The structural characteristics and physiological activities of reduced glutathione (GSH), as well as its metabolic pathways in vivo were reviewed, and the research progress in preparation, extraction, purification, application and industrial production of GSH were summarized. Moreover, the future research directions in functional activity, industrial production and product application of GSH were prospected.

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[1] FLOHE L.Glutathione[M].Florida:CRC Press,2018.
[2] HOMMA T,FUJII J.Application of glutathione as anti-oxidative and anti-aging drugs[J].Current Drug Metabolism,2015,16(7):560-571.
[3] ADEOYE O,OLAWUMI J,OPEYEMI A,et al.Review on the role of glutathione on oxidative stress and infertility[J].JBRA Assisted Reproduction,2018,22(1):61-66.
[4] GAUCHER C,BOUDIER A,BONETTI J,et al.Glutathione:Antioxidant properties dedicated to nanotechnologies[J].Antioxidants,2018,7(5):62-82.
[5] AHMAD M,ANJUM N A,ASIF A,et al.Real-time monitoring of glutathione in living cells using genetically encoded FRET-based ratiometric nanosensor[J].Scientific Reports,2020,10(1):1-9.
[6] RAJ RAI S,BHATTACHARYYA C,SARKAR A,et al.Glutathione:Role in oxidative/nitrosative stress,antioxidant defense,and treatments[J].Chemistry Select,2021,6(18):4 566-4 590.
[7] PIZZORNO J.Glutathione! [J].Integrative Medicine,2014,13(1):8-12.
[8] TSIASIOTI A,TZANAVARAS P D.Determination of glutathione and glutathione disulfide using zone fluidics and fluorimetric detection[J].Talanta,2021,222:121559.
[9] ERKEKOGLU P,KOCER-GUMUSEL B.Glutathione in health and disease[M].London:Intech Open,2018:4-8.
[10] LUSHCHAK V I.Glutathione homeostasis and functions:Potential targets for medical interventions[J].Journal of Amino Acids,2012,2 012:736837.
[11] VAIRETTI M,Di PASQUA L G,CAGNA M,et al.Changes in glutathione content in liver diseases:An update[J].Antioxidants,2021,10(3):364-402.
[12] KURTZMAN C P,FELL J W,BOEKHOUT T.The yeasts,a taxonomic study[M].Amsterdam:Elsevier,2011:21-44.
[13] MINICH D M,BROWN B I.A review of dietary(phyto)nutrients for glutathione support[J].Nutrients,2019,11(9):2 073-2 092.
[14] LI Y,WEI G Y,CHEN J.Glutathione:A review on biotechnological production[J].Applied Microbiology and Biotechnology,2004,66(3):233-242.
[15] CHEN J L,XIE L,CAI J J,et al.Enzymatic synthesis of glutathione using engineered Saccharomyces cerevisiae[J].Biotechnology Letters,2013,35(8):1 259-1 264.
[16] LI W,LI Z M,YE Q.Enzymatic synthesis of glutathione using yeast cells in two-stage reaction[J].Bioprocess and Biosystems Engineering,2010,33(6):675-682.
[17] HUANG C,YIN Z M.Highly efficient synthesis of glutathione via a genetic engineering enzymatic method coupled with yeast ATP generation[J].Catalysts,2019,10(1):33-45.
[18] JIANG Y,TAO R S,SHEN Z Q,et al.Enzymatic production of glutathione by bifunctional γ-glutamylcysteine synthetase/glutathione synthetase coupled with in vitro acetate kinase-based ATP generation[J].Applied Biochemistry and Biotechnology,2016,180(7):1 446-1 455.
[19] ZHANG X,WU H,HUANG B,et al.One-pot synthesis of glutathione by a two-enzyme cascade using a thermophilic ATP regeneration system[J].Journal of Biotechnology,2017,241:163-169.
[20] CAO H,LI C C,ZHAO J,et al.Enzymatic production of glutathione coupling with an ATP regeneration system based on polyphosphate kinase[J].Applied Biochemistry and Biotechnology,2018,185(2):385-395.
