Objective: In this study, the effects of exogenous additives on gel properties of myofibrillar protein were explored to improve the property of surimi from fresh-water fish. Methods: The myofibrillar protein of loach was mixed with various contents of soybean protein isolate and carrageenan to prepare composite gel. Its properties were investigated by texture analyzer, color difference analyzer, scanning electron microscope and infrared spectroscopy. Results: With increased content of soybean protein isolate, the gel hardness of myofibrillar protein from loach decreased firstly and then increased; while gel springiness increased firstly and then decreased. Soybean protein isolate could enhance the water holding capacity of gel and decrease its cooking loss. Holes in gel network became larger and the relative contents of secondary structure also changed. However, carrageenan could weaken the hardness and springiness of gel, while improve the whiteness as well as cooking loss. The gel network structure became regular and the unordered structure disappeared. Conclusion: The effects of soybean protein isolate on gel of myofibrillar protein from loach is more obvious than that of carrageenan, and the composite gel formed by soybean protein isolate have better properties.

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[1] SUN P P,REN Y Y,WANG S Y,et al.Characterization and film-forming properties of acid soluble collagens from different by-products of loach(Misgurnus anguillicaudatus)[J].LWT-Food Science and Technology,2021,149:111844.
[2] 徐乐俊,吕永辉,高宏泉,等.中国渔业统计年鉴:2018[M].北京:中国农业出版社,2018:25.XU L J,LU Y H,GAO H Q,et al.China fishery statistical yearbook:2018[M].Beijing:China Agriculture Press,2018:25.
[3] 袁晓初,赵文武,高宏泉,等.中国渔业统计年鉴:2016[M].北京:中国农业出版社,2016:31.YUAN X C,ZHAO W W,GAO H Q,et al.China fishery statistical yearbook:2016[M].Beijing:China Agriculture Press,2016:31.
[4] 董金和,陈恩友,袁晓初,等.中国渔业统计年鉴:2014[M].北京:中国农业出版社,2014:31.DONG J H,CHEN E Y,YUAN X C,et al.China fishery statistical yearbook:2014[M].Beijing:China Agriculture Press,2014:31.
[5] 王静宇,胡新,刘晓艳,等.肌原纤维蛋白热诱导凝胶特性及化学作用力研究进展[J].食品与发酵工业,2020,46(8):300-306.WANG J Y,HU X,LIU X Y,et al.Advances in heat-induced gel properties and chemical forces of myofibrillar protein gel[J].Food and Fermentation Industries,2020,46(8):300-306.
[6] 胡娟.脉冲电场对肌原纤维蛋白结构、乳化及凝胶特性的影响[D].扬州:扬州大学,2021:1-5.HU J.Effect of pulsed electric field on the structure,emulsification and gel properties of myofibrillar protein[D].Yangzhou:Yangzhou University,2021:1-5.
[7] 吕旭琴,周娇娇,蔡杰,等.基于多糖调节肌原纤维蛋白凝胶特性的研究进展[J/OL].食品工业科技.(2022-01-17)[2022-03-23].https://doi.org/10.13386/j.issn1002-0306.2021100012.LU X Q,ZHOU J J,CAI J,et al.Research progress in regulation of the gel properties of myofibrillar protein based on polysaccharides[J/OL].Science and Technology of Food Industry.(2022-01-17)[2022-03-23].https://doi.org/10.13386/j.issn1002-0306.2021100012.
[8] LIN D Q,ZHANG L T,LI R J,et al.Effect of plant protein mixtures on the microstructure and rheological properties of myofibrillar protein gel derived from red sea bream(Pagrosomus major)[J].Food Hydrocolloids,2019,96:537-545.
[9] PAN S,WU S.Effect of chitooligosaccharides on the denaturation of weever myofibrillar protein during frozen storage[J].International Journal of Biological Macromolecules,2014,65(5):549-552.
[10] URBONAITE V,DE JONGH H H J,VAN DER LINDEN E,et al.Water holding of soy protein gels is set by coarseness,modulated by calcium binding,rather than gel stiffness[J].Food Hydrocolloids,2015,46:103-111.
[11] BYLER D M,SUSI H.Examination of the secondary structure of proteins by deconvolved FTIR spectra[J].Biopolymers,1986,25(3):469-487.
