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Abstract

In this study, the optimum extraction parameters of polyphenols from leaves of Boehmeria nivea var. tenacissima were optimized, and the antioxidant activity of polyphenols from ramie leaves was evaluated by using gallic acid as a control. Results of polyphenols extraction experiment showed that the ultrasonic power and ultrasonic time have less effect on the amount of polyphenols extraction, and the influence of three factors on polyphenols extraction was ordered as: ethanol concentration (significantly)> extraction temperature > solid-liquid ratio. Finally, the optimized polyphenols extraction process parameters were verified and determined as: 1∶33 (g/mL) of liquid-solid ratio, 40% of ethanol concentration and 42 ℃ of the extraction temperature and the amount of polyphenols extraction could reach at 47.97 mg/g under this conditions. The results of antioxidant activity evaluation showed that polyphenols from leaves of green leaf ramie had good antioxidant effects, and the DPPH free radical scavenging ability [IC50 value (0.15±0.00) g/mL], ABTS free radical scavenging ability [IC50 value (2.28±0.03) g/mL] and oxygen free radical absorbing ability [ORAC value (42.41±0.01) g Trolox equivalent /g polyphenol] were significantly superior to gallic acid.

Publication Date

2-18-2023

First Page

152

Last Page

158

DOI

10.13652/j.issn.1003-5788.2020.12.032

References

[1] 韩丙军,彭黎旭.植物多酚提取技术及其开发应用现状[J].华南热带农业大学学报,2005,11(1):21-26.
[2] LI Tao,LI Qi-li,WU Wei-guo,et al.Lotus seed skin proanthocyanidin extract exhibits potent antioxidant property via activation of the Nrf2-ARE pathway[J].Acta Biochimica et Biophysica Sinica,2019,51(1):31-40.
[3] BAZYLKO A,PIWOWARSKI J P,FILIPEK A,et al.In vitro antioxidant and anti-inflammatory activities of extracts from Potentilla recta and its main ellagitannin,agrimoniin[J].Journal of Ethnopharmacology,2013,149(1):222-227.
[4] DAVIS D W,NAVALTA J W,MCGINNIS G R,et al.Effects of acute dietary polyphenols and post-meal physical activity on postprandial metabolism in adults with features of the metabolic syndrome[J].Nutrients,2020,12(4):1 120-1 143.
[5] 周媛,喻玲玲,李新兰,等.苎麻叶中总多酚的含量测定[J].食品科技,2009,34(4):193-197.
[6] 王满生,杨晶,王延周.青叶苎麻叶蛋白提取工艺的优化[J].食品工业,2020,41(7):70-73.
[7] 孙延炜,孟桂元,周静,等.苎麻近缘植物氨基酸组成及其营养评价[J].中国麻业科学,2013,35(6):296-299,328.
[8] 张雪蕾,张庆丽,陈青,等.纤维素酶对饲用苎麻青贮品质及饲用价值的影响[J].饲料研究,2018(6):33-37.
[9] 杨晶,戴求仲,侯振平,等.基于专利分析的麻类作物饲料化利用技术态势及热点分析[J].生物技术通报,2018,34(12):213-220.
[10] 王满生,杨晶,王延周,等.饲用苎麻与全株玉米混合青贮饲料的酸度分析[J].中国饲料,2018(13):78-82.
[11] 王满生,王延周,杨晶,等.饲用苎麻与玉米混合青贮饲料的气味分析[J].中国饲料,2018(5):75-79.
[12] 芮莹,徐庆,韦京辰,等.苎麻叶萃取物体外抗甲型流感病毒(H1N1)和抗氧化活性测定[J].中国药房,2019,30(8):1 061-1 066.
[13] 陈平,朱爱国,陈继康,等.超声波辅助浸提苎麻叶蛋白工艺优化技术[J].食品工业,2020,41(8):122-125.
[14] 林文秋,杨为海,邹明宏.澳洲坚果果皮不同溶剂提取物的含量和抗氧化活性[J].江苏农业科学,2017,45(1):171-174.
[15] 李曌,李宏强,陈国刚,等.溶剂类型对葡萄籽多酚提取及抗氧化活性的影响[J].食品工业,2016,37(12):122-127.
[16] HERRERA M C,LUQUE DE CASTRO M D.Ultrasound-assisted extraction for the analysis of phenolic compounds in strawberries[J].Analytical and Bioanalytical Chemistry,2004,379:1 106-1 112.
[17] 孙晨.微波辅助提取食品有效成分研究进展[J].粮食与油脂,2011(7):5-7.
[18] LAZZ M C,PIZZALA R,GUTIRREZ PECHARROM F J,et al.Grape waste extract obtained by supercritical fluid extraction contains bioactive antioxidant molecules and induces antiproliferative effects in human colon adenocarcinoma cells[J].Journal of Medicinal Food,20019,12(3):561-568.
[19] LUO Xiao-ping,CUI Jie-mei,ZHANG Hai-hui,et al.Subcritical water extraction of polyphenolic compounds from sorghum(Sorghum bicolor L.)bran and their biological activities[J].Food Chemistry,2018,262:14-20.
[20] ÁLVAREZ-CASAS M,GARCÍA-JARES C,LLOMPART M,et al.Effect of experimental parameters in the pressurized solvent extraction of polyphenolic compounds from white grape marc[J].Food Chemistry,2014,157:524-532.
[21] NADAR S S,RAO P,RATHOD V K.Enzyme assisted extraction of biomolecules as an approach to novel extraction technology:A review[J].Food Research International,2018,108:309-330.
[22] 许先猛,张增帅,郭俊花,等.苹果多酚提取和纯化关键技术研究进展[J/OL].食品与机械.(2020-10-27)[2020-11-06].https://kns.cnki.net/kcms/detail/43.1183.TS.20201027.1736.012.html.
[23] 贺银菊,张旋俊,杨再波,等.响应面优化紫果西番莲叶多酚超声辅助提取工艺及其抗氧化活性[J/OL].食品工业科技.(2020-06-24)[2020-11-06].https://kns.cnki.net/kcms/detail/11.1759.TS.20200624.1557.010.html.
[24] 蔡如玉,常世敏,宋春丽.“凤丹”油用牡丹籽多酚提取工艺优化及抗氧化活性研究[J].食品与机械,2020,36(7):181-186.
[25] 余欣珂,明建,支玲,等.真空冷冻干燥对菊花多酚含量及其抗氧化活性的影响[J].食品与机械,2020,36(6):138-144.
[26] 董怡.光果甘草叶黄酮的分离纯化、活性研究及应用[D].广州:华南理工大学,2016:31-45.
[27] CHEN Xiu-min,MU Kai-wen,KITTS D D.Characterization of phytochemical mixtures with inflammatory modulation potential from coffee leaves processed by green and black tea processing methods[J].Food Chemistry,2019,271(12):248-258.
[28] CHEN Xiu-min,MA Zhi-li,KITTS D D.Effects of processing method and age of leaves on phytochemical profiles and bioactivity of coffee leaves[J].Food Chemistry,2018,249(24):143-153.
[29] CHEN Xiu-min,KITTS D D,JI Da-yi,et al.Free radical scavenging activities of phytochemical mixtures and aqueous methanolic extracts recovered from processed coffee leaves[J].International Journal of Food Science and Technology,2019,54(10):2 872-2 879.

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