•  
  •  
 

Abstract

In order to improve the content of linear maltooligosaccharides with specific degree of polymerization in the enzymatic hydrolysis products, the effects were investigated including from the membrane separation, activated carbon adsorption separation, sephadex column chromatography and ion exchange resin column chromatography. The results showed that the membrane separation, activated carbon adsorption separation, sephadex column chromatography and Na+/Ca2+ ion exchange resins were all not conducive to continuous batch operation. In contrast, K+ ion exchange resins had better effects. Compared with the initial enzymatic hydrolysate, the proportion of G3~G6 and G5+G6 in the permeable solution after separation and purification by K+ ion exchange resin increased by 39.53% and 8.13%, and the recovery reached 78.11% and 63.89%, respectively. In addition, the purity and recovery of G3~G6 and G5+G6 in the end product were further improved by using the simulated moving bed with K+ ion exchange resin as fixed phase adsorbent. This study can provide a reference for the continuous separation and purification of straight malt oligosaccharides in industry.

Publication Date

7-28-2020

First Page

1

Last Page

5

DOI

10.13652/j.issn.1003-5788.2020.07.001

References

[1] 张百胜.麦芽低聚糖在食品生产中的应用[J].农产品加工:学刊,2007(7):91-92.
[2] BEN Ali M,MHIRI S,MEZGHANI M,et al.Purification and sequence analysis of the atypical maltohexaose-forming alpha-amylase of the B-stearothermophilus US100[J].Enzyme and Microbial Technology,2001,28(6):537-542.
[3] 毛跟年,杨亚洲,许牡丹,等.磷酸寡糖前体——麦芽低聚糖制备工艺的研究[J].陕西科技大学学报(自然科学版),2003,21(6):24-27.
[4] 朱明,吴嘉根.麦芽四糖的性质及在食品中的应用[J].冷饮与速冻食品工业,1999(4):23-24.
[5] KIMURA T,NAKAKUKI T.Maltotetraose,a new saccharide of tertiary property[J].Starch-Strke,2010,42(4):151-157.
[6] LEACH H W,HEBEDA R E,HOLIK D J.Enzymatically produced maltose-maltotriose starch hydrolysate:US,4113509[P].1978-09-12.
[7] 陈殿宁,李才明,顾正彪,等.玉米淀粉浓度对酶法制备直链麦芽低聚糖的影响[J].食品与发酵工业,2019,45(13):15-22.
[8] LIU Wen-jing,LI Zhao-feng,GU Zheng-biao,et al.Effects of microwave pretreatment on liquification of corn starch[J].Food and Fermentation Industries,2013,39(1):21-25.
[9] ROUSSEL X,LANCELON-PIN C,VIKSO-NIELSEN A,et al.Characterization of substrate and product specificity of the purified recombinant glycogen branching enzyme of Rhodothermus obamensis[J].Biochimica Et Biophysica Acta-General Subjects,2013,1 830(1):2 167-2 177.
[10] 张延滨,秦洪波,魏巍.用阿贝折射仪测定糖浓度[J].黑龙江医学,1996(1):31-32.
[11] AL-DEGS Y S,EL-BARGHOUTHI M I,EL-SHEIKH A H,et al.Effect of solution pH,ionic strength,and temperature on adsorption behavior of reactive dyes on activated carbon[J].Dyes and Pigments,2008,77(1):16-23.
[12] HONG S B,CHOI J H,CHANG Y K,et al.Production of high-purity fucose from the seaweed of Undaria pinnatifida through acid-hydrolysis and simulated-moving bed purification[J].Separation and Purification Technology,2019(213):133-141.
[13] 于伟,李良玉,李朝阳,等.顺序式模拟移动色谱分离木糖醇母液的前处理工艺研究[J].中国食品添加剂,2015(10):70-76.
[14] 陈献富,季华,范益群.纳滤膜在功能性低聚糖分离纯化中的应用研究进展[J].化工进展,2018,38(1):394-403.
[15] 徐贵华,刘钟栋,陈肇锬.小麦淀粉制备麦芽三糖的研究[J].河南工业大学学报(自然科学版),2002,23(3):1-4.
[16] 冯咏梅,常秀莲,王文华,等.葡聚糖凝胶过滤层析分离低聚半乳糖[J].北京化工大学学报(自然科学版),2009,36(1):73-76.
[17] 毛跟年,柯蕾,李彦军,等.离子交换法分离纯化麦芽低聚糖[J].食品工业科技,2005(4):127-128.
[18] MAUNO V,PAULI O,JUKKA N,et al.Site-directed mutagenesis of a thermostable α-amylase from bacillus stearothermophilus:Putative role of three conserved residues1[J].The Journal of Biochemistry,1990,107(2):267-272.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.