Objective: To establish an analytical method for the determination of four main flavor substances in liquor. Methods: A gas chromatography mass spectrometry (GC-MS) method was established for the simultaneous determination of esters, alcohols, acids, and aldehydes in liquor. Single ion monitoring (SIM) mode was chosen. Then the method was inspected through comparison with the standard mass spectrum database, quantitative analysis of the external standard method and relative standard deviation of day-in and day-out were investigated. Results: The limits of detection of the four types of flavor compounds in liquor were 0.007~0.070 mg/L, and the limits of quantitation were 0.020~0.200 mg/L, and the linearities were satisfactory in the range of 0.000 5~5.0 g/L, with the correlation coefficients of R2≥0.998 5. The accuracy was tested by adding three levels of low, medium and high concentrations of standard substances to ethanol in water (60%), and the average recoveries of the four categories of flavor substances were 87.8%~107.4%, with the relative standard deviations (RSDs) of 2.0%~7.4% (n=6). Conclusion: The method is accurate and reliable, with good repeatability and high sensitivity. The method is stable and can be used for the determination of various trace substances in wine. It was found that the volatile flavor compounds of different flavor liquors were different to some extent, which could provide a reference for further research on the flavor compounds of liquor.

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[1] 孙宝国,吴继红,黄明泉,等.白酒风味化学研究进展[J].中国食品学报,2015,15(9):1-8.SUN B G,WU J H,HUANG M Q,et al.Research progress on chemistry of liquor flavor[J].Journal of Chinese Institute of Food Science and Technology,2015,15(9):1-8.
[2] 孙宝国,李贺贺,胡萧梅,等.健康白酒的发展趋势[J].中国食品学报,2016,16(8):1-6.SUN B G,LI H H,HU X M,et al.The development trend of healthy liquor[J].Journal of Chinese Institute of Food Science and Technology,2016,16(8):1-6.
[3] 郭学武,范恩帝,马冰涛,等.中国白酒中微量成分研究进展[J].食品科学,2020,41(11):267-276.GUO X W,FAN E D,MA B T,et al.Recent progress in micro components of Chinese Baijiu[J].Food Science,2020,41(11):267-276.
[4] ZHU S K,LU X,LI K L,et al.Characterzation of flavor compounds in Chinese liquor moutai by comprehensive two-dimensional gas chromatographt/time-of-flightmass spectrometry[J].Analytica Chimica Acta,2007,597(2):340-348.
[5] 季克良,郭坤亮,朱书奎,等.全二维气相色谱/飞行时间质谱用于白酒微量成分的分析[J].酿酒科技,2007(3):100-102.JI K L,GUO K L,ZHU S K,et al.Analysis of microconstituents in liquor by full two-dim ensional gas chromatography/time of flight mass spectrum[J].Liquor-making Science & Technology,2007(3):100-102.
[6] 纪南,廖永红,丁芳,等.市售5种酱香型白酒挥发性风味物质的主成分分析[J].酿酒科技,2016(9):17-22,30.JI N,LIAO Y H,DING F,et al.Principal component analysis of volatile flavoring compounds in five kinds of Jiangxiang Baijiu[J]Liquor-making Science & Technology,2016(9):17-22,30.
[7] 徐军.浓香型枝江白酒香味成分的分析研究[D].武汉:华中农业大学,2019:1-20.XU J.Study on aroma components of strong-aroma Zhijiang Baijiu[D].Wuhan:Huazhong Agricultural University,2019:1-20.
[8] 邢爽.白酒发酵过程中酯类物质形成机理的研究[D].天津:天津科技大学,2018:1-10.XING S.Research of the formation mechanism of esters in process of liquor fermentation[D].Tianjin:Tianjin University of Science and Technology,2018:1-10.
[9] 廖永红,杨春霞,胡佳音,等.气相色谱—质谱法分析比较牛栏山牌清香型二锅头酒和浓香型白酒中的香味成分[J].食品科学,2012,33(6):181-185.LIAO Y H,YANG C X,HU J Y,et al.Comparison of aroma compounds in mild aromatic niulanshan erguotou liquor and strong aromatic niulanshan liquor by GC-MS[J].Food Science,2012,33(6):181-185.
[10] 邵燕,张宿义,祝成,等.浓香型白酒风味物质与感官评定相关性研究[J].中国酿造,2012,31(8):92-95.SHAO Y,ZHANG S Y,ZHU C,et al.Study on the correlation of flavor of Luzhou-flavor liquor with sensory evaluation[J].China Brewing,2012,31(8):92-95.
