SsDNA-templated fluorescent copper nanoclusters for label free detection of tetracyclines

Authors

YANG Li-xia, Changsha Institute for Food and Drug Control, Changsha, Hunan 410036, China
ZENG Xi-ke, Changsha Institute for Food and Drug Control, Changsha, Hunan 410036, China
YI Zi, Changsha Institute for Food and Drug Control, Changsha, Hunan 410036, China

Abstract

In this paper, a novel and label-free fluorescence sensing strategy has been developed for tetracyclines (TCs) detection in food sample by using poly(thymine) (poly T)-templated copper nanoclusters (CuNCs) as fluorescent probes. Under optimized conditions, the label-free fluorescent sensor achieved highly sensitive and selective detection of TCs with a detection limit of 0.01 μmol/L, and with a detection limit of 48 μg/kg in real samples. Furthermore, the method was successfully applied to the detection of TCs in milk and chicken samples. The relative standard deviation was less than 4.01%, and the recovery rate was between 97.67% and 110.23%. The strategy was free of any fluorescence dye label or complex DNA sequence design, and all the processes were performed within 10 minutes at room temperature. Therefore, it could offer a simple, rapid, and label-free sensing platform for fluorescence detection of TCs in food.

Publication Date

2-28-2021

First Page

52

Last Page

57

DOI

10.13652/j.issn.1003-5788.2021.02.010

Recommended Citation

Li-xia, YANG; Xi-ke, ZENG; and Zi, YI (2021) "SsDNA-templated fluorescent copper nanoclusters for label free detection of tetracyclines," Food and Machinery: Vol. 37: Iss. 2, Article 10.
DOI: 10.13652/j.issn.1003-5788.2021.02.010
Available at: https://www.ifoodmm.cn/journal/vol37/iss2/10

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