Objective:This study aimed to investigate the protective effect of dihydromyricetin (DHM) and its derivatives on oxidative damage of hepatocytes.Methods:The hydrogen peroxide damage model of L02 cells was established. DHM derivatives with different lipophilicity were obtained by enzymatic acylation of DHM. The levels of reactive oxygen species (ROS), lactate dehydrogenase (LDH), malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and mitochondrial membrane potential and Caspase 3 were measured to evaluate the protective effect of DHM and its derivatives on damaged hepatocytes.Results:Except for 3-O-lauroyl-DHM, the level of reactive oxygen species (ROS) the release of lactate dehydrogenase (LDH) and the level of malondialdehyde (MDA) and Caspase 3 in damaged hepatocytes treated with other derivatives decreased significantly, while the levels of antioxidant enzymes and the mitochondrial membrane potential increased. Of which, 3-O-octanoyl-DHM showed the best protective effect.Conclusion:Dihydromyricetin derivatives with medium chain length have better liver protection effect.

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[1] 郑洁静,续洁琨,江涛,等.藤茶总黄酮对拘束负荷引起小鼠肝损伤的保护作用[J].中国药理学通报,2006,22(10):1 249-1 253.ZHENG Jie-jing,XU Jie-kun,JIANG Tao,et al.Protective effect of total flavonoids of rattan tea on liver injury induced by restraint load in mice[J].Chinese Pharmacological Bulletin,2006,22(10):1 249-1 253.
[2] 张瑜,田静.二氢杨梅素调节糖脂代谢及其在食品中的应用[J].食品安全导刊,2020(3):147-148.ZHANG Yu,TIAN Jing.Dihydromyricetin regulates glucose and lipid metabolism and its application in food[J].China Food Safety Magazine,2020(3):147-148.
[3] HU H C,LUO F,WANG M J,et al.New method for extracting and purifying dihydromyricetin from ampelopsis grossedentata[J].ACS Omega,2020,5(23):13 955-13 962.
[4] WANG L L,QIN Y C,WANG Y B,et al.Interaction between iron and dihydromyricetin extracted from vine tea[J].Food Science & Nutrition,2020,8(11):5 926-5 933.
[5] 屠梦珏,魏进歌,陈鑫,等.二氢杨梅素对APAP诱导小鼠急性肝损伤的保护作用[J].中国药理学通报,2018,34(12):1 707-1 712.TU Meng-jue,WEI Jin-ge,CHEN Xin,et al.Protective effect of Dihydromyricetin on APAP induced acute liver injury in mice[J].Chinese Pharmacological Bulletin,2018,34(12):1 707-1 712.
[6] XIE C F,CHEN Z,ZHANG C F,et al.Dihydromyricetin amelioratrs oleic acid-induced lipid accumulation in L02 and HepG2 cells by inhibiting lipogenesis and oxidative stress[J].Life Sciences,2016,157:131-139.
[7] LI W,WU H,LIU B G,et al.Highly efficient and regioselective synthesis of dihydromyricetin esters by immobilized lipase[J].Journal of Biotechnology,2015,199:31-37.
[8] ZHU S,WANG S,CHEN S W,et al.Lipase-catalyzed highly regioselective synthesis of acylated chlorogenic acid[J].Food Bioscience,2020,37:100706.
[9] ZHAO X Y,DOU M M,ZHANG Z H,et al.Protective effect of Dendrobium officinale polysaccharides on H2O2-induced injury in H9c2 cardiomyocytes[J].Biomed Pharmacother,2017,94:72-78.
[10] CHANG X W,DONG S,BAI W L,et al.Methylated metabolites of chicoric acid ameliorate hydrogen peroxide(H2O2)-induced oxidative stress in HepG2 cells[J].Jounal of Agricultural and Food Chemistry,2021,69(7):2 179-2 189.

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