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Corresponding Author(s)

张炜（1972—），女，青海师范大学教授，博士。E-mail：zhangwei@qhnu.edu.cn

Abstract

[Objective] Using Dunaliella spp. powder as the raw material, to explore the effect of pH value, alkali dissolution temperature, alkali dissolution time, and solid-liquid ratio on Dunaliella spp. protein extraction. [Methods] The optimal conditions are screened by single-factor experiments. Response surface experiments are designed by the Box-Behnken method to determine the optimal extraction process parameters for Dunaliella spp. protein with the Dunaliella spp. protein extraction rate as the indicator. Additionally, the group composition, secondary structure, amino acid composition, and functional properties of Dunaliella spp. protein are determined. [Results] The optimal extraction process conditions for Dunaliella spp. protein is pH 10.54, alkali dissolution temperature 51 ℃, alkali dissolution time 2.1 h, and a solid-liquid ratio of 1:206 (g/mL). Under these conditions, the extraction rate of Dunaliella spp. protein is 52.98%. At 50 ℃, Dunaliella spp. protein exhibits the highest water and oil holding capacity. At a mass fraction of 0.6%, Dunaliella spp. protein exhibits the highest foaming property (40%), foam stability (62.5%), emulsifying property (62%), and emulsifying stability (66%). Structural analysis shows that the secondary structure of Dunaliella spp. protein is mainly β-sheet (26.79%), with essential amino acids accounting for 32.94%, and hydrophobic amino acids accounting for 39.52%. [Conclusion] The optimized extraction process for Dunaliella spp. protein can significantly increase protein extraction rate.

Publication Date

7-25-2026

First Page

136

Last Page

144

DOI

10.13652/j.spjx.1003.5788.2025.80565

References

[1] 白仁奥.紫菜作为替代蛋白质可持续来源研究进展 [J].绿色科技,2024,26(18):257-264,276.Bai R A.Research progress of laver as a sustainable source of alternative protein [J].Journal of Green Science and Technology,2024,26(18):257-264,276.

[2] Karyotis T,Iliadis C,Noulas C,et al.Preliminary research on seed production and nutrient content for certain quinoa varieties in a saline –sodic soil [J].Journal of Agronomy and Crop Science,2003,189(6):402-408.

[3] 吉红,肖芬芬,邢君霞,等.微藻资源在水产饲料中应用的研究进展 [J].饲料工业,2020,41(16):1-12.Ji H,Xiao F F,Xing J X,et al.Research progress on the application of microalgae resources in aquatic feed [J].Feed Industry,2020,41(16):1-12.

[4] 陆超华,谢文造.利用盐藻开发功能食品的前景 [J].广州食品工业科技,1995,11(4):14-17.Lu C H,Xie W Z.The prospects of developing functional foods using Dunaliella salina [J].Guangzhou Food Science and Technology,1995,11(4):14-17.

[5] 杨雪梅,吴起元.盐藻的研究与开发 [J].生命科学,1994,6(5):7-10.Yang X M,Wu Q Y.Research and development of Dunaliella Salina [J].Chinese Bulletin of Life Sciences,1994,6(5):7-10.

[6] 罗成伟,范春霞,叶帅静,等.微藻的活性成分及应用研究进展[J].农业灾害研究,2024,14(12):10-12.Luo C W,Fan C X,Ye S J,et al.Research progress on the active components and applications of microalgae [J].Journal of Agricultural Catastrophology,2024,14(12):10-12.

[7] 孔凡晶,郑绵平,张洪霞,等.盐湖农业及其发展战略研究 [J].中国工程科学,2019,21(1):148-152.Kong F J,Zheng M P,Zhang H X,et al.Salt lake agriculture and its development strategy [J].Strategic Study of CAE,2019,21(1):148-152.

[8] 周毅航,陈晓倩,杨敏,等.杜氏盐藻的基础生物学和开发应用研究进展 [J].广西植物,2025,45(4):793-808.Zhou Y H,Chen X Q,Yang M,et al.Research advances on basic biology and development and application of Dunaliella [J].Guihaia,2025,45(4):793-808.

[9] 李正华,江树勋,蔡桂琴,等.盐藻分子生物学研究概况 [J].海洋科学,2005,29(6):69-72.Li Z H,Jiang S X,Cai G Q,et al.Review on molecular biology of Dunaliella sp.[J].Marine Sciences,2005,29(6):69-72.

[10] 古玉环,陈军.盐生杜氏藻 Dunaliella salina 的生物学特性与培养研究 [J].西北师范大学学报 (自然科学版 ),1995,31(4):52-55.Gu Y H,Chen J.Biological properties and culture conditions of Dunaliella salina [J].Journal of Northwest Normal University (Natural Science ),1995,31(4):52-55.

