Isolation, purification and identification of ACE inhibitory peptide from Paramisgurnus dabryanus protein
Objective: This study aimed to develop ACE inhibitory peptide of Paramisgurnus dabryanus.Methods: Ultrafiltration, Sephadex G-15 and RP-HPLC were used to isolate and purify ACE inhibitory peptides from the proteolysis products of P.dabryanus,and their structures were analyzed by HPLC-MS/MS. The inhibitory mode of purified ACE inhibitory peptide was analyzed by inhibition kinetics. Results: The amino acid sequence of ACE inhibitory peptide was Glu-Gly-HIS, the relative molecular weight was 341.2 Da, the IC 50 value was (17.89±3.28)μg/mL, and the purification ratio was 27.47. Conclusion: The inhibitory peptide of P. dabryanus is a competitive ACE inhibitory peptide.
Jing-qi, DONG; Ze-he, HE; Gui-fang, TIAN; Juan-juan, SHAO; and Xiao-he, WU
"Isolation, purification and identification of ACE inhibitory peptide from Paramisgurnus dabryanus protein,"
Food and Machinery: Vol. 38:
12, Article 24.
Available at: https://www.ifoodmm.cn/journal/vol38/iss12/24
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