•  
  •  
 

Abstract

The extraction temperature, ratios of water to material and extraction time were taken as the influencing factors, and the extraction rate of polysaccharides from Clinacanthus nutans was taken as the evaluation index to carry out single factor experiment, and Box-Behnken response surface methodology was used to optimize the extraction process on the basis of single factor test results. The results showed that the best extraction conditions of polysaccharides from Clinacanthus nutans were as follows: the ratio of material to liquid was 1:31 (g/mL), the extraction temperature was 92 ℃, the extraction time was 1.6 h. and the extraction rate was 3.40%. The antioxidant activity test showed that the polysaccharide of Clinacanthus nutans had good scavenging ability in ABTS anion free radical and DPPH free radical and had certain scavenging ability on hydroxyl free radicals

Publication Date

2-28-2020

First Page

171

Last Page

175

DOI

10.13652/j.issn.1003-5788.2020.02.032

References

[1] NADARAJAN S,UMAR I M,DER-JIUN O,et al.Phenolic rich extract from Clinacanthus nutans,attenuates hyperlipidemia-associated oxidative stress in rats[J].Oxidative Medicine and Cellular Longevity,2016,2 016:1-16.
[2] TU Shu-fen,WU Yang-chang,CHENG Yuan-bin,et al.Chemical constituents and bioactivities of Clinacanthus nutans aerial parts[J].Molecules,2014,19(12):20 382-20 390.
[3] THONGRAKARD V,TENCOMNAO T.Modulatory effects of Thai medicinal plant extract on proinflammatory cytokines-induced apoptosis in human keratinocyte HaCaT cells[J].African Journal of Biotechnology,2010,9(31):4 999-5 003.
[4] LEE S Y,MEDIANI A,NUR ASHIKIN A H,et al.Antioxidant and α-glucosidase inhibitory activities of the leaf and stem of selected traditional medicinal plants[J].International Food Research Journal,2014,21(1):165-172.
[5] 段佳辉,徐德平,钱志娟,等.忧遁草抗氧化功效与成分的研究[J].食品科技,2019,44(3):205-210.
[6] HUANG Dan-min,GUO Wen-jie,GAO Jing,et al.Clinacanthus nutans(Burm.f.)Lindau ethanol extract inhibits hepatoma in mice through upregulation of the immune response[J].Molecules,2015,20(9):17 405-17 428.
[7] YOKE K Y,TAN Jun-jie,SOEK S T,et al.Clinacanthus nutans extracts are antioxidant with antiproliferative effect on cultured human cancer cell lines[J].Evidence-Based Complementary and Alternative Medicine,2013,2 013:1-8.
[8] LISA M D,KIMBERLY L C.Impacts of antibiotic use in agriculture:What are the benefits and risks?[J].Current Opinion in Microbiology,2014,19:37-44.
[9] HUANG Dan-min,LI Yun-hong,CUI Feng-jie,et al.Purification and characterization of a novel polysaccharide-peptide complex from Clinacanthus nutans Lindau leaves[J].Carbohydrate Polymers,2016,137:701-708.
[10] 岳鹍,潘志恒,孙勇民.混料设计优化干巴菌多糖复合酶法提取工艺[J].保鲜与加工,2018,18(5):91-96.
[11] WANG Yuan-yuan,WANG Xue-yu,XIONG Ying,et al.Extraction optimization,separation and antioxidant activity of Luffa Cylindrica polysaccharides[J].Food and Bioproducts Processing,2019,116:98-104.
[12] HU Wei-chao,ZHAO Yu-qing,YANG Yong,et al.Microwave-assisted extraction,physicochemical characterization and bioactivity of polysaccharides from Camptotheca acuminata fruits[J].International Journal of Biological Macromolecules,2019,133:127-136.
[13] 杨孝辉,郭君.响应面法优化超临CO2流体萃取淮山多糖工艺[J].食品工业科技,2019,40(7):185-189,198.
[14] 巫永华,刘恩岐,张建萍,等.超声微波协同酶法提取黄精多糖与抗氧化特性分析[J].食品工业,2019,40(5):171-176.
[15] 郭淼,宋江峰,王传凯.火龙果茎多糖的超高压提取及抗氧化活性研究[J].热带作物学报,2017,38(7):1 371-1 376.
[16] XU Guan-yi,LIAO Ai-mei,HUANG Ji-hong,et al.Evaluation of structural,functional,and anti-oxidant potential of differentially extracted polysaccharides from potatoes peels[J].International Journal of Biological Macromolecules,2019,129:778-785.
[17] 王丽霞,刘孟宗,王芳,等.铁皮石斛多糖提取及抗氧化活性研究[J].中国食品添加剂,2019,30(2):85-90.
[18] 张占军,王富花,葛洪,等.响应面法优化百合多糖超声辅助提取工艺[J].食品研究与开发,2017,38(18):40-44.
[19] SHU Xu,ZHANG Yan-fen,JIA Jin-xia,et al.Extraction,purification and properties of water-soluble polysaccharides from mushroom Lepista nuda[J].International Journal of Biological Macromolecules,2019,128:858-869.
[20] YUAN Ye,LIU Yang,LIU Meng-di,et al.Optimization extraction and bioactivities of polysaccharide from wild,Russula griseocarnosa[J].Saudi Pharmaceutical Journal,2017,25(4):523-530.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.