[11] 高风正,葛保胜,向文洲,等.生物技术研究引领中国微藻产业发展的六十年:回顾与展望 [J].中国科学:生命科学,2021,51(1):26-39.Gao F Z,Ge B S,Xiang W Z,et al.Development of microalgal industries in the past 60 years due to biotechnological research in China:a review [J].Scientia Sinica (Vitae ),2021,51(1):26-39.

[12] 景素琴,丁红霞.运城盐湖盐藻产业的前景展望 [J].盐业与化工,2016,45(11):8-10.Jing S Q,Ding H X.Prospect on Dunaliella salina industry of Yuncheng salt lake [J].Journal of Salt and Chemical Industry,2016,45(11):8-10.

[13] 郭金耀,杨晓玲.盐藻蛋白质的提取分离 [J].食品科技,2010,35(8):232-235.Guo J Y,Yang X L.Extraction and separation of Dunaliella salina protein [J].Food Science and Technology,2010,35(8):232-235.

[14] 萨如拉,贾丽丽,杨昕雨,等.碱溶酸沉法优化大豆粕分离蛋白的提取工艺 [J].粮食与饲料工业,2025 (3):97-103.Sa R L,Jia L L,Yang X Y,et al.Extraction process of soybean meal protein isolate optimized by alkaline solution and acid isolation [J].Cereal & Feed Industry,2025 (3):97-103.

[15] 祝连彩,唐士金,周丽.考马斯亮蓝 G 250法测定蛋白质含量的教学实践及方法学探讨 [J].教育教学论坛,2020 (23):266-269.Zhu C L,Tang S J,Zhou L.Teaching practice and methodological investigation of protein content determination using coomassie brilliant blue G 250[J].Education Teaching Forum,2020 (23):266-269.

[16] 邵端阳,常鑫,李东辉.响应面法优化碱蓬蛋白质提取工艺及抗氧化活性分析 [J].锦州医科大学学报,2024,45(6):1-7.Shao D Y,Chang X,Li D H.Analysis on optimization of extraction technology of Suaeda salsa protein by response surface methodology and its antioxidant activity [J].Journal of Jinzhou Medical University,2024,45(6):1-7.

[17] 杨慧芬,李思燕,段兴芳,等.超声辅助碱溶酸沉法提取黑牛肝菌蛋白质工艺优化 [J].广州化工,2025,53(2):32-35,39.Yang H F,Li S Y,Duan X F,et al.Optimization of ultrasonic-assisted alkali-solution and acid-isolation method for extraction of protein from boletus aereus [J].Guangzhou Chemical Industry,2025,53(2):32-35,39.

[18] 赵巧丽,高永琴,谭杰丹,等.碱溶酸沉法提取螺旋藻分离蛋白 工 艺 优 化 及 性 质 分 析 [J].食 品 与 机 械,2025,41(6):182-189.Zhao Q L,Gao Y Q,Tan J D,et al.Optimization of extraction process and property analysis of spirulina protein isolate by alkaline dissolution and acid precipitation [J].Food & Machinery,2025,41(6):182-189.

[19] 薛建娥,王英翰,洪金明,等.响应面优化核桃蛋白的提取及性质研究 [J].食品工业,2024,45(5):49-54.Xue J E,Wang Y H,Hong J M,et al.Response surface optimization for the extraction and properties of walnut protein[J].The Food Industry,2024,45(5):49-54.

[20] 高晓杰,聂玲萍,罗紫嫣,等.双孢蘑菇糖蛋白提取工艺优化[J].中南农业科技,2025,46(2):30-33.Gao X J,Nie L P,Luo Z Y,et al.Optimization of the extraction process of glycoproteins from Agaricus bisporus [J].South-Central Agricultural Science and Technology,2025,46(2):30-33.

[21] 马琰彬,张炜,隋成博,等.泡沫分离刚毛藻蛋白工艺优化及其功能特性分析 [J].食品研究与开发,2025,46(2):119-128.Ma Y B,Zhang W,Sui C B,et al.Optimization of foam separation process of Cladophora protein and analysis of functional characteristics [J].Food Research and Development 2025,46(2):119-128.

[22] 马琰彬,张炜,赵鹤然.青海湖刚毛藻蛋白泡沫分离工艺优化及理化性质 [J].精细化工,2025,42(12):2 717-2 726.Ma Y B,Zhang W,Zhao H R.Optimization of protein foam separation process and physicochemical properties of Cladophora Qinghaiensis [J].Fine Chemicals,2025,42(12):2 717-2 726.

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