Objective:To study the protective effects of exo-polysaccharide of Rhodotorula mucilaginosa on drug-induced liver injury in mice and analyze its potential molecular mechanism, so as to provide scientific basis for the application of exo-polysaccharide of Rhodotorula mucilaginosa in functional food and drugs.Methods:Based on the antioxidant activity of polysaccharides, the effects of exo-polysaccharide component RmEPS-11 from Rhodotorula mucilaginosa on liver toxicity induced by INH and RIF in mice were evaluated.Results:Compared with the mice treated with INH and RIF, the levels of ALT and AST in serum and the content of MDA in liver were significantly decreased, while the activity of SOD and the content of GSH in liver were significantly restored. In addition, the histopathological damage of liver and the number of apoptotic hepatocytes were also significantly improved. The mechanism study showed that the protective effect was mainly due to the regulation of CYP2E1 mRNA expression level, resulting in the reduction of harmful free radicals and ROS formation, accompanied by the significant induction of antioxidant capacity.Conclusion:The polysaccharide RmEPS-11 extracted from R. mucilaginosa CM-1 can protect mice from acute liver injury induced by INH and RIF.

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[1] 杨博,李婧,杨长青.药物性肝损伤的易感因素研究进展[J].肝脏,2018,23(9):78-80.YANG Bo,LI Jing,YANG Chang-qing.Researchprogress on susceptibility factors of drug-induced liver injury[J].Liver,2018,23(9):78-80.
[2] 王菲,王允野.引起肝损害的药物及临床治疗[J].中国处方药,2015(9):9-10.WANG Fei,WANG Yun-ye.Drugs causing liver damage and clinical treatment[J].Chinese Prescription Drugs,2015(9):9-10.
[3] GILDOMAR L,VALASQUES Junior.Extraction optimization and antinociceptive activity of(1→3)-β-d-glucan from Rhodotorulamucilaginosa[J].Carbohydrate Polymers,2014,105(1):293-299.
[4] MA Wen-jin,CHEN Xue-feng,WANG Bo,et al.Characterization,antioxidativity,and anti-carcinoma activity of exopolysaccharide extract from Rhodotorula mucilaginosa CICC 33013[J].Carbohydrate Polymers,2018,181:768-777.
[5] 马文锦,李梅林,王博,等.胶红酵母Rhodotorula mucilaginosa CICC 33013胞外多糖的分离纯化及抗氧化活性研究[J].食品与发酵工业,2019,45(11):65-70.MA Wen-jin,LI Mei-lin,WANG Bo,et al.Purification and antioxidant activity of exopolysaccharide from Rhodotorula mucilaginosa CICC 33013[J].Food and Fermentation Industry,2019,45(11):65-70.
[6] 朱亚男,徐菘阳,丁慧敏,等.白玉菇多糖对免疫抑制型小鼠的免疫调节作用[J].食品工业科技,2020(7):295-300.ZHU Ya-nan,XU Song-yang,DING Hui-min,et al.Immunomodulatoryeffects of white hypsizygus marmoreus polysaccharides on immunosuppressive mice[J].Science and Technology of Food Industry,2020(7):295-300.
[7] WANG Wen-cheng,LI Jin-lian,WANG Zhi,et al.Oral hepatoprotective ability evaluation of purple sweet potato anthocyanins on acute and chronic chemical liver injuries[J].Cell Biochemistry & Biophysics,2014,69(3):539-548.
[8] 韦秀桂,王红园,黄秀昆,等.老鼠簕生物碱A对小鼠药物性肝损伤的保护作用[J].中国实验方剂学杂志,2019(15):103-107.WEI Xiu-gui,WANG Hong-yuan,HUANG Xiu-kun,et al.Protective effect of Acanthus ilicifolius alkaloid a on drug-induced liver injury in mice[J].Chinese Journal of Experimental Prescriptions,2019(15):103-107.
[9] 郑俏然,张恒,李文峰,等.美味牛肝菌多糖对急性肝损伤小鼠的保肝作用[J].食品与机械,2019,35(12):141-145.ZHENG Qiao-ran,ZHANG Heng,LI Wen-feng,et al.Hepatoprotective effect of Boletus edulis polysaccharideon mice with acute liver injury[J].Food & Machinery,2019,35(12):141-145.
