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Abstract

To study the antimicrobial potential of vapor-phase Eucalyptus essential oil, the antimicrobial activity and minimum inhibitory concentrations (MIC) were evaluated against the tested microorganisms in vapor, liquid and solid phases. The chemical constitutes of Eucalyptus essential oil and its vapor were analyzed by gas chromatography-mass spectrometry (GC-MS) and solid phase microextraction (SPME) GC-MS. The results demonstrated that Eucalyptus essential oil vapor had obvious antimicrobial effect on the tested microorganisms. The antimicrobial activities of Eucalyptus essential oil vapor against Escherichia coli and Staphylococcus aurous were stronger than that against Aspergillus niger. The MIC value (0.045~0.36 mg/mL) in the vapor phase to the tested microorganisms was significantly lower than that in the liquid-phase MIC (4.5~18.0 mg/mL) and the solid phase MIC (4.5~18.0 mg/mL). The dominant components, mainly terpenes and alcohols, showed not significant difference between the Eucalyptus essential oil and its vapor. The antibacterial activity of Eucalyptus essential oil vapor was related to α-pinene, D-Limonene, 3-Carene, Terpinolene and Myrcene, and the relative increase in terpene contents led to the stronger antibacterial activity.

Publication Date

12-28-2017

First Page

70

Last Page

75

DOI

10.13652/j.issn.1003-5788.2017.12.014

References

[1] GOLDBECK J C, NASCIMENTO J E, JACOB R C, et al. Bioactivity of essential oils from Eucalyptus globulus and Eucalyptus urograndis against planktonic cells and biofilms of Streptococcus mutans[J]. Industrial Crops and Products, 2014, 60: 304-309.
[2] 黄承标, 杨钙仁, 魏国余, 等. 按树林地枯枝落叶层的水文特性及养分贮量[J]. 福建林学院学报, 2011, 31(4): 289-294.
[3] 陈默, 余永莉. 桉叶油的化学成分及其生物活性研究进展[J]. 中国现代医药杂志, 2014(4): 97-100.
[4] ANNE K T, AYYAZ M. Antimicrobial potential and chemical composition of Eucalyptus globules oil in liquid and vapour phase against food spoilage microorganisms[J]. Food Control, 2011, 22(22): 1 707-1 714.
[5] GOLDBECK J C, NASCIMENTO J E, JACOB R G, et al. Bioactivity of essential oils from Eucalyptus globulus and Eucalyptus urograndis against planktonic cells and biofilms of Streptococcus mutans Malik A[J]. Ind. Crop. Prod., 2014, 60: 304-309.
[6] 王挥, 龚吉军, 唐静, 等. 肉桂与桉叶复合精油处理对“夏黑”葡萄保鲜质量的影响[J]. 食品安全质量检测学报, 2016, 7(9): 3 703-3 709.
[7] BATISH D R, SINGH H P, KOHLI R K. Eucalyptus essential oil as a natural pesticide[J]. Forest Ecol. Manag., 2008, 256(12): 2 166-2 174.
[8] 周春禾, 王建清, 金政伟. 尤加利精油制备微胶囊抗菌纤维素薄膜[J]. 包装工程, 2011, 32(1): 40-42.
[9] 任小玲, 岳淑丽, 向红, 等. 桉叶精油微胶囊工艺优化及缓释性能研究[J]. 包装工程, 2017, 38(9): 107-112.
[10] GILLESM, ZHAO J, AN M, et al. Chemical composition and antimicrobial properties of essential oils of three Australian Eucalyptus species[J]. Food Chemistry, 2010, 119(2): 731-737.
[11] OLOLADE Z S, OLAWORE N O, OLASOJI S O, et al. Chemical composition and bactericidal activities of the leaf essential oil of eucalyptus maculata hook[J]. Natural Product Chemistry Research, 2017, 5(2): 257-260.
[12] 周汉军. 桉叶精油提取、纳米乳化及其抑菌活性研究[D]. 长沙: 中南林业科技大学, 2016: 3-4, 21-23.
[13] 刘伟. 蓝桉叶精油微波无溶剂法提取及抗氧化、抗菌活性研究[D]. 哈尔滨: 东北林业大学, 2015: 25-28.
[14] 童迅, 高雯, 黄庭轩, 等. 浓缩果汁中耐热霉菌的分析及鉴定[J]. 食品科学, 2016, 37(20): 198-202.
