al.Analysis of essential oils from Indocalamus latifolius leaves by GC/MS[J].Journal of Chinese Mass Spectrometry Society,2007,28(2):117-121.
[9] 乐薇,吴士筠.热浸法提取箬叶总黄酮的动力学研究[J].江苏农业科学,2015,43(11):359-361.LE W,WU S J.Kinetic study on the extraction of total flavones from Indocalamus leaves by hot dipping method[J].Jiangsu Agricultural Science,2015,43(11):359-361.
[10] 乐薇,赵淳,韦琴.聚酰胺树脂对箬叶黄酮碳苷分离效果的研究[J].保鲜与加工,2018,18(6):100-106.YUE W,ZHAO C,WEI Q.The separation of c-glycosylflavones from Indocalamus leaf by polyamide resin[J].Storage and Process,2018,18(6):100-106.
[11] 闫荣玲,廖阳,唐秋玲,等.粽叶中黄酮的提取工艺及其与稀土离子在沸水中的析出规律[J].天然产物研究与开发,2016(28):1 313-1 318.YAN R L,LIAO Y,TANG Q L,et al.Extraction process of flavonoids and precipitation law of flavonoidsand rare earth elements in boiling water from Indocalamus latifolius leaves[J].Natural Products Research Development,2016(28):1 313-1 318.
[12] 孙克奎.响应面法优化箬叶多糖提取工艺及抑菌活性研究[J].食品工业,2014,35(4):35-38.SUN K K.Optimization of extraction of polysaccharide from Indocalamus tessellatus leaves and its antibacteri activity[J].Food Industry,2014,35(4):35-38.
[13] 李俊超,胡坚,杨春雷,等.箬竹叶提取物对烟草病原真菌的抑制作用[J].化学与生物工程,2015,32(2):43-47.LI J C,HU J,YANG C L,et al.Hibitory activity of Indocalamus tessellatus leaves against pathogenic fungi of tobacco[J].Chemistry &Bioengineering,2015,32(2):43-47.
[14] 乐薇,佘雨珊,吴士筠.提取方法对箬叶总黄酮抑菌效果的影响[J].化学与生物工程,2015,32(11):31-33.YUE W,SHE Y S,WU S J.Effect of extraction methods on antibacterial activities of total flavonoids from Indocalamus leaves[J].Chemistry & Bioengineering,2015,32(11):31-33.
[15] 吉瑞冬,孙雯,乐薇,等.箬叶黄酮抑菌洗手液的研制[J].化工技术与开发,2017,46(1):1-4.JI R D,SUN W,YUE W,et al.Research on antibacterial hand sanitizer with Indocalamus leaf[J].Technology & Development of Chemical Industry,2017,46(1):1-4.
[16] 何舟,陈新,杨桃,等.箬叶中抑菌成分的提取及抑菌试验[J].武汉工业学院学报,2013,32(1):35-39.HE Z,CHEN X,YANG T,et al.Research on extraction of antibacterial active components from aspidistra elatior blume and study on its inhibitory activity[J].Journal of Wuhan Polytechnic University,2013,32(1):35-39.
[17] 邓龙兴,余君,覃光炯,等.箬竹叶提取物抗真菌活性研究[J].化学与生物工程,2019,36(7):19-21.DENG L X,YU J,QING G J,et al.Antifungal activity of extracts from Indocalamus tessellatus leaves[J].Chemistry & Bioengineering,2019,36(7):19-21.
[18] SUN J,XUN H,YU J,et al.Chemical constituents and antibacterial properties of Indocalamus latifolius mcclure leaves,the packaging material for "Zongzi"[J].Molecules,2015,20(9):15 686-15 700.
[19] NI Q X,ZHANG Y Z,XU G Z,et al.Influence of harvest season and drying method on the antioxidant activity and active compounds of two bamboo grass leaves[J].Journal of Food Processing and Preservation,2014,38(4):1 565-1 576.
[20] 刘立萍,王宁,雷小红,等.食品包装用箬叶提取物抑菌活性的初步研究[J].食品科技,2022,47(6):257-262.LIU L P,WANG N,LEI X H,et al.Primary study on the antimicrobial activity of the extracts from Indocalamus tessellatus leaves for food packaging[J].Food Science and Technology,2022,47(6):257-262.
[21] 周芳,熊海涛,张江,等.牛津杯法测定抗菌肽对四种有害微生物的抑制效果[J].饲料工业,2018,39(6):48-51.ZHOU F,XIONG H T,ZHANG J,et al.The inhibitory effect of antimicrobial peptide against four kinds of harmful microorganisms by the Oxford cup method[J].Feed Industry,2018,39(6):48-51.

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