[11] 周玮婧,江小明.气相色谱法测定不同香型白酒中醇类与醛类物质含量[J].中国酿造,2017,36(4):180-183.ZHOU W J,JIANG X M.Determination of the alcohols and the aldehydes contents in different flavor types of Baijiu(Chinese liquor)by gas chromatography[J].China Brewing,2017,36(4):180-183.
[12] 刘莎,周玉,余义,等.气相色谱同时测定白酒中22种风味物质的方法研究[J].酿酒科技,2019(2):113-116.LIU S,ZHOU Y,YU Y,et al.Simultaneous determination of 22 flavoring substances in Baijiu by GC[J].Liquor-making Science & Technology,2019(2):113-116.
[13] 张晓婕,邱树毅,王晓丹,等.白酒挥发性风味物质气相分析方法及研究进展[J].食品与发酵工业,2021,47(14):313-320.ZHANG X J,QIU S Y,WANG X D,et al.Gas chromatography methods and research progress of volatile flavor substances in Chinese liquor(Baijiu)[J].Food and Fermentation Industries,2021,47(14):313-320.
[14] 董文静,钱承敬,穆蕾,等.顶空固相微萃取—气相色谱—质谱法分析馥郁香型白酒风味物质[J].食品安全质量检测学报,2018,9(10):2 362-2 368.DONG W J,QIAN C J,MU L,et al.Analysis of flavor compounds of Fuyu flavor liquor by head space-solid phase microextraction with gas chromatography-mass spectrometry[J].Journal of Food Safety and Quality,2018,9(10):2 362-2 368.
[15] 李俊刚,郭文宇,罗英,等.利用GC-MS法对不同窖龄下浓香型白酒风味物质的研究[J].中国酿造,2015,34(9):141-144.LI J G,GUO W Y,LUO Y,et al.Analysis of volatile compounds of Luzhou-flavor liquor from different pit ages by GC-MS[J].China Brewing,2015,34(9):141-144.
[16] FAN H Y,FAN W L,XU Y.Characterization of key odorants in Chinese chixiang aroma-type liquor by gas chromatography-olfactometry,quantitative measurements,aroma recombination,and omission studies[J].Journal of Agricultural and Food Chemistry,2015,63(14):3 660-3 668.
[17] 罗琪,张贵宇,庹先国,等.电子鼻在成品白酒检测中的应用研究进展[J].粮食与油脂,2022,35(4):4-7.LUO Q,ZHANG G Y,TUO X G,et al.Analysis of volatile components in sauce-flavor Baijiu with different ages by electronic nose and GC-MS[J].Analysis and Examination,2022,35(4):4-7.
[18] 李俊,王震,郭晓关,等.基于全二维气相飞行时间质谱联用法分析贵州酱香型白酒挥发性风味成分[J].酿酒科技,2016(12):102-106.LI J,WANG Z,GUO X G,et al.Analysis of volatile flavoring components in Guizhou Jiangxiang Baijiu by two comprehensive dimensional gas chromatography-time of flight mass spectrometry[J].Liquor-making Science & Technology,2016(12):102-106.
[19] 廖永红,赵爽,张毅斌,等.LLE、SDE、SPME和GC-MS结合保留指数法分析二锅头酒中的风味物质[J].中国食品学报,2014,14(6):220-228.LIAO Y H,ZHAO S,ZHANG Y B,et al.Analysis of flavor substances in erguotou wine by LLE,SDE,SPME and GC-MS combined with kovats retention indices[J].Journal of Chinese Institute of Food Science and Technology,2014,14(6):220-228.
[20] LIU H L,SUN B G.Effect of fermentation processing on the flavor of Baijiu[J].Journal of Agricultural and Food Chemistry,2018,66(22):5 425-5 432.
[21] 唐平,山其木格,王丽,等.白酒风味化学研究方法及酱香型白酒风味化学研究进展[J].食品科学,2020,41(17):315-324.TANG P,SHAN Q M G,WANG L,et al.A review of research methods in baijiu flavor chemistry and recent progress in the flavor chemistry of maotai-flavored Baijiu[J].Food Science,2020,41(17):315-324.
[22] 胡瑾,马一飞,罗佳雪,等.气相色谱法同时测定白酒中57种风味物质[J].中国酿造,2022,41(5):206-211.HU J,MA Y F,LUO J X,et al.Simultaneous determination of 57 flavor substances in Baijiu by GC[J].China Brewing,2022,41(5):206-211.

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