This paper reviews the structure and stability of triplex nucleic acid, the progress of triplex nucleic acid-based fluorescence, electrochemical, colorimetric platform in detecting food borne hazards, and discusses the challenges and future development prospect of triplex nucleic acid-based detection technology in food safety field.

Publication Date


First Page


Last Page





[1] CHAZELAS E,PIERRE F,DRUESNE Pecollo N,et al.Nitrites and nitrates from food additives and cancer risk:Results from the NutriNet-Santé cohort[J].European Journal of Public Health,2021,31(S3):ckab165.244.
[2] WINTER G,PEREG L.A review on the relation between soil and mycotoxins:Effect of aflatoxin on field,food andfinance[J].European Journal of Soil Science,2019,70(4):882-897.
[3] 米彦飞.畜产品兽药残留危害及检测方法[J].畜牧兽医科学(电子版),2021(15):176-177.MI Yan-fei.Hazard and detection method of veterinary drug residues in animal products[J].Animal Science and Veterinary Science(Electronic),2021(15):176-177.
[4] LI Qi,SONG Pan,WEN Jiang-wen.Melamineand food safety:A 10-year review[J].Current Opinion in Food Science,2019,30:79-84.
[5] QU Ling-li,YU Huan,YIN Shuo,et al.Solid-phase extraction combined with ultra-high-performance liquid chromatography-tandem mass spectrometry for the determination of 5 trace nitro-polycyclic aromatic hydrocarbons in barbecued foods[J].Journal of Aoac International,2020,103(6):1 512-1 520.
[6] 易守福,梁锋,何青科,等.QuEChERS-气相色谱—三重四极杆质谱法同时测定黑茶中10种酰胺类除草剂残留[J].食品与机械,2021,37(8):57-62.YI Shou-fu,LIANG Feng,HE Qing-ke.Simultaneous determination of 10 kinds of amide herbicide residues in dark tea by quechers with gas chromatography-triple quadrupole mass spectrometry[J].Food & Machinery,2021,37(8):57-62.
[7] 刘正富,敖云胜,孙东红,等.液相色谱—串联质谱技术在食品安全检测中的应用[J].中国食品,2022(4):135-137.LIU Zheng-fu,AO Yun-sheng,SUN Dong-hong,et al.Application of liquid chromatography-tandem mass spectrometry in food safety detection[J].China Food,2022(4):135-137.
[8] 林津,范小龙,江丰,等.固相萃取—高效液相色谱—串联质谱法测定动物源食品中万古霉素类抗生素残留量[J].食品与机械,2020,36(9):67-71.LIN Jin,FAN Xiao-long,JIANG Feng,et al.Determination of vancomycin antibiotics residues in animal derived food by solid phase extraction and high performance liquid chromatography-tandem mass spectrometry[J].Food & Machinery,2020,36(9):67-71.
[9] WANG Feng,WAN De-bin,SHEN Yuan-dong,et al.Development of a chemiluminescence immunoassay for detection of tenuazonic acid mycotoxin in fruit juices with a specific camel polyclonal antibody[J].Analytical Methods,2021,13(15):1 795-1 802.
[10] 王宝新,张爱萍.化学发光免疫方法在食品安全检测中的应用及展望[J].中国食品工业,2021(18):61-63.WANG Bao-xin,ZHANG Ai-ping.Application and prospect of chemiluminescence immunoassay in food safety detection[J].China Food Industry,2021(18):61-63.
[11] REMFRY S E,AMACHAWADI R G,ATOBATELE M,et al.Shiga toxin-producing escherichia coli in wheat grains:Detection and isolation by polymerase chain reaction and culture methods[J].Foodborne Pathogens and Disease,2021,18(10):752-760.
[12] 曲识.食品沙门氏菌检测中PCR技术的应用分析[J].航空航天医学杂志,2021,32(10):1 230-1 233.QU Shi.Application and analysis of PCR technology in food salmonella detection[J].Journal of Aerospace Medicine,2021,32(10):1 230-1 233.
