Abstract
Objective: This study aimed to develop ACE inhibitory peptide of Paramisgurnus dabryanus.Methods: Ultrafiltration, Sephadex G-15 and RP-HPLC were used to isolate and purify ACE inhibitory peptides from the proteolysis products of P.dabryanus,and their structures were analyzed by HPLC-MS/MS. The inhibitory mode of purified ACE inhibitory peptide was analyzed by inhibition kinetics. Results: The amino acid sequence of ACE inhibitory peptide was Glu-Gly-HIS, the relative molecular weight was 341.2 Da, the IC 50 value was (17.89±3.28)μg/mL, and the purification ratio was 27.47. Conclusion: The inhibitory peptide of P. dabryanus is a competitive ACE inhibitory peptide.
Publication Date
12-28-2022
First Page
145
Last Page
150
DOI
10.13652/j.spjx.1003.5788.2022.90087
Recommended Citation
Jing-qi, DONG; Ze-he, HE; Gui-fang, TIAN; Juan-juan, SHAO; and Xiao-he, WU
(2022)
"Isolation, purification and identification of ACE inhibitory peptide from Paramisgurnus dabryanus protein,"
Food and Machinery: Vol. 38:
Iss.
12, Article 24.
DOI: 10.13652/j.spjx.1003.5788.2022.90087
Available at:
https://www.ifoodmm.cn/journal/vol38/iss12/24
References
[1] CHRISTINA G K,DIMITRIOS P B,LAZAROS I S.Inflammation and cardiovascular disease[J].World Journal of Translational Medicine,2019,8(1):1-8.
[2] KAHSU G A,MASRESHA A B,MEHARI A T,et al.Targeting renin-angiotensin system against alzheimer s disease[J].Frontiers in Pharmacology,2018,9:440-456.
[3] CLAYTON D,HANCHAPOLA I,THOMAS W G,et al.Structural determinants for binding to angiotensin converting enzyme 2(ACE2)and angiotensin receptors 1 and 2[J].Frontiers in Pharmacology,2015,6:5-13.
[4] XUE L,WANG X D,HU Z H,et al.Identification and characterization of an angiotensin-converting enzyme inhibitory peptide derived from bovine casein[J].Peptides,2018,99:161-168.
[5] ILA J,JANAGARAJ K,PEER M.Isolation and characterization of angiotensin I-converting enzyme(ACE-I)inhibition and antioxidant peptide from by-catch shrimp(Oratosquilla woodmasoni)waste[J].Biocatalysis and Agricultural Biotechnology,2020,29:101770.
[6] 姚金明,陈秀梅,刘明哲,等.饲料中添加虾青素对大鳞副泥鳅生长和体色的影响[J].饲料工业,2019,40(8):46-51.YAO J M,CHEN X M,LIU M Z,et al.Effects of Astaxanthin on the growth and body color of juvenile Paramisgurnus dabryanus [J].Feed Industry,2019,40(8):46-51.
[7] 何泽贺,肖宇,刘建朝,等.大鳞副泥鳅蛋白制备ACE抑制肽的酶解方式研究[J].食品研究与开发,2021,42(7):134-140.HE Z H,XIAO Y,LIU J C,et al.Study on enzymatic hydrolysis of ACE inhibitory peptide prepared from Paragurnus dhamgurnus protein[J].Food Research and Development,2021,42(7):134-140.
[8] HUANG Y O,RUAN G H,QIN Z J,et al.Antioxidant activity measurement and potential antioxidant peptides exploration from hydrolysates of novel continuous microwave-assisted enzymolysis of the Scomberomorus niphonius protein[J].Food Chemistry,2017,223:89-95.
[9] THEODRI P J,TICKEL S,IDIENS R.Proline specific protease from Penicillium Chrysogenum:200980120102.4[P].2011-05-04.
[10] JANG A,LEE M.Purification and identification of angiotensin converting enzyme inhibitory peptides from beef hydrolysates[J].Meat Science,2005,69(4):653-661.
[11] LI M Y,FAN W L,XU Y.Identification of angiotensin converting enzyme(ACE)inhibitory and antioxidant peptides derived from Pixian broad bean paste[J].LWT-Food Science and Technology,2021,151:112221.
[12] 石杰,宿瑞奇,张文婷,等.三斑海马蛋白ACE抑制肽的制备及其二级结构的研究[J].食品研究与开发,2020,41(9):38-44.SHI J,SU Q Q,ZHANG W T,et al.Preparation and secondary structure of angiotensin i-converting enzyme(ACE)inhibitory peptide of Seahorse protein[J].Food Research and Development,2020,41(9):38-44.
[13] 李锐,邹茜,孙玉林,等.克氏原螯虾虾头模拟胃肠道消化产物中ACE抑制肽的分离纯化与鉴定[J].食品与发酵工业,2019,45(6):139-146.LI R,ZOU Q,SUN Y L,et al.Isolation and identification of ACE inhibitory peptide from simulated gastrointestinal digestive products of Procambarus clarkii head[J].Food and Fermentation Industries,2019,45(6):139-146.
[14] WU J,ALUKO R E,NAKAI S.Structural requirements of angiotensin I-converting enzyme inhibitory peptides:Quantitative structure-activity relationship study of di-and tripeptides[J].Journal of Agricultural and Food Chemistry,2006,54(3):732-738.
[15] LI Y,ZHOU J Z,HUANG K H,et al.Purification of a novel angiotensin-Ⅰ-converting enzyme(ACE)inhibitory peptide with an antihypertensive effect from loach(Misgurnus anguillicaudatus)[J].Journal of Agricultural and Food Chemistry,2012,60(5):1 320-1 325.
[16] LEE S H,QIAN Z J,KIM S K,et al.A novel angiotensin-I-converting enzyme inhibitory peptide from tuna frame protein hydrolysate and its antihypertensive effect in spontaneously hypertensive rats[J].Food Chemistry,2010,118(1):96-102.
[17] FORGHANI B,ZAREI M,EBRAHIMPOUR A,et al.Purification and characterization of angiotensin converting enzyme-inhibitory peptides derived from Stichopus horrens:Stability study against the ACE and inhibition kinetics[J].Journal of Functional Foods,2016,20:276-290.
[18] HENDAY B,LABIDI A,ARNAUDIN I,et al.Measuring angiotensin-I-converting enzyme inhibitory activity by micro plate assays:Comparison using marine cryptides and tentative threshold determinations with captopril and losartan[J].Food Chemistry,2013,61:10 685-10 690.
[19] RUIZ R J,DAVILA O G,CHEL G L,et al.Angiotensin-I-converting enzyme inhibitory and antioxidant peptide fractions from hard-to-cook bean enzymatic hydrolysates[J].Journal of Food Biochemistry,2013,37(1):26-35.
[20] 卢洋.核桃蛋白源ACE抑制肽的纯化、结构鉴定及性质研究[D].西安:陕西科技大学,2015:80.LU Y.Purification,identification and characterization of angiotensin i-converting enzyme inhibitory peptide derived from Walnut protein[D].Xi an:Shaanxi University of Science and Technology,2015:80.