[10] 张扬,朱彩平,林杨楠,等.平菇多糖对四氯化碳诱导雄性昆明种小鼠肝损伤的保护作用[J].食品科学,2019,40(23):157-162.ZHANG Yang,ZHU Cai-ping,LIN Yang-nan,et al.Hepatoprotective effect of pleurotus ostreatus polysaccharides on carbon tetrachloride-induced liver injury in male Kunming mice[J].Food Science,2019,40(23):157-162.
[11] 林素珍,胡臻,金斌斌,等.升清降糖合剂对1型糖尿病小鼠胰岛细胞形态功能及凋亡相关基因的影响[J].中华中医药学刊,2016(9):2 253-2 257.LIN Su-zhen,HU Zhen,JIN Bin-bin,et al.Effect of Shengqing Jiangtang mixture on morphology and function of pancreatic islet cells and apoptosis related genes in type 1 diabetic mice[J].Chinese Journal of traditional Chinese medicine,2016(9):2 253-2 257.
[12] 陈小凤,曾奇峰,邓旭,等.一种大量提取红豆杉中总RNA的方法研究[J].生物技术通报,2017,33(11):60-66.CHEN Xiao-feng,ZENG Qi-feng,DENG Xu,et al.Study on a method of extracting total RNA from Taxus chinensis[J].Biotechnology Bulletin,2017,33(11):60-66.
[13] GREEN M R,SAMBROOK J.Purification of total RNA from mammalian cells and tissues[J].Cold Spring Harbor Protocols,2020(1):101659.
[14] 王颖芳,陈艳琳,王文娟.适用于转录组测序的人参根总RNA提取方法的筛选[J].广东药科大学学报,2017,33(1):18-22.WANG Yin-fang,CHEN Yan-lin,WANG Wen-juan.Screening of total RNA extraction methods from Panax ginseng root for transcriptome sequencing[J].Journal of Guangdong Pharmaceutical University,2017,33(1):18-22.
[15] 张树伟,丁峰,黄羽,等.适用于转录组测序的毛葡萄叶片总RNA提取方法筛选[J].分子植物育种,2020,18(11):147-151.ZHANG Shu-wei,DING Feng,HUANG Yu,et al.Screening of total RNA extraction methods for transcriptome sequencing of Vitis tomentosa[J].Molecular Plant Breeding,2020,18(11):147-151.
[16] 刘遥,王宪波.药物性肝损伤常见药物发病机制的研究进展[J].中西医结合肝病杂志,2020,30(1):20-23.WANG Yao,WANG Xian-bo.Research progress on the pathogenesis of common drugs in drug-induced liver injury[J].Journal of Integrated Traditional Chinese and Western Medicine Liver Disease,2020,30(1):20-23.
[17] UENG Y F,SHYU C C,LIN Y L,et al.Effects of baicalein and wogonin on drug-metabolizing enzymes in C57BL/6J mice[J].Life Sciences,2000,67(18):2 189-2 200.
[18] LEE S S T,BUTERS J T M,PINEAU T,et al.Role of CYP2E1 in the hepatotoxicity of acetaminophen[J].The Journal of Biological Chemistry,1996,271(20):12 063-12 067.
[19] 郭丁妮,吴红海,薛改,等.异烟肼和利福平致小鼠原代肝细胞损伤时CYP2E1和CYP3A酶活性的变化[J].解放军药学学报,2011(3):221-223.GUO Ding-ni,WU Hong-hai,XUE Gai,et al.Thehepatocyte toxicity and the effect of isoniazid and rifampicin on the activities of CYP2E1 and CYP3A in mouse prim ary hepatocytes[J].Journal of PLA Pharmacy,2011(3):221-223.
[20] LIAN Yong,ZHAN Jing,XU Pei-yu,et al.Protective effects of metallothionein on isoniazid and rifampicin-induced hepatotoxicity in mice[J].PLoS One,2013,8(8):e72058.



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