[13] 廖小艳,陈丽丽,白亚龙.食品中诺如病毒检测技术研究进展[J].食品与机械,2021,37(4):200-206.LIAO Xiao-yan,CHEN Li-li,BAI Ya-long.The progress of detection methods for norovirus in foods[J].Food & Machinery,2021,37(4):200-206.
[14] TU Chun-yan,DAI Yuan-yuan,ZHANG Ying,et al.A simple fluorescent strategy based on triple-helix molecular switch for sensitive detection of chloramphenicol[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2020,224:117415.
[15] HE Hui,XIE Chu-chu,YAO Liu,et al.A sensitive fluorescent assay for tetracycline detection based on triple-helix aptamer probe and cyclodextrin supramolecular inclusion[J].Journal of Fluorescence,2021,31(1):63-71.
[16] QI Xiao-yan,YAN Xiao-chen,ZHAO Lian-hui,et al.A facile label-free electrochemical aptasensorconstructed with nanotetrahedron and aptamer-triplex for sensitive detection of small molecule:Saxitoxin[J].Journal of Electroanalytical Chemistry,2020,858:113805.
[17] HU Yu-wei,CECCONELLO A,IDILI A,et al.Triplex DNA nanostructures:From basic properties to applications[J].Angewandte Chemie International Edition,2017,56(48):15 210-15 233.
[18] CHEN Jin,TANG Qing-nan,GUO Shi-wen,et al.Parallel triplex structure formed between stretched single-stranded DNA and homologous duplex DNA[J].Nucleic Acids Research,2017,45(17):10 032-10 041.
[19] BHUMA N,TÄHTINEN V,VIRTA P.Synthesis and applicability of base-discriminating DNA-triplex-forming 19FNMR probe[J].European Journal of Organic Chemistry,2018,2 018(5):605-613.
[20] KUMAR V,BRODYAGING N,ROZNERS E.Triplex-forming peptide nucleic acids with extended backbones[J].Nucleic Acids Research,2020,21(23):3 410-3 416.
[21] SU Yang-dong,BAYARJARGAL M,HALE T K,et al.DNA with zwitterionic and negatively charged phosphate modifications:Formation of DNA triplexes,duplexes and cell uptake studies[J].Beilstein Journal of Organic Chemistry,2021,17:749-761.
[22] GUO Yi-jun,YAO Dong-bao,ZHENG Bin,et al.pH-controlled detachable DNA circuitry and its application in resettable self-assembly of spherical nucleic acids[J].ACS Nano,2020,14(7):8 317-8 327.
[23] OLIVEIRA L M,LONG A S,BROWN T,et al.Melting temperature measurement and mesoscopic evaluation of single,double and triple DNA mismatches[J].Chemical Science,2020,11(31):8 273-8 287.
[24] 杜宇丹.基于三链DNA分子开关的Aptamer传感方法构建与应用研究[D].长沙:湖南大学,2014:16.DU Yu-dan.Study on the construction and application of aptamer sensing methods based on the triplex-stranded DNA molecular switch[D].Changsha:Hunan University,2014:16.
[25] ANN J C,MICHAEL W,VAN D.Oligodeoxyribonucleotide length and sequence effects on intermolecular purine purine pyrimidine triple-helix formation[J].Nucleic Acids Research,1994,22(22):4 742-4 747.
[26] KUNKLER C N,HULEWICZ J P,HICKMAN S C,et al.Stability of an RNA*DNA-DNA triple helix depends on base triplet composition and length of the RNA third strand[J].Nucleic Acids Research,2019,47(14):7 213-7 222.
[27] MARIOTTINI D,IDILI A,NIJENHUIS M A D,et al.Entropy-based rational modulation of the pKa of asynthetic pH-dependent nanoswitch[J].Journal of the American Chemical Society,2019,141(29):11 367-11 371.
