Objective: A rapid method for determination of bongkrekic acid in Auricularia auricular was developed using QuEChERS in combination with UHPLC-MS/MS. Methods: In this study, Several important experimental parameters, including type of extraction solvent, extraction time, adsorbent type and adsorbent dosage were effectively optimized using spiked samples. The sample were shaken with methanol containing 1% ammonia water, and then carried out by the C18 adsorbent purification.Results: The bongkrekic acid was determined within 4 minutes by negative ion multiple reaction monitoring mode analysis and external standard method. The linear range was 0.05~10.00 ng/mL (r>0.998 3). The limit of detection (LOD) was 2 μg/kg, respectively. The average recovery rate at different levels(low, medium and high) the intra-batch (n=6) was 74.4%~91.6%, the inter-batch (n=3) were 74.9%~89.4% with a RSD ranging from 3.3%~9.2%.Conclusion: The developed method shows simple, rapid, excellent recovery and stable repeatability, and the limit of detection lower than the existing detection standards. It is suitable for the effective detection of bongkrekic acid in Auricularia auricular samples.

Publication Date


First Page


Last Page





[1] 胡文娟,陈晓明,王玉华,等.酵米面黄杆菌毒素A的提纯及鉴定[J].卫生研究,1984(4):34-37.HU W J,CHEN X M,WANG Y H,et al.Purification and identification of Flavobacterium toxin A from fermented rice flour[J].J Hyg Res,1984(4):34-37.
[2] LI J H,ZHOU L U,LONG C Y,et al.An investigation of bongkrekic acid poisoning caused by consumption of a nonfermented rice noodle product without noticeable signs of spoilage[J].Journal of Food Protection,2019,82(10):1 650-1 654.
[3] 王海燕,宋曼丹,王建,等.广东省首起米粉米酵菌酸中毒病原菌鉴定研究[J].中国食品卫生杂志,2019,31(4):100-104.WANG H Y,SONG M D,WANG J,et al.Identification of the pathogen in rice noodles in relation to food poisoning caused by bongkrekic acid in Guangdong Province[J].Chin J Food Hyg,2019,31(4):100-104.
[4] 范璐,栾杰.云南省一例唐菖蒲伯克霍尔德氏菌(椰毒假单胞菌酵米面亚种)食物中毒事件调查分析[J].食品安全质量检测学报,2019,10(23):8 098-8 101.FAN L,LUAN J.Investigation and analysis for an event of Burkholderiagladioli(Pseudomonas cocovenenans subtype Farino fermentans)food poison in Yunnan province[J].J Food Saf Qual,2019,10(23):8 098-8 101.
[5] 申屠平平,朱珈慧,徐小民,等.一起椰毒假单胞菌酵米面亚种引起的食物中毒调查[J].上海预防医学,2019(6):466-468.SHENTU P P,ZHU J H,XU X M,et al.A food poisoning incident caused by pseudomonas cocovenenans subspfarinofermentans[J].Shanghai J Prev Med,2019(6):466-468.
[6] 中华人民共和国农业农村部.绿色食品食用菌:NY/T 749—2018[S].北京:中国农业出版社,2018.Ministry of Agriculture and Rural Development of the Peopleʼs Republic of China:Green food:Edible mushroom:NY/T 749—2018[S].Beijing:China Agricultural Press,2014.
[7] 中华人民共和国国家卫生和计划生育委员会.食品安全国家标准食用菌及其制品:GB 7096—2014[S].北京:中国标准出版社,2014.State Health and Family Planning Commission of the Peopleʼs Republic of China.National food safety standard:Edible Fungi and its products:GB 7096—2014[S].Beijing:China Standards Press,2014.
[8] 中华人民共和国国家食品药品监督管理总局,中华人民共和国国家卫生和计划生育委员会.食品安全国家标准食品中米酵菌酸的测定:GB 5009.189—2016[S].北京:中国标准出版社,2016.State Food and Drug Administration of the Peopleʼs Republic of China,State Health and Family Planning Commission of the Peopleʼs Republic of China.Food safety national standard:Determination of rice yeast acid in food safety national standard:GB 5009.189—2016[S].Beijing:China Standards Press,2016.
