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Abstract

Objective: This study aimed to verify the antialcoholic activity of ovalbumin peptide and improve the added value of egg products. Methods: Ovalbumin was hydrolyzed by alkaline protease and trypsin. The activity rate of alcohol dehydrogenase (ADH) was taken as index, and the enzymolysis process was optimized by response surface methodology. The total antioxidant activity, hydrolysis degree, amino acid composition and relative molecular weight of ovalbumin peptide were determined to evaluate the antialcoholic effect of ovalbumin peptide. Results: The optimum enzymatic hydrolysis conditions were as follows. Under the conditions of enzymatic hydrolysis time 5 h, dosage of complex enzyme 3 100 U/g, enzymatic hydrolysis temperature 50 ℃, ratio of solid to liquid movalbuminVdistilled water=3.5∶25 (g/mL), the ADH activation rate of ovalbumin peptide was (22.71±0.15)%, and the total antioxidant capacity was (9.51±0.03) U/mL. The degree of hydrolysis was (29.28±0.16)%. Ovalbumin peptide was rich in amino acids such as glutamic acid (9.553 4%), alanine (3.863 3%), leucine (5.764 7%), which was beneficial to improve the activity of antialcoholic, and the content of small molecule peptide (<1 000 Da) was more than 88.77%. Conclusion: The ovalbumin peptide prepared by complex enzymatic hydrolysis method has good antioxidant activity and antialcoholic effect.