[28] LI Xia,SONG Juan,XUE Qing-wang,et al.Sensitive and selective detection of the p53 gene based on a triple-helix magnetic probe coupled to a fluorescent liposome hybridization assembly via rolling circle amplification[J].Analyst,2017,142(19):3 598-3 604.
[29] HU Ying-xin,WANG Zhi-yu,CHEN Zhe-kun,et al.Switching the activity of taq polymerase using clamp-like triplex aptamer structure[J].Nucleic Acids Research,2020,48(15):8 591-8 600.
[30] IDILIA A,VALLEE Belisle A,RICCI F.Programmable pH-triggered DNA nanoswitches[J].Journal of the American Chemical Society,2014,136(16):5 836-5 839.
[31] CARR C E,GANUGULA R,SHIKIYA R,et al.Effect of dC→d(m5C)substitutions on the folding of intramolecular triplexes with mixed TAT and C+GC base triplets[J].Biochimie,2018,146:156-165.
[32] COLOCCI N,DERVAN P B.Cooperative bunding of 8-mer oligonucleotide containing 5-(1-propyny)-2'-deoxyuridine to adjacent DNA sites by triple-helix formation[J].Journal of the American Chemical Society,1994,116(2):785-786.
[33] MIAO Shi-qin,LIANG Yu-feng,MARATHE I,et al.Duplex stem replacement with bPNA+ triplex hybrid stems enables reporting on tertiary interactions of internal RNA domains[J].Journal of the American Chemical Society,2019,141(23):9 365-9 372.
[34] CHEN Feng,LU Qiu-jun,HUANG Lin-na,et al.DNA triplex and quadruplex assembled nanosensors for correlating K+ and pH in lysosomes[J].Angewandte Chemie International Edition,2021,60(10):5 453-5 458.
[35] WALSH S,ELS A H,BROWN T.Fluorogenic thiazole orange TOTFO probes stabilise parallel DNA triplexes at pH 7 and above[J].Chemical Science,2018,9(39):7 681-7 687.
[36] YAMAYOSHI A,MIYOSHI D,ZOUZUMI Y K,et al.Selective and robust stabilization of triplex DNA structures using cationic comb-type copolymers[J].The Journal of Physical Chemistry B,2017,121(16):4 015-4 022.
[37] ZHANG Zhong-liang,WU Yuan-yuan,XI Kun,et al.Divalent ion-mediated DNA-DNA interactions:A comparative study of triplex and duplex[J].Biophysical Journal,2017,113(3):517-528.
[38] XI Kun,WANG Feng-hua,XIONG Gui,et al.Competitive binding of Mg2+ and Na+ ions to nucleic acids:From helices to tertiary structures[J].Biophysical Journal,2018,114(8):1 776-1 790.
[39] SINGLETON S F,DERVAN P B.Equilibrium association constants for oligonucleotide-directed triple helix formation at single DNA sites:Linkage to cation valence and concentration[J].Biochemistry,1993,32(48):13 171-13 179.
[40] 杨晓广.超高效液相色谱—串联质谱在食品农药残留和兽药残留及添加剂检测分析中的应用[J].食品安全导刊,2021(12):171-172.YANG Xiao-guang.Application of ultra-performance liquid chromatography-tandem mass spectrometry in the detection and analysis of pesticide residues and veterinary drug residues and additives in food[J].Food Safety Guide,2021(12):171-172.
[41] LI Yu-bin,WANG Lei,ZHAO Li-ting,et al.An fluorescence resonance energy transfer sensing platform based on signal amplification strategy of hybridization chain reaction and triplex DNA for the detection of Chloramphenicol in milk[J].Food Chemistry,2021,357:129769.
[42] ZHANG Yong,DU Xiao-sheng,DENG Sha,et al.Dual triple helix-aptamer probes for mix-and-read detecting antibiotics in fish and milk[J].Journal of Agricultural and Food Chemistry,2020,68(35):9 524-9 529.
