Abstract
[Objective］The variations in inorganic element content and the safety of heavy metals and harmful elements in Ranunculi ternati radix from different origins were investigated.［Methods］The quantitative analysis of 24 elements in the samples was performed by inductively coupled plasma mass spectrometry (ICP-MS). Health risk assessment was conducted using estimated daily intake (EDI), target hazard quotient (THQ), and carcinogenic risk (CR). Principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used for origin identification analysis and differential element screening. [Results] All 25 Ranunculi ternati radix samples from different origins contain abundant macronutrients (K, Na, Ca, and Mg) and micronutrients (Fe, Cu, Zn, Cr, Mn, Ni, V, Co, Mo, and Se) essential for the human body. The contents of heavy metals and harmful elements Pb, Cd, As, Hg, and Cu are 0.630.51, 0.050.11, 0.260.62, 0.010.10, and 32.96~167.25 mg/kg, respectively. Among them, the Cu content exceeds the limit for botanical drugs in the Chinese Pharmacopoeia (2020 edition). In terms of health risk assessment, the EDI analysis reveals that the estimated maximum daily intakes of Cr, Al, Ni, Pb, As, and Cu for adults and children from Ranunculi ternati radix in all or some origins exceed 10% of the provisional tolerable daily intake (PTDI), with some exceeding the PTDI value, indicating health risks. In the THQ analysis, the target hazard quotient values of Cr, Al, Cu, and As for adults and children from Ranunculi ternati radix in all or some origins exceed the standard THQ, indicating health hazard risks. In the CR analysis, the potential carcinogenic risk indices of Cd and As, as well as the total potential carcinogenic risk index of the three heavy metals Cd, As, and Pb, range from 1×10−6 to 1×10−4, indicating potential carcinogenic risks. The PCA identifies elements such as V, Al, Cs, Co, As, Fe, Mn, Tl, Cr, Pb, K, Ni, Sr, Cu, Ag, Cd, Hg, Zn, Ca, Na, Mo, Mg, and Se as the characteristic elements of Ranunculi ternati radix. The PLS-DA identifies four elements, including K, Mg, Fe, and Al, as effective traceability indicators for the origin of Ranunculi ternati radix.［Conclusion］The established method can rapidly and accurately analyze the inorganic element contents in Ranunculi ternati radix. When Ranunculi ternati radix is ingested for a long term, attention should be paid to controlling the dosage and duration of administration to reduce the health risks caused by elements such as Cr, Al, As, Cu, Ni, Pb, and Cd.
Publication Date
7-25-2026
First Page
28
Last Page
41
DOI
10.13652/j.spjx.1003.5788.2025.80582
Recommended Citation
Juan, Li; Xiaotong, Geng; Weijie, Yao; Tao, Zhou; and Jingjing, Bi
(2026)
"Analysis of inorganic element content and health risk assessment of Ranunculi ternati radix from different origins,"
Food and Machinery: Vol. 42:
Iss.
7, Article 4.
DOI: 10.13652/j.spjx.1003.5788.2025.80582
Available at:
https://www.ifoodmm.cn/journal/vol42/iss7/4
References
[1] 国家药典委员会.中华人民共和国药典:一部 [S].2020版.北京:中国医药科技出版社,2020:333.State Pharmacopoeia Committee.Chinese pharmacopoeia:part I[S].2020 ed.Beijing:China Pharmaceutical Science and Technology Press,2020:333.
[2] 河南信阳专署中医诊所.猫爪草治疗淋巴结结核 [J].中国防痨,1958 (5):24-25.Tcm Clinic of Xinyang Commissioner's Office,Henan Province.Ranunculi ternati radix in the treatment of tuberculous lymphadenitis [J].Chinese Journal of Antituberculosis,1958 (5):24-25.
[3] 刘娜,李兰涛.基于 CiteSpace 的知识图谱分析猫爪草研究现状及发展趋势 [J].今日药学,2025,35(3):196-204.Liu N,Li L T.Current status and development trends of Ranunculi ternati radix research:a knowledge map analysis based on CiteSpace [J].Pharmacy Today,2025,35(3):196-204.
[4] 孙岩,卢智玲,邹耀华,等.基于 ICP-MS 结合多元统计的林下山参中矿物质元素分析 [J].中药材,2024，47(7):1 738-1 744.Sun Y,Lu Z L,Zou Y H,et al.Mineral element analysis of wild Ginseng using ICP-MS combined with multivariate statistics [J].Journal of Chinese Medicinal Materials,2024,47(7):1 738-1 744.
[5] 何秋云,何敏,陈明,等.基于 ICP-MS 结合化学计量学分析五指毛桃中微量元素含量及健康风险评估 [J].中药材,2024,47(10):2 549-2 554.He Q Y,He M,Chen M,et al.Trace element content and health risk assessment of Morinda officinalis based on ICP-MS combined with chemometric analysis [J].Journal of Chinese Medicinal Materials,2024,47(10):2 549-2 554.
[6] 刘浩,郭海涛,姬爱熙,等.ICP-MS 法测定松花粉无机元素含量及安全性、健康风险评估 [J].河北科技大学学报,2025,46(1):67-76.Liu H,Guo H T,Ji A X,et al.Determination of inorganic elements in pine pollen by ICP-MS and safety and health risk assessment [J].Journal of Hebei University of Science and Technology,2025,46(1):67-76.
