Research on antimicrobial and antioxidant activities of major components of lemon peel essential oil
Abstract
Antioxidant and antibacterial activities in vitro of three major components dl-limonene, γ-terpinene and α-terpineol contained in Eureka lemon peel essential oil as well as its compound were explored in mainly in the determination of DPPH free radical as well as OH· free radical scavenging ability and its bacteriostasis size and MIC against Escherichia coli, Bacillus subtilis and Staphylococcus aureus of the three major components. Results showed that dl-limonene, γ-terpinene, α-terpineol had certain scavenging effect on OH· free radical with clearance rate between 48.45%～77.94%; and the scavenging effect of DPPH free radical was higher than that of LEO at the same concentration. Antibacterial activity test results indicated that dl-limonene, γ-terpinene, α-terpineol showed certain inhibitory effects against the three tested bacteria and lower than that of LEO at the same concentration, α-terpineol showed a best antibacterial effect on Escherichia coli and Bacillus subtilis with MIC 3.9 μL/mL and 1.0 μL/mL respectively, while dl-limonene had better antibacterial effect on Staphylococcus aureus with MIC 1.0 μL/mL; compound composed of dl-limonene, γ-terpinene, α-terpineol showed better antimicrobial effect than monomers. Overall experiment results showed that dl-limonene, γ-terpinene and α-terpineol had certain influence on LEO’s antioxidant and antibacterial activity.
Publication Date
12-28-2017
First Page
138
Last Page
142
DOI
10.13652/j.issn.1003-5788.2017.12.028
Recommended Citation
Bin, ZHANG; Xiaozhen, HOU; Yi, QIN; Xin, DING; Qihai, DENG; and Linjie, HUANG
(2017)
"Research on antimicrobial and antioxidant activities of major components of lemon peel essential oil,"
Food and Machinery: Vol. 33:
Iss.
12, Article 28.
DOI: 10.13652/j.issn.1003-5788.2017.12.028
Available at:
https://www.ifoodmm.cn/journal/vol33/iss12/28
References
[1] YAGHMAEI P, PARIVAR K, SAVAR M H. Investigation on the effect of lemon peel essential oil in blood level lipids number of differential leukocytes of Wistar rats[J]. Journal of the Neurological Sciences, 2009, 283(1/2): 297-297.
[2] FISHER K, PHILLIPS C A. The effect of lemon, orange and bergamot essential oils and their components on the survival of Campylobacter jejuni, Escherichia coli O157, Listeria monocytogenes, Bacillus cereus and Staphylococcus aureus in vitro and in food systems[J]. Journal of Applied Microbiology, 2006, 101(6): 1 232-1 240.
[3] 章斌, 侯小桢, 秦轶, 等. 柠檬果皮精油的化学组成、抗氧化及抑菌活性研究[J]. 食品工业科技, 2015, 36(5): 126-131.
[4] ZAHI R Z, HATTAB M.E., LIANG Hao, et al. Enhancing the antimicrobial activity of d-limonene nanoemulsion with the inclusion of ε-polylysine[J]. Food Chemistry, 2017, 221: 18-23.
[5] LOKU U A, BECERRA N, KOHLI P, et al. Antimicrobial efficacy of liposomes containing d-limonene and its effect on the storage life of blueberries[J]. Postharvest Biology and Technology, 2017, 128: 130-137.
[6] KESKIN I, GUNAL Y, AYLA S, et al. Effects of Foeniculum vulgare essential oil compounds, fenchone and limonene, on experimental wound healing[J].Biotechnic and Histochemistry, 2017, 92(4): 274-282.
[7] MATHEUS D Baldissera, THIRSSA H Grando, CARINE F Souza, et al. Invitro and invivo action of terpinen-4-ol, γ-terpinene, and α-terpinene against Trypanosoma evansi[J]. Experimental Parasitology, 2016, 162: 43-48.
[8] RUDBUCK J, BERGSTRM M A, BURJE A, et al. α-Terpinene, an antioxidant in tea tree oil, autoxidizes rapidly to skin allergens on air exposure[J]. Chemical Research in Toxicology, 2012, 25(3): 713-721.
[9] WU Zi-li, YIN Zhong-qiong, DU Yong-hua, et al. γ-terpineol inhibits cell growth and induces apoptosis in human liver cancer BEL-7402 cells in vitro[J]. International Journal of Clinical and Experimental Pathology, 2015, 17(22): 5 686-5 689.
[10] PRIYANKA S, RAVINDRA S, BHANU P, et al. Chemical profile, antifungal, antiaflatoxigenic and antioxidant activity of Citrus maxima Burm. and Citrus sinensis (L.) Osbeck essential oils and their cyclic monoterpene, dl-limonene[J]. Food and Chemical Toxicology, 2010, 48(6): 1 734-1 740.