[43] TANG Xiao-min,LI Xiao-ton,MA D L,et al.A label-free triplex-to-G-qadruplex molecular switch for sensitive fluorescent detection of acetamiprid[J].Talanta,2018,189:599-605.
[44] LIU Xiao-juan,XU Ning-ning,GAI Pan-pan,et al.Triplex DNA formation-mediated strand displacement reaction for highly sensitive fluorescent detection of melamine[J].Talanta,2018,185:352-358.
[45] YANG Yu-cai,HUANG Yue,LI Chao.A reusable electrochemical sensor for one-step biosensing in complex media using triplex-forming oligonucleotide coupled DNA nanostructure[J].Analytica Chimica Acta,2019,1 055:90-97.
[46] ZHONG Miao,YANG Sheng-yuan,CHEN Lei,et al.Highly sensitive and efficient fluorescent sensing for Hg2+ detection based on triple-helix molecular switch and exonuclease III-assisted amplification[J].Analytica Chimica Acta,2022,1 205:339751.
[47] XU Lin,DA Xiu,JIANG Bing-ying,et al.Melamine-mediated base mismatch for label-free and amplified sensitive fluorescent detection of melamine in milk[J].Food Analytical Methods,2019,12(5):1 255-1 261.
[48] DUTTA D,KAUSHIK A,KUMAR D,et al.Foodborne pathogenic vibrios:Antimicrobial resistance[J].Frontiers in Microbiology,2021,12:638331.
[49] CAI Rong-feng,ZHANG Zhong-wen,CHEN Hao-han,et al.A versatile signal-on electrochemical biosensor for Staphylococcus aureus based on triple-helix molecular switch[J].Sensors and Actuators B:Chemical,2021,326:128842.
[50] GAO Xiao-shan,LI Hong-kun,ZHAO Yu,et al.Triple-helix molecular switch-based versatile “off-on” electrochemiluminescence and fluorescence biosensing platform for ultrasensitive detection of lipopolysaccharide by multiple-amplification strategy[J].Biosensors and Bioelectronics,2019,143:111602.
[51] LIU Xiao-juan,SONG Meng-meng,LI Feng.Triplex DNA-based bioanalytical platform for highly sensitive homogeneous electrochemical detection of melamine[J].Scientific Reports,2017,7(1):4 490.
[52] 孙熠,高翠.食品中真菌毒素的仪器分析技术研究[J].食品安全导刊,2021(8):68-69.SUN Yi,GAO Cui.Instrumental analysis of mycotoxins in food[J].Food Safety Guide,2021(8):68-69.
[53] HE Kai-yu,SUN Li-ping,WANG Liu,et al.Engineering DNA G-quadruplex assembly for label-free detection of Ochratoxin A in colorimetric and fluorescent dual modes[J].Journal of Hazardous Materials,2022,423:126962.
[54] XU Wen-tao,TIAN Jing-jing,SHAO Xiang-li,et al.A rapid and visual aptasensor for Lipopolysaccharides detection based on the bulb-like triplex turn-on switch coupled with HCR-HRP nanostructures[J].Biosensors and Bioelectronics,2017,89:795-801.
[55] WANG Jing,LI Hai-gang,LI Ting-ting,et al.Determination of bacterial DNA based on catalytic oxidation of cysteine by G-quadruplex DNAzyme generated from asymmetric PCR:Application to the colorimetricdetection of staphylococcus aureus[J].Microchimica Acta,2018,185(9):410.
[56] XU Li-jun,GUO Ya-hui,WANG Ji-ne,et al.A H+/Ag+ dual-target responsive label-free light-up probe based on a DNA triplex[J].Chemistry an Asian Journal,2015,10(5):1 126-1 129.
[57] VERDIAN A,ROUHBAKHSH Z,FOOLADI E.An ultrasensitive platform for PCB77 detection:New strategy for liquid crystal-based aptasensor fabrication[J].Journal of Hazardous Materials,2021,402:123531.

Included in

Food Science Commons



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.