Objective:This study was to explore the improvement of structural and emulsifying properties of rice protein by interacted with the dark blue pigment of Vaccinium bracteatum Thunb leaves. Methods:The dark blue pigment was prepared from VBTL, and the changes of structural and emulsifying properties of rice protein before and after dyeing were analyzed. Results:The formation of binary complexes between the dark blue pigment and the rice protein decreased the color values of L* and b*, and the hue of the pigment-protein complex changed to dark blue color with the increase of the pigment concentration. The water solubility of the pigment-protein complex increased, and the oil-holding capacity decreased significantly. The analysis circular dichroism spectroscopy showed the changes in the secondary structure of pigment-protein complex with a decreasing in the relative content of α-helices and β-folds and an increasing in the relative content of β-turns and random coil. At the concentration of pigment below 1.5%, foam stability increased, while foaming capacity, emulsifying capacity and emulsion stability decreased. When the concentration of pigment was higher than 1.5%, foam stability decreased, while foaming capacity, emulsifying capacity and emulsion stability increased. Conclusion:The dark blue pigment can improve the emulsifying properties of rice protein by changing its secondary structure.

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[1] 王立,练伟佳,李言,等.乌饭树资源开发利用研究进展[J].中草药,2018,49(17):4 197-4 204.WANG L,LIAN W J,LI Y,et al.Study progress of exploitation and utilization of Vaccinium bracteatum resources[J].Chinese Traditional and Herbal Drugs,2018,49(17):4 197-4 204.
[2] WANG L,ZHANG X T,ZHANG H Y,et al.Effect of Vaccinium bracteatum Thunb.leaves extract on blood glucose and plasma lipid levels in streptozotocin-induced diabetic mice[J].Journal of Ethnopharmacology,2010,130(3):465-469.
[3] 屈晶,陈霞,牛长山,等.南烛化学成分研究[J].中国中药杂志,2014,39(4):684-688.QU J,CHEN X,NIU C S,et al.Chemical constituents from Vaccinium bracteatum[J].China Journal of Chinese Materia Medica,2014,39(4):684-688.
[4] 顾文秀,谢为明,夏文水,等.超声波提取乌饭树叶色素及其稳定性的研究[J].林产化学与工业,2005,25(4):74-78.GU W X,XIE W M,XIA W S,et al.Ultrasonic extraction of pigment from leaves of Vaccinium bracteatum Thunb[J].Chemistry and Industry of Forest Products,2005,25(4):74-78.
[5] 胡志杰,蔡智慧.微波辅射提取乌饭树叶黑色素工艺的研究[J].中国野生植物资源,2007,26(6):52-54,57.HU Z J,CAI Z H.Study on extraction technology of black pigment from Vaccinium bracteatum Thunb.leaf under microwave radiation[J].Chinese Wild Plant Resources,2007,26(6):52-54,57.
[6] 王立,蒋甜燕,张晖,等.酶法提取乌饭树树叶黑色素及其稳定性研究[J].食品工业科技,2008(10):224-226.WANG L,JIANG T Y,ZHANG H,et al.Study on extraction of black pigment from Vaccinium bracteatum Thunb.leaves by enzyme and its stability[J].Science and Technology of Food Industry,2008(10):224-226.
[7] 周兆祥,郭维华,王伯钱.乌饭树色素的研究[J].食品与发酵工业,1989(6):54-59.ZHOU Z X,GUO W H,WANG B Q.Study on the pigment of Vaccinium bracteatum[J].Food and Fermentation Industries,1989(6):54-59.
[8] 余清.乌饭树叶中黄酮等有效成分分析及抗肿瘤作用研究[D].福州:福建农林大学,2008:2-9.YU Q.Studies on flavonoids effective components in Vaccinium bracteatum Thunb.leaves and function of anti-tumor[D].Fuzhou:Fujian Agriculture and Forestry University,2008:2-9.