[12] 赵泽润,邢通,赵雪,等.大豆分离蛋白添加量对低盐木质化鸡肉糜凝胶特性的影响[J].食品科学,2022,43(6):49-56.ZHAO Z R,XING T,ZHAO X,et al.Effects of soy protein isolate content on the gelation properties of low-salt wooden chicken breast meat paste[J].Food Science,2022,43(6):49-56.
[13] 孙泽坤,谢云飞,于航,等.魔芋胶与卡拉胶复配优化驴头骨肉冻凝胶特性[J].食品与发酵工业,2022,48(1):146-152.SUN Z K,XIE Y F,YU H,et al.Optimization of meat jelly properties from donkey skull by konjac gum/carrageenan mixtures[J].Food and Fermentation Industries,2022,48(1):146-152.
[14] NIU H L,XIA X F,WANG C,et al.Thermal stability and gel quality of myofibrillar protein as affected by soy protein isolates subjected to an acidic pH and mild heating[J].Food Chemistry,2017,242:188-195.
[15] LI J G,CHEN Y H,DONG X P,et al.Effect of chickpea(Cicer arietinum L.)protein isolate on the heat-induced gelation properties of pork myofibrillar protein[J].Journal Science of Food and Agriculture,2020,101(5):2 108-2 116.
[16] 张钰嘉,万杨卓群,石尚轩,等.磷酸盐—大豆分离蛋白联合处理对草鱼肌原纤维蛋白凝胶化的影响[J].肉类研究,2022,36(2):21-26.ZHANG Y J,WAN Y Z Q,SHI S X,et al.Effects of combined treatment of phosphates and soybean protein isolate on the gelation properties of myofibrillar proteins from grass carp[J].Meat Research,2022,36(2):21-26.
[17] 张立彦,胡嘉颖,王仕钰.食盐添加量对微波加热鸡胸肉糜凝胶品质的影响[J].现代食品科技,2016,32(3):183-188.ZHANG L Y,HU J Y,WANG S Y.Effects of the amount of added salt on gel properties of microwave-heated chicken breast mince[J].Modern Food Science and Technology,2016,32(3):183-188.
[18] 张蒙琪.鸡蛋蛋白与大豆分离蛋白间相互作用及其凝胶特性的研究[D].无锡:江南大学,2021:26-38.ZHANG M Q.The research of interaction between hen egg proteins and soybean protein isolate and its gelation properties[D].Wuxi:Jiangnan University,2021:26-38.
[19] 胡爱军,于辉,郑婕,等.木薯淀粉对泥鳅肌原纤维蛋白凝胶特性的影响[J].天津科技大学学报,2020,35(4):21-30.HU A J,YU H,ZHENG J,et al.Effect of cassava starch on gel properties of loach myofibrillar protein[J].Journal of Tianjin University of Science & Technology,2020,35(4):21-30.
[20] 叶贝贝.白鲢鱼/金线鱼混合肌原纤维蛋白及肌球蛋白热聚集的作用机制[D].锦州:渤海大学,2019:22-23.YE B B.Mechanism of thermal aggregation of mixed myofibrillar protein and myosin from Hypophthalmichtys molitrix and Nemipterus virgatus[D].Jinzhou:Bohai University,2021:22-23.
[21] 张倩.大豆蛋白/多糖复合凝胶缓释体系构建与表征[D].无锡:江南大学,2021:35-36.ZHANG Q.Preparation and characterization of soybean protein/polysaccharide composite gels slow-release system[D].Wuxi:Jiangnan University,2021:35-36.
[22] 汤嘉惠.κ-卡拉胶/K+凝胶体系对鱼糜性质和结构的影响[D].上海:上海海洋大学,2020:31-32.TANG J H.Effects of κ-carrageenan/K+ gel system on the properties and structure of surimi[D].Shanghai:Shanghai Ocean University,2020:31-32.
[23] 梁恽红,卢涵,张香美.蛋白二、三级结构对鱼糜凝胶质构和持水力的影响及其测定方法研究进展[J].东北农业大学学报,2021,52(10):87-96.LIANG Y H,LU H,ZHANG X M.Research progress on the effects of protein secondary and tertiary structures on texture and water-holding capacity of surimi gel and protein structure determination methods[J].Journal of Northeast Agricultural University,2021,52(10):87-96.

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