[21] 张星,崔向伟,李宗霖,等.基于能量循环再生系统酶法生产谷胱甘肽[J].华东理工大学学报(自然科学版),2020,46(5):688-693.ZHANG X,CUI X W,LI Z L,et al.Enzymatic synthesis of glutathione based on energy regeneration system[J].Journal of East China University of Science and Technology,2020,46(5):688-693.
[22] CUI X W,LI Z M.High production of glutathione by in vitro enzymatic cascade after thermostability enhancement[J].AICHE Journal,2021,67(1):e17055.
[23] LIU W,ZHU X C,LIAN J Z,et al.Efficient production of glutathione with multi-pathway engineering in Corynebacterium glutamicum[J].Journal of Industrial Microbiology and Biotechnology,2019,46(12):1 685-1 695.
[24] SCHMACHT M,LORENZ E,SENZ M.Microbial production of glutathione[J].World Journal of Microbiology and Biotechnology,2017,33(6):1-13.
[25] BADR H,EL-BAZ A,MOHAMED I,et al.Bioprocess optimiza-tion of glutathione production by Saccharomyces boulardii:Biochemical characterization of glutathione peroxidase[J].Archives of Microbiology,2021,203(10):6 183-6 196.
[26] PRATYOOSH S.Frontier discoveries and innovations in interdisciplinary microbiology[M].New Delhi:Springer India,2016:1-8.
[27] JUNIOR W J F L,BINATI R L,BERSANI N,et al.Investigating the glutathione accumulation by non-conventional wine yeasts in optimized growth conditions and multi-starter fermentations[J].LWT-Food Science and Technology,2021,142:110990.
[28] AL-MADBOLY L A,KHEDR E G,ALI S M.Optimization of reduced glutathione production by a Lactobacillus plantarum isolate using Plackett-Burman and Box-Behnken designs[J].Frontiers in Microbiology,2017,8:772-780.
[29] SANTOS L O,SILVA P G P,LEMOS JUNIOR W J F,et al.Glutathione production by Saccharomyces cerevisiae:Current state and perspectives[J].Applied Microbiology and Biotechnology,2022,106:1 879-1 894.
[30] WANG C,ZHANG J,WU H,et al.Heterologous gshF gene expression in various vector systems in Escherichia coli for enhanced glutathione production[J].Journal of Biotechnology,2015,214:63-68.
[31] ZHANG J,QUAN C,WANG C,et al.Systematic manipulation of glutathione metabolism in Escherichia coli for improved glutathione production[J].Microbial Cell Factories,2016,15(1):1-12.
[32] WANG D Z,WANG C,WU H,et al.Glutathione production by recombinant Escherichia coli expressing bifunctional glutathione synthetase[J].Journal of Industrial Microbiology and Biotechnology,2016,43(1):45-53.
[33] HARA K Y,AOKI N,KOBAYASHI J,et al.Improvement of oxidized glutathione fermentation by thiol redox metabolism engineering in Saccharomyces cerevisiae[J].Applied Microbiology and Biotechnology,2015,99(22):9 771-9 778.
[34] KRESNOWATI M T A P,IKHSAN N A,NURSA’ADAH R S,et al.Evaluation of glutathione production method using Saccharomyces cerevisiae[J].IOP Conference Series:Materials Science and Engineering,2019,543(1):012004.
[35] KOBAYASHI J,SASAKI D,BAMBA T,et al.Sustainable production of glutathione from lignocellulose-derived sugars using engineered Saccharomyces cerevisiae[J].Applied Microbiology and Biotechnology,2019,103(3):1 243-1 254.
[36] PATZSCHKE A,STEIGER M G,HOLZ C,et al.Enhanced glutathione production by evolutionary engineering of Saccharomyces cerevisiae strains[J].Biotechnology Journal,2015,10(11):1 719-1 726.
[37] CMARA JNIOR A A C,NGUYEN T D,JOSSIER A,et al.Improving total glutathione and trehalose contents in Saccharomyces cerevisiae cells to enhance their resistance to fluidized bed drying[J].Process Biochemistry,2018,69:45-51.
[38] LORENZ E,SCHMACHT M,STAHL U,et al.Enhanced incorporation yield of cysteine for glutathione overproduction by fed-batch fermentation of Saccharomyces cerevisiae[J].Journal of Biotechnology,2015,216:131-139.