[9] FAN M C,FAN Y H,HUANG W P,et al.Tentative characterization of precursor compounds and co-factors of pigment formation in production of 'Wu mi' from Vaccinium bracteatum Thunb.leaves[J].Food Chemistry,2018,262:199-205.
[10] LIAN W J,FAN M C,LI T T,et al.A novel green synthesis approach for natural bluish-violet pigments derived from water extracts of Vaccinium bracteatum Thunb.leaves[J].Industrial Crops and Products,2019,142:111862.
[11] FAN M C,LIAN W J,LI T T,et al.Characterization of promising natural blue pigment from Vaccinium bracteatum Thunb.leaves:Insights of the stability and the inhibition of α-amylase[J].Food Chemistry,2020,326:126962.
[12] HU Z Y,QIU L,SUN Y,et al.Improvement of the solubility and emulsifying properties of rice bran protein by phosphorylation with sodium trimetaphosphate[J].Food Hydrocolloids,2019,96:288-299.
[13] AMAGLIANI L,OʼREGAN J,KELLY A L,et al.The composition,extraction,functionality and applications of rice proteins:A review[J].Trends in Food Science & Technology,2017,64:1-12.
[14] SEGURA-CARRETERO A,IVANOVI M,ARRÁEZ-ROMÁN D,et al.Enhanced and green extraction of bioactive compounds from Lippia citriodora by tailor-made natural deep eutectic solvents[J].Food Research International,2018,111:67-76.
[15] 刘媚琪,邱紫莹,王冬萍,等.女贞子中5种环烯醚萜苷类成分的降解动力学研究[J].天津中医药大学学报,2022,41(2):225-231.LIU M Q,QIU Z Y,WANG D P,et al.Degradation kinetics of five iridoid glycosides in Ligustrum lucidum[J].Journal of Tianjin University of Traditional Chinese Medicine,2022,41(2):225-231.
[16] 高瑾,TARIRO-UPENYU M,李志豪,等.丙二醛氧化对籽瓜种仁蛋白质结构及功能特性的影响[J].食品与机械,2019,35(11):17-21.GAO J,TARIRO-UPENYU M,LI Z H,et al.Effects of malondialdehyde oxidation on structure and functional properties of seeds-watermelon seed protein[J].Food & Machinery,2019,35(11):17-21.
[17] 孙小斐,乔玉辉.不同因素对白果蛋白持油性、持水性和起泡性的影响[J].食品与机械,2012,28(3):17-20.SUN X F,QIAO Y H.Effect of different factors on oil-absorbing,water-holding and foaming capacities of ginkgo protein[J].Food & Machinery,2012,28(3):17-20.
[18] SAAR-REISMAA P,KOEL M,TARTO R,et al.Extraction of bioactive compounds from Dipsacus fullonum leaves using deep eutectic solvents[J].Journal of Chromatography A,2022,1 677:463330.
[19] WANG L,XU Y,ZHOU S M,et al.Interaction between Vaccinium bracteatum Thunb.leaf pigment and rice proteins[J].Food Chemistry,2016,194:272-278.
[20] 潘敏尧.大米浓缩蛋白的研制及性质改性究[D].杭州:浙江大学,2006:46-52.PAN M Y.Studies on extraction of rice protein concentration and modification of rice protein[D].Hangzhou:Zhejiang University,2006:46-52.
[21] 李维瑶,何志勇,熊幼翎,等.温度对于大豆分离蛋白起泡性的影响研究[J].食品工业科技,2010,31(2):86-88.LI W Y,HE Z Y,XIONG Y L,et al.Effect of temperature on the foaming properties of bean protein isolate[J].Science and Technology of Food Industry,2010,31(2):86-88.
[22] 张凯.大米蛋白提取工艺优化、改性及理化性质的研究[D].长沙:湖南农业大学,2010:46-49.ZHANG K.Study on extraction,modification of rice protein and its physical and chemical properties[D].Changsha:Hunan Agricultural University,2010:46-49.

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