[9] 胡文娟,陈晓明,王玉华.酵米面、银耳、玉米中黄杆菌毒素A的薄层及高压液相色谱测定法[J].卫生研究,1986(2):33-36.HU W J,CHEN X M,WANG Y H.Determination of flavotoxin a in fermented rice flour,tremella fuciformis and corn by TLC and HPLC[J].J Hyg Res,1986(2):33-36.
[10] 王夏,孟昭赫,胡文娟,等.酵米面黄杆菌毒素A紫外分光光度测定法[J].卫生研究,1986(3):25-27.WANG X,MENG Z H,HU W J,et al.Determination of xanthobacterin a by UV Spectrophotometry[J].J Hyg Res,1986(3):25-27.
[11] 李红艳,金燕飞,黄海智,等.高效液相色谱—二极管阵列检测器结合固相萃取法快速测定食品中米酵菌酸残留[J].食品科学,2016,37(24):247-251.LI H Y,JIN Y F,HUANG H Z,et al.Fast determination of bongkrekic acid in foods using mixed-mode weak anion exchange solid phase extraction coupled with high performance liquid chromatography with diode array detection(HPLC-DAD)[J].Food Sci,2016,37(24):247-251.
[12] 侯佰立.固相萃取—高效液相色谱法快速测定食品中米酵菌酸残留[J].现代食品,2019(9):169-172.HOU B L.Fast determination of bongkrekic acid in foods using solid phase extraction-high performance liquid chromatography[J].Mod Food,2019(9):169-172.
[13] 覃冬杰,陈荣珍,卢艺,等.超高效液相色谱—串联质谱法测定柳州螺蛳粉中米酵菌酸[J].食品安全质量检测学报,2020,11(13):4 273-4 278.QIN D J,CHEN R Z,LU Y,et al.Determination of bongkrekic acid in Liuzhou river snails rice noodle by ultra performance liquid chromatography-tandem mass spectrometry[J].J Food Saf Qual,2020,11(13):4 273-4 278.
[14] 曾令浩,李思颖,李文倩,等.高效液相色谱—串联质谱法快速检测银耳中的米酵菌酸[J].食品安全质量检测学报,2019,10(19):6 538-6 541.ZENG L H,LI S Y,LI W Q,et al.Rapid determination of bongkrekic acid in Tremella by high performance liquid chromatography-tandem mass spectrometry[J].J Food Saf Qual,2019,10(19):6 538-6 541.
[15] 周鹏.超高效液相色谱串联质谱法测定银耳中米酵菌酸[J].食品研究与开发,2015,36(22):123-126.ZHOU P.Determination ofbongkrekic acid in tremella fuciformis berk by ultra high performance liquid chromatography-tandem mass spectrometry[J].Food Res Dev,2015,36(22):123-126.
[16] 李红娜,袁飞,张辰陽,等.液相色谱和飞行时间质谱联用检测米酵菌酸[J].食品工业,2018,39(7):319-323.LI H N,YUAN F,ZHANG C Y,et al.The research of detection bongkrekic acid(BA)by LC-ESI-TOF technology[J].Food Ind,2018,39(7):319-323.
[17] 黄小波,刘维平.基于QuEChERS的UHPLC-MS/MS同时检测鸡蛋中19种农药残留量[J].食品与机械,2021,37(10):56-60.HUANG X B,PAN X H.Simultaneous determination of 19 kinds of pesticide residues in eggs by UHPLC-MS/MS based on QuEChERS[J].Food & Machinery,2021,37(10):56-60.
[18] 陈思敏,林腾奕,李南,等.QuEChERS结合气相色谱三重四极杆串联质谱法测定大白菜中80种农药残留[J].食品安全质量检测学报,2020,11(16):82-93.CHEN S M,LIN T Y,LI N,et al.Detection of 80 pesticide residues in cabbage by QuEChERS combined with gas chromatography triple quadrupole mass spectrometry[J].J Food Saf Qual,2020,11(16):82-93.
[19] ORSO D,FLORIANO L,RIBEIRO L C,et al.Simultaneous determination of multiclass pesticides and antibiotics in honey samples based on ultra-high performance liquid chromatography-tandem mass spectrometry[J].Food Analytical Methods,2016,9(6):1 638-1 653.
[20] 易守福,梁锋,何青科,等.QuEChERS-气相色谱—三重四极杆质谱法同时测定黑茶中10种酰胺类除草剂残留[J].食品与机械,2021,37(8):57-62.YI S F,LIANG F,HE Q K,et al.Simultaneous determination of 10 kinds of amide herbicide residues in dark tea by QuEChERS with gas chromatography-triple quadrupole mass spectrometry[J].Food & Machinery,2021,37(8):57-62.
[21] 王立琦,贺利民,曾振灵,等.液相色谱—串联质谱检测兽药残留中的基质效应研究进展[J].质谱学报,2011,32(6):321-332.WANG L Q,HE L M,ZENG Z L,et al.Progress in matrix effect of veterinary drug residues analysis by high-performance liquid chromatography tandem mass spectrometry[J].J Chin Mass Spectr Soc,2011,32(6):321-332.

Included in

Food Science Commons



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.