[11] MARTUCCI J F, GENDE L B, NEIRA L M, et al. Oregano and lavender essential oils as antioxidant and antimicrobial additives of biogenic gelatin films[J]. Industrial Crops and Prod-ucts, 2015, 71: 205-213.
[12] 李悦, 侯滨滨, 赵婧. 柑橘类精油抗菌活性的研究[J]. 食品研究与开发, 2011, 32(6): 190-192.
[13] 陈思佳, 刘雅丽, 张晨, 等. 柠檬精油抗氧化活性的研究[J]. 实用口腔医学杂志, 2015, 31(3): 343-346.
[14] 黄娜娜. 柑橘精油抗氧化特性及对皮肤细胞氧化损伤的保护作用研究[D]. 武汉: 华中农业大学, 2016.
[15] CHOI H, HEE S, HIROYUKI U, et al. Radical-scavenging activities of citrus essential oils and their components: Detection using 1,1-diphenyl-2-picrylhydrazyl[J]. Journal of Agricultural and Food Chemistry, 2000, 48(9): 4 156-4 161.
[16] MRIO R M J, THOMAZ A A R S, GILBERTO C F, et al. Antioxidant potential of aroma compounds obtained by lim-onene biotransformation of orange essential oil[J]. Food Chemistry, 2009, 116(1): 8-12.
[17] YONATHAN A, IKUKO T, SAYURI I, et al. Volatile aroma components and antioxidant activities of the flavedo peel extract of unripe shiikuwasha (Citrus depressa hayata)[J]. Journal of Food Science, 2012, 77(4): C469-C475.
[18] LI Guo-xiang, LIU Zai-qun. Unusual antioxidant behavior of α-and γ-terpinene in protecting methyl linoleate, DNA, and erythrocyte[J]. Journal of Agricultural and Food Chemistry, 2009, 57(9): 3 943-3 948.
[19] PIETRO L C, VELLASAMY S, NICOLA S I. Antibacterial activity of essential oil components and their potential use in seed disinfection[J]. Journal of Agricultural and Food Chemistry, 2009, 57(20): 9 454-9 461.
[20] NAL Mustafa mit, UAN Filiz, 瘙塁ENER Aysun, et al. Research on antifungal and inhibitory effects of DL-limonene on some yeasts[J].Turkish Journal of Agriculture and Forestry, 2012, 36(5): 576-582.
[21] MARINA D S, DEJAN D B, ANA M D, et al. Chemical composition and antifungal activity of Salvia desoleana Atzei & Picci essential oil and its major components[J]. Flavour and Fragrance Journal, 2009, 24(2): 83-87.
[22] AROSHA U, NATHALIE B, PUNIT K, et al. Antimicrobial efficacy of liposomes containing d-limonene and its effect on the storage life of blueberries[J]. Postharvest Biology and Technology, 2017, 128: 130-137.
[23] PARK S N, LIM Y K, FREIRE M O, et al. Antimicrobial effect of linalool and α-terpineol against periodontopathic and cariogenic bacteria[J]. Anaerobe, 2012, 18(3): 369-372.
[24] LI Hong, YANG Tian, LI Fei-yan, et al. Antibacterial activity and mechanism of action of Monarda punctata essential oil and its main components against common bacterial pathogens in respiratory tract[J]. International Journal of Clinical and Experimental Pathology, 2014, 7(11): 7 389-7 398.
[25] ANTONIO B, MARIA R, MILENA S. In vitro evaluation of the antimicrobial activity of eugenol, limonene, and citrus extract against bacteria and yeasts, representative of the spoiling microflora of fruit juices[J]. Journal of Food Protection, 2010, 73(5): 888-894.
[26] KEI S, SABINE K, GERHARD B. Antimicrobial effect of vapours of geraniol, linalool, terpineol, γ-terpinene and 1,8-cineole on airborne microbes using an airwasher[J]. Flavour and Fragrance Journal, 2007, 22(5): 435-437.
[27] EUGNIA P, MARIA G, PAULA P, et al. Activity of Thymus caespititius essential oil and α-terpineol against yeasts and filamentous fungi[J]. Industrial Crops & Products, 2014, 62: 107-112.
[28] PRIYANKA S, RAVINDRA S, BHANU P, et al. Chemical profile, antifungal, antiaflatoxigenic and antioxidant activity of Citrus maxima Burm. and Citrus sinensis (L.) Osbeck essential oils and their cyclic monoterpene, dl-limonene[J]. Food and Chemical Toxicology, 2010, 48(6): 1 734-1 740.