[15] 刘晓丽, 钟少枢, 吴克刚, 等. 丁香和肉桂精油气相抑菌活性研究[J]. 食品与发酵工业, 2010, 36(1): 21-24, 38.
[16] LPEZ P, SANCHEZ C, BATLLE R, et al. Vapor-phase activities of cinnamon, thyme, and oregano essential oils and key constituents against foodborne microorganisms[J]. J. Agric. Food Chem., 2007, 55 (11): 4 348-4 356.
[17] 岳淑丽, 任小玲, 陈霞, 等. 桉叶精油包埋前后抑菌性能及成分比较研究[J]. 食品科学, 2017, 38(11): 155-160.
[18] 刘晓丽, 吴克刚, 柴向华, 等. 肉桂精油对储粮霉菌的抑制作用研究[J]. 安徽农业科学, 2012, 40(27): 13 570-13 572.
[19] TYAGI A K, MALIK A. Antimicrobial potential and chemical composition of Eucalyptus globulus oil in liquid and vapour phrase against food spoilage microorganisms[J]. Food Chem., 2011, 126: 228-235.
[20] 蓝亮美, 马丽, 郭占京, 等. 三种桂产桉叶油对常见致腐菌的抑制作用研究[J]. 食品工业科技, 2014, 35(22): 155-158.
[21] 郭俊贤, 刘晓丽, 吴克刚, 等. 11种精油对三种水产品特定腐败菌的抑制[J]. 中国食品添加剂, 2014(6): 54-57.
[22] 杨忠全, 阚建全, 陈光静, 等. 新鲜薏仁米与异味薏仁米挥发性成分对比分析[J]. 食品与机械, 2017, 33(7): 35-39.
[23] ROTA M C, HERRERA A, MARTINEZ R M, et al. Antimicrobial activity and chemical composition of Thymus vulgar is, Thymuszyg-is and Thymus hyemal is essential oils[J]. Food Control, 2008, 19(7): 681-687.
[24] 刘光发, 王建清, 赵亚珠. 牛至精油微胶囊的制备及其抑菌效果研究[J]. 包装工程, 2012, 33(3): 19-22.
[25] LPEZ P, SANCHEZ C, BATLLE R, et al. Solid-and vapor-phase antimicrobial activities of six essential oils: susceptibility of selected foodborne bacterial and fungal strains[J]. J. Agric. Food Chem., 2005(53): 6 939-6 946.
[26] 田玉红, 周琪, 颇俊华, 等. 广林九号桉叶精油的抑菌活性研究[J]. 湖北农业科学, 2013(21): 5 193-5 196.
[27] KUMAR P, MISHRA S, MALIK A, et al. Compositional analysis and insecticidal activity of Eucalyptus globules (family: Myrtaceae) essential oil against housefly (Musca domestica)[J]. Acta. Trop., 2012, 122(2): 212-218.
[28] 叶舟. 大叶桉叶精油化学成分及其抑菌活性[J]. 福建林学院学报, 2007, 27(1): 48-51.
[29] AIT-OUAZZOU A, LORN S, BAKKALI M, et al. Chemical composition and antimicrobial activity of essential oils of Thymus algeriensis, Eucalyptus globulus and Rosmarinus officinalis from Morocco[J]. J. Sci. Food Agric., 2011, 91(14): 2 643-2 651.
[30] BAKKALI F, AVERBECK S, AVERBECK D, et al. Biological effects of essential oils: a review[J]. Food Chem. Toxicol., 2008, 46(2): 446-475.
[31] SPEROTTO A R M, MOURA D J, PRES V F, et al. Cytotoxic mechanism of Piper gaudichaudianum Kunth essential oil and its major compound nerolidol[J]. Food Chem. Toxicol., 2013, 57(12): 57-68.
[32] TYAGI A K, MALIK A. Antimicrobial potential and chemical composition of Mentha piperita oil in liquid and vapour phase against food spoiling microorganisms[J]. Food Control, 2011, 22 (11): 1 707-1 714.
[33] TYAGI A K, MALIK A. In situ SEM, TEM and AEM studies of the antimicrobial activity of lemon grass oil in liquid and vapour phase against Candida albicans[J]. Micron., 2010, 41(7): 797-805.
[34] LEOPOLO J, GERHARD B, ALBERA S, et al. Composition quality control and antimicrobial activity of the essential oil of long time dill seeds from Bulgaria[J]. Chem. Nat. Compd., 2003, 51(2): 3 854-3 857.
[35] 夏忠弟, 毛学政, 罗映辉. α-蒎烯抗真菌机制的研究[J]. 湖南医科大学学报, 1999(6): 507-509.
[36] 郝静梅, 盛冉, 孙志高, 等. 柠檬烯抗菌性研究进展[J]. 食品与发酵工业, 2017, 43(2): 274-278.
[37] 关天旺, 刘嘉煜. 柠檬烯的防腐作用及抑菌机理研究进展[J]. 保鲜与加工, 2015(6): 83-87.
[38] 何丽芝, 王婧, 赵振东, 等. 3-蒈烯资源及其生物活性应用研究进展[J]. 林产化学与工业, 2011, 31(3): 122-126.

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