Abstract
[Objective] To isolate and screen novel Weizmannia coagulans strains with high stress tolerance. [Methods] Using commercially fermented pickle brine as the sample, W. coagulans strains were isolated, purified, and identified by selective culture medium methods, morphological observation, physiological and biochemical characterization, and 16S rDNA sequencing. Taking survival rate as the evaluation index, the stress tolerance of the isolated strains and three laboratory-preserved W. coagulans strains was determined under high-temperature (80–100 ℃), acidic (pH 2.0–3.0), and bile salt (0.1%–0.5%) conditions. Principal component analysis (PCA) was applied to comprehensively assess the stress tolerance of the tested strains. [Results] Two W. coagulans strains, WC 412 and WC 413, were screened. The results of stress tolerance determination and comprehensive evaluation showed that strain WC 412 exhibited the best overall stress tolerance, followed by WC 413, S17, S15, and S8. [Conclusion] Two novel W. coagulans strains, WC 412 and WC 413, were obtained from commercially fermented pickle brine. Among them, strain WC 412 exhibited excellent comprehensive stress tolerance and has high potential for development and application.
Publication Date
7-13-2026
First Page
21
Last Page
28
DOI
10.13652/j.spjx.1003.5788.2025.80517
Recommended Citation
Xue, XIAO; Wendi, YU; Yuanliang, HU; Xicai, ZHANG; and Xiang, YU
(2026)
"Screening of Weizmannia coagulans with high stress tolerance,"
Food and Machinery: Vol. 42:
Iss.
6, Article 3.
DOI: 10.13652/j.spjx.1003.5788.2025.80517
Available at:
https://www.ifoodmm.cn/journal/vol42/iss6/3
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