[39] XIONG Z Q,GUO M J,CHU J,et al.On-line specific growth rate control for improving reduced glutathione production in Saccharomyces cerevisiae[J].Biotechnology and Bioprocess Engineering,2015,20(5):887-893.
[40] SCHMACHT M,LORENZ E,STAHL U,et al.Medium optimization based on yeast's elemental composition for glutathione production in Saccharomyces cerevisiae[J].Journal of Bioscience and Bioengineering,2017,123(5):555-561.
[41] CHEN H L,CAO X T,ZHU N Q,et al.A stepwise control strategy for glutathione synthesis in Saccharomyces cerevisiae based on oxidative stress and energy metabolism[J].World Journal of Microbiology and Biotechnology,2020,36(8):1-10.
[42] XU R Y,WANG D H,WANG C L,et al.Improved S-adenosylmethionine and glutathione biosynthesis by heterologous expression of an ATP6 gene in Candida utilis[J].Journal of Basic Microbiology,2018,58(10):875-882.
[43] WANG D H,LI D C,ZHANG G C,et al.Disruption of por1 gene in Candida utilis improves co-production of S-adenosylmethionine and glutathione[J].Journal of Biotechnology,2019,290:16-23.
[44] ZHANG G C,YAO X Y,WANG C L,et al.Transcriptome analysis reveals the mechanism underlying improved glutathione biosynthesis and secretion in Candida utilis during selenium enrichment[J].Journal of Biotechnology,2019,304:89-96.
[45] LI D C,WANG D H,WEI G Y.Efficient co-production of S-adenosylmethionine and glutathione by Candida utilis:effect of dissolved oxygen on enzyme activity and energy supply[J].Journal of Chemical Technology & Biotechnology,2017,92(8):2 150-2 158.
[46] GAO Y H,LIU N,ZHU Y X,et al.Improving glutathione production by engineered Pichia pastoris:Strain construction and optimal precursor feeding[J].Applied Microbiology and Biotechnology,2022,106(5):1 905-1 917.
[47] 高宇豪,吴勇杰,朱亚鑫,等.产谷胱甘肽毕赤酵母工程菌的构建及能量调控[J].食品与发酵工业,2021,47(7):21-26.GAOY H,WU Y J,ZHU Y X,et al.Construction and energy regulation of engineered glutathione-producing Pichia pastoris[J].Food and Fermentation Industries,2021,47(7):21-26.
[48] 蒋秋琪,吕雪芹,崔世修,等.代谢工程改造毕赤酵母发酵生产谷胱甘肽[J].食品与发酵工业,2020,46(17):9-14.JIANG Q Q,LU X Q,CUI S X,et al.Metabolic engineered Pichia pastoris for synthesis of glutathione[J].Food and Fermentation Industries,2020,46(17):9-14.
[49] LORENZ E,SCHMACHT M,SENZ M.Evaluation of cysteine ethyl ester as efficient inducer for glutathione overproduction in Saccharomyces spp.[J].Enzyme and Microbial Technology,2016,93:122-131.
[50] YURKIV M,KURYLENKO O,VASYLYSHYN R,et al.Gene of the transcriptional activator MET4 is involved in regulation of glutathione biosynthesis in the methylotrophic yeast Ogataea(Hansenula)polymorpha[J].FEMS Yeast Research,2018,18(2):foy004.
[51] DO D T H,FICKERS P,BEN TAHAR I.Improvement of glutathione production by a metabolically engineered Yarrowia lipolytica strain using a small-scale optimization approach[J].Biotechnology Letters,2021,43(2):407-414.
[52] 万俊,王常高,蔡俊,等.低温高压均质法提取谷胱甘肽的生产工艺研究[J].食品与机械,2013,29(4):137-140.WAN J,WANG C G,CAI J,et al.Study on production technology for extracting glutathione by high pressure homogenization at low temperature[J].Food & Machinery,2013,29(4):137-140.