Publication Date

6-30-2022

First Page

166

Last Page

172

DOI

10.13652/j.spjx.1003.5788.2022.60004

References

[1] ZHAO Rui-Jie,HUO Chun-Yan,QIAN Yang,et al.Ultra-high-pressure processing improves proteolysis and release of bioactive peptides with activation activities on alcohol metabolic enzymes in vitro from mushroom foot protein[J].Food Chemistry,2017,231:25-32.
[2] 骆静.牡蛎蛋白小分子肽制备关键技术研究[D].舟山:浙江海洋大学,2019:1-2.LUO Jing.Study on key technology of preparation of oyster protein small molecular peptide[D].Zhoushan:Zhejiang Ocean University,2019:1-2.
[3] MA Zhi-li,ZHANG Wen-jun,YU Guo-cai,et al.The primary structure identification of a corn peptide facilitating alcohol metabolism by HPLC-MS/MS[J].Peptides,2012,37(1):138-143.
[4] 赵谋明,马梅,苏国万,等.具有醒酒活性的玉米肽的制备、富集和鉴定[J].中国食品学报,2020,20(9):86-94.ZHAO Mou-ming,MA Mei,SU Guo-wan,et al.Preparation enrichment and identification of corn peptides with sobering activity[J].Chinese Journal of Food,2020,20(9):86-94.
[5] SHI Jie,ZHOU Xin,ZHAO Ying,et al.The three-spot seahorse-derived peptide PAGPRGPA attenuates ethanol-induced oxidative stress in LO2 cells through MAPKs,the Keap1/Nrf2 signalling pathway and amino acid metabolism[J].Food & Function,2021,12(4):1 672-1 687.
[6] MA Zhi-li,HOU Tao,SHI Wen,et al.Purification and identification of corn peptides that facilitate alcohol metabolism by semi-preparative high-performance liquid chromatography and nano liquid chromatography with electrospray ionization tandem mass spectrometry[J].Journal of Separation Science,2016,39(21):4 234-4 242.
[7] 赵换维.杏鲍菇多肽制备工艺及体外活性研究[D].西安:西北大学,2019:7-8.ZHAO Huan-wei.Study on preparation technology and the activity of polypeptide from Pleurotus eryngii in vitro[D].Xi'an:Northwestern University,2019:7-8.
[8] JULIAN W.Egg nutrition and biotechnology[J].British Journal of Nutrition,2001,85(3):407-408.
[9] ABEYRATHNE E D N S,HUANG X,AHN D U.Antioxidant,angiotensin-converting enzyme inhibitory activity and other functional properties of egg white proteins and their derived peptides:A review[J].Poultry Science,2018,97(4):1 462-1 468.
[10] LI D,BALESANO A,JORDAN S,et al.The role of alcohol dehydrogenase in drug metabolism:Beyond ethanol oxidation[J].The AAPS Journal,2021,23(20):1-21.
[11] 刘鹏.低分子量促乙醇代谢玉米肽制备与蛋白成分对活性贡献关系研究[D].广州:华南理工大学,2015:17-18.LIU Peng.Preparation of corn originated low-molecular-weight peptides with anti-alcoholism activity and the contribution of different protein components to activity[D].Guangzhou:South China University of Technology,2015:17-18.
[12] 秦婧.卵白蛋白源抗氧化活性肽的酶法制备及分离鉴定[D].西安:陕西科技大学,2014:18-19.QIN Jing.Enzymatic preparation,isolation and identification of antioxidant peptides derived from ovalbumin[D].Xi'an:Shaanxi University of Science and Technology,2014:18-19.
[13] 徐明生.鸡蛋卵白蛋白酶解物抗氧化肽研究[D].西安:陕西师范大学,2006:36-37.XU Ming-sheng.Study on antioxidant peptides of egg white protease hydrolysate[D].Xi'an:Shaanxi Normal University,2006:36-37.
[14] 聂凌鸿,冯怡秋.鳝鱼皮胶原蛋白肽的制备及其抗氧化性能的研究[J].中国食品添加剂,2017(11):113-118.NIE Ling-hong,FENG Yi-qiu.Preparation of collagen peptide from eel skin and its antioxidant activity[J].China Food Additives,2017(11):113-118.
[15] KU S K,CHOUNG J J,KIM J K.Enhancement of alcohol metabolism by ginseng berry extract and its mixed herbal beverages:In vitro and in vivo experiments[J].Toxicology and Environmental Health Sciences,2019,11(2):144-154.
[16] 蒋婧.蛋白质水解度测定及其标准曲线绘制研究[J].广东轻工职业技术学院学报,2012,11(1):4-6.JIANG Jing.Determination of protein hydrolysis degree and drawing of standard curve[J].Journal of Guangdong Vocational and Technical College of Light Industry,2012,11(1):4-6.
[17] 蔡转章.豆粕源抗氧化肽对急性酒精性肝损伤的预防作用及机制研究[D].长春:吉林大学,2021:23-25.CAI Zhuan-zhang.Preventive effect and mechanism of soybean meal derived antioxidant peptides on acute alcoholic liver injury[D].Changchun:Jilin University,2021:23-25.
[18] CHIA C S B.A Review on the Metabolism of 25 Peptide Drugs[J].International Journal of Peptide Research and Therapeutics,2021,27(2):1 397-1 418.
[19] HABEEBULLAH S F K,ALAGARSAMY S,SATTARI Z,et al.Effect of enzymatic hydrolysis on the antioxidant activity of red and green seaweeds and characterization of the active extracts[J].Journal of the American Oil Chemists' Society,2021,98(2):185-200.
[20] GARCÍA A V.Enzymatic hydrolysis and fermentation of pea protein isolate and its effects on antigenic proteins,functional properties,and sensory profile[J].Foods,2022,11(1):118.
[21] WU Ying-mei,XU Long-quan,LIU Xiao-yi,et al.Effect of thermosonication treatment on blueberry juice quality:Total phenolics,flavonoids,anthocyanin,and antioxidant activity[J].LWT-Food Science & Technology,2021,150:112021.
[22] 李珂,杨秀华,扈麟,等.酶解骨胶原多肽的抗氧化特性研究[J].天然产物研究与开发,2010,22(2):311-314.LI Ke,YANG Xiu-hua,HU Lin,et al.Study on antioxidant properties of enzymatic hydrolyzed bone collagen peptides[J].Research and Development of Natural Products,2010,22(2):311-314.
[23] 王玉华,高晶,张静,等.鼠李糖乳杆菌B10通过抗氧化途径改善酒精性肝损伤的研究[J].食品科学,2012,33(23):270-273.WANG Yu-hua,GAO Jing,ZHANG Jing,et al.Improvement of alcoholic liver injury by Lactobacillus rhamnosus B10 through antioxidant pathway[J].Food Science,2012,33(23):270-273.
[24] 翟硕,张海悦,田田.黑豆多肽的制备及其对乙醇脱氢酶活性的影响研究[J].食品安全质量检测学报,2016,7(12):4 864-4 869.ZHAI Shuo,ZHANG Hai-yue,TIAN Tian.Preparation of black bean polypeptide and its effect on ethanol dehydrogenase activity[J].Journal of Food Safety and Quality Inspection,2016,7(12):4 864-4 869.

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