[53] 冷非凡,王晓力,王永刚,等.高压蒸汽提取啤酒废酵母还原型谷胱甘肽的工艺优化[J].食品工业科技,2014,35(15):224-229.LENG F F,WANG X L,WANG Y G,et al.Optimization of extraction parameters for glutathione from beer waste brewing yeast treated by high-pressure steam(HPS)[J].Science and Technology of Food Industry,2014,35(15):224-229.
[54] MURYANTO M,ALVI N,NURDIN M,et al.Extraction of glutathione from EFB fermentation waste using methanol with sonication process[J].AIP Conference Proceedings,2017,1 904:020011.
[55] SUDIYANI Y,FAIZAL F A,FIRMANSYAH I,et al.Glutathione from Saccharomyces cerevisiae as by-product of second generation bioethanol from oil palm of empty fruit bunch fiber[J].IOP Conference Series:Materials Science and Engineering,2019,536(1):012142.
[56] 江燕斌,马建,杨建,等.一种有机溶剂沉淀分离初步提纯谷胱甘肽发酵液的方法:CN102286069B[P].2013-07-24.JIANG Y B,MA J,YANG J,et al.Method for primarily purifying glutathione fermentation liquid by precipitation and separation with organic solvent:CN102286069B[P].2013-07-24.
[57] 孙永龙,张会翔,张伟波.一种高纯度谷胱甘肽的生产方法:CN101429229B[P].2011-07-06.SUN Y L,ZHANG H X,ZHANG W B.Method for producing high-purity glutathione:CN101429229B[P].2011-07-06.
[58] WANG Y.Extraction and concentration of glutathione from yeast[D].Waterloo:University of Waterloo,2018.
[59] SATYANARAYANA T,KUNZE G.Yeast biotechnology:diversity and applications[M].Dordrecht:Springer Netherlands,2009:259-280.
[60] 刘秀继,邹群,覃先武,等.微生物生产谷胱甘肽的国内研究进展(1990—2020)[J].三峡大学学报(自然科学版),2022,44(4):90-106.LIU X J,ZOU Q,QIN X W,et al.A systematic review on microbial production of glutathione in China(1990—2020)[J].Journal of China Three Gorges University(Natural Science),2022,44(4):90-106.
[61] WANG X Q,YU H H,XING R E,et al.Characterization,preparation,and purification of marine bioactive peptides[J].Bio Med Research International,2017,2 017:9746720.
[62] BILAWAL A,JIANG Z M,LI Y,et al.Isolation,bioactivity,identification,and commercial application of soybean bioactive peptides[M]//Phytochemicals in Soybeans.[S.l.]:CRC Press,2022:217-234.
[63] WANG Y R,XIAO T H,ZHANG Z G,et al.Extraction and concentration of glutathione from yeast by membranes[J].The Canadian Journal of Chemical Engineering,2022,100:S195-S204.
[64] 凌思雨,王洲,张会敏,等.常压室温等离子体诱变与微生物微液滴培养选育谷胱甘肽高产菌株[J/OL].食品科学.(2022-06-13)[2022-08-26].http://kns.cnki.net/kcms/detail/11.2206.TS.20220613.1059.073.html.LING S Y,WANG Z,ZHANG H M,et al.Breeding of high yield glutathione strain by atmospheric and room temperature plasma mutagenesis and microbial microdroplet culture system[J/OL].Food Science.(2022-06-13)[2022-08-26].http://kns.cnki.net/kcms/detail/11.2206.TS.20220613.1059.073.html.
[65] WESCHAWALIT S,THONGTHIP S,PHUTRAKOOL P,et al.Glutathione and its antiaging and antimelanogenic effects[J].Clinical,Cosmetic and Investigational Dermatology,2017,10:147-153.
[66] ISKUSNYKH I Y,ZAKHAROVA A A,PATHAK D.Glutathione in brain disorders and aging[J].Molecules,2022,27(1):324-336.
[67] KENNEDY L,SANDHU J K,HARPER M E,et al.Role of glutathione in cancer:From mechanisms to therapies[J].Biomolecules,2020,10(10):1 429-1 456.
[68] IGHODARO O M.Molecular pathways associated with oxidative stress in diabetes mellitus[J].Biomedicine & Pharmacotherapy,2018,108:656-662.
[69] TESKEY G,ABRAHEM R,CAO R Q,et al.Glutathione as a marker for human disease[J].Advances in Clinical Chemistry,2018,87:141-159.
[70] LIM J C,GREY A C,ZAHRAEI A,et al.Age-dependent changes in glutathione metabolism pathways in the lens:New insights into therapeutic strategies to prevent cataract formation:A review[J].Clinical & Experimental Ophthalmology,2020,48(8):1 031-1 042.
[71] 郑丽雪,齐斌,孙姜,等.外源性氧化胁迫对酿酒酵母突变株Y518合成谷胱甘肽的影响[J].食品与机械,2017,33(9):15-19.ZHENG L X,QI B,SUN J,et al.Effects of exogenous oxidation stress on glutathione synthesis inSaccharomyces cerevisiae mutant Y518[J].Food & Machinery,2017,33(9):15-19.
[72] ZHANG Y,SIMPSON B K,ADI D D,et al.Indigenous milk enzymes[M]//Handbook of Dairy Foods Analysis.[S.l.]:CRC Press,2021:123-141.
[73] WU S J.Preparation of canned apple juice using glutathione as an enzymatic and non-enzymatic browning inhibitor[J].Journal of Food Processing and Preservation,2017,41(1):e12750.
[74] ZHANG X,CHU J P,DONG S X,et al.Chain terminators and glutathione weaken wheat dough under excess nitrogen input[J].Journal of Agricultural and Food Chemistry,2022,70(17):5 357-5 368.
[75] 戴庞聪,吴慧.探讨谷胱甘肽的应用研究进展[J].现代食品,2020(21):40-43.DAI P C,WU H.Research progress on the application of glutathione[J].Modern Food,2020(21):40-43.
[76] LU X,DEL PRADO D R,ARAUJO L D,et al.Effect of glutathione addition at harvest on sauvignon blanc wines[J].Australian Journal of Grape and Wine Research,2021,27(4):431-441.
[77] MEZZETTI F,DE VERO L,GIUDICI P.Evolved Saccharomyces cerevisiae wine strains with enhanced glutathione production obtained by an evolution-based strategy[J].FEMS Yeast Research,2014,14(6):977-987.
[78] 肖建才,刘建功,赵子鹤,等.传统健康食疗产业发展现状与展望[J].食品与机械,2022,38(6):8-15,236.XIAO J C,LIU J G,ZHAO Z H,et al.Status and prospects of traditional health food therapy industry development[J].Food & Machinery,2022,38(6):8-15,236.
[79] 周丹,郑建仙,黄寿恩.功能性食品研发新模式:柔性精准营养干预系统[J].食品与机械,2022,38(6):4-7,87.ZHOU D,ZHENG J X,HUANG S E.New model of functional food research and development:Flexible and precise nutrition intervention system[J].Food & Machinery,2022,38(6):4-7,87.
[80] Global Market Insight.Glutathione Market[R/OL].Selbyvile:GMI,2021.[2022-08-23].https://www.gminsights.com/industry-analysis/glutathione-market.
[81] 上海腾道信息技术有限公司.外贸通平台数据库[DB/OL].[2022-09-12].https://www.tendata.cn/t-info/.Shanghai Tendata Tech Co.,Ltd.Trader database platform[DB/OL].[2022-09-12].https://www.tendata.cn/t-info/.
[82] 杨世慧,王军,秦永发,等.谷胱甘肽生理功能、发酵生产及其在动物生产中应用的研究进展[J/OL].动物营养学报.(2022-06-17)[2022-09-06].http://kns.cnki.net/kcms/detail/11.5461.S.20220615.1443.028.html.YANG S H,WANG J,QIN Y F,et al.Research progress of physiological function and fermentation production of glutathione and its application in animal production[J/OL].Chinese Journal of Animal Nutrition.(2022-06-17)[2022-09-06].http://kns.cnki.net/kcms/detail/11.5461.S.20220615.1443.028.html.
[83] ARYADEEP R,DURGESH K T.Protective chemical agents in the amelioration of plant abiotic stress:Biochemical and molecular perspectives[M].[S.l.]:John Wiley & Sons,2020:122-146.

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