[7] 左甜甜,张磊,石上梅,等.10种根和根茎类中药材中重金属及有害元素的风险评估及最大限量理论值 [J].药物分析杂志,2020,40(10):1 870-1 876.Zuo T T,Zhang L,Shi S H,et al.Risk assessment and theoretical limits for heavy metals and harmful elements in 10 types of root and rhizome Chinese medicinal materials [J].Chinese Journal of Pharmaceutical Analysis,2020,40(10):1 870-1 876.
[8] 冯俊,李清清.微波消解—电感耦合等离子体质谱法测定根茎类中药材中多种重金属元素含量 [J].食品与药品,2022,24(5):432-437.Feng J,Li Q Q.Determination of heavy metal elements in rhizomatic traditional Chinese medicine by microwave digestion-inductively coupled plasma mass spectrometry [J].Food and Drug,2022,24(5):432-437.
[9] 全山丛,汪学昭,郑汉臣,等.中药猫爪草的微量元素分析 [J].微量元素与健康研究,1997 (2):29-30.Quan S C,Wang X Z,Zheng H C,et al.Trace element analysis of Ranunculi ternati radix [J].Studies of Trace Elements and Health,1997 (2):29-30.
[10] 陈军,姚成,欧阳平凯.ICP-AES 法测定猫爪草中常量及微量元素 [J].光谱学与光谱分析,2005 (4):560-562.Chen J,Yao C,Ouyang P K.Determination of constant and trace elements in Ranunculus ternatus thunb by ICP-AES [J].Spectroscopy and Spectral Analysis,2005 (4):560-562.
[11] 任盼莹,程梦娟,王丰青,等.ICP-MS 结合化学模式识别分析野生地黄产区土壤中矿物元素分布特征 [J].天然产物研究与开发,2025,37(5):859-868,827.Ren P Y,Cheng M J,Wang F Q,et al.Analysis of distribution characteristics of mineral elements in the soil of wild Rehmannia glutinosa production area by ICP-MS combined with chemical pattern recognition [J].Natural Product Research and Development,2025,37(5):859-868,827.
[12] 胡利喆,李鹏,朱宏,等.黄芪中重金属含量分析及风险评估[J].食品安全质量检测学报,2025,16(9):120-128.Hu L Z,Li P,Zhu H,et al.Analysis and risk assessment of |heavy metal content in Astragalus membranaceus [J].Journal of Food Safety & Quality,2025,16(9):120-128.
[13] 张思维,李东梅,刘小云,等.甘肃产黄芪重金属含量特征分析及健康风险评估 [J].食品与机械,2023，39(5):38-42,69.Zhang S W,Li D M,Liu X Y,et al.Analysis of heavy metal content and health risk assessment of Astragalus membranaceus in Gansu province [J].Food & Machinery,2023,39(5):38-42,69.
[14] 李运,戚鹏飞,张晓萍,等.基于 ICP-MS 技术的六神曲中无机元素分析及其风险评估 [J].中国无机分析化学,2024,14(9):1 340-1 352.Li Y,Qi P F,Zhang X P,et al.Analysis and risk assessment of inorganic elements in Liushenqu based on ICP-MS technology[J].Chinese Jorunal of Inorganic Analytical Chemistry,2024,14(9):1 340-1 352.
[15] 陈雄,梁军,李青,等.ICP-MS 法测定安化黑茶中 4种重金属元素含量及健康风险评估 [J].食品安全导刊,2024 (24):68-70.Chen X,Liang J,Li Q,et al.Determination of four heavy metals in Anhua black tea by ICP-MS and health risk assessment [J].China Food Safety Magazine,2024 (24):68-70.
[16] 闫研,秦斌,梁雪,等.17种中药材中重金属污染分析与健康风险评估 [J].药学研究,2023,42(11):902-908.Yan Y,Qin B,Liang X,et al.Pollution analysis and health risk assessment of heavy metals in seventeen kinds of Chinese medicinal materials [J].Journal of Pharmaceutical Research,2023,42(11):902-908.
[17] 王巧,于永杰,王紫怡,等.基于 ICP-MS 的不同产地丹参元素差异分析及健康风险评估 [J].中药材,2023,46(9):2 224-2 232.Wang Q,Yu Y J,Wang Z Y,et al.Element difference analysis and health risk assessment of Salviae miltiorrhizae radix et rhizoma from different producing areas based on ICP-MS [J].Journal of Chinese Medicinal Materials,2023,46(9):2 224-2 232.
[18] 国家药典委员会.中华人民共和国药典:四部 [S].2020版.北京:中国医药科技出版社,2020:520.State Pharmacopoeia Committee.Chinese pharmacopoeia:part IV[S].2020 ed.Beijing:China Pharmaceutical Science and Technology Press,2020:520.
[19] Limón G,Samhadaneh N M,Pironti A,et al.Aldehyde accumulation in Mycobacterium tuberculosis with defective proteasomal degradation results in copper sensitivity [J].mBio,2023,14(4):e0036323.
[20] 徐志弘.Rv1273 c-Rv 1272 c与铜转运及 rpoC突变对利福平耐药结核分枝杆菌转录谱的调控研究 [D].上海:上海交通大学,2018:5-6.Xu Z H.Rv1273 c-Rv 1272 c for copper transport and rpoC mutations in rifampicin-resistant Mycobacterium tuberculosis[D].Shanghai:Shanghai Jiao Tong University,2018:5-6.
[21] Wolschendorf F,Ackart D,Shrestha T B,et al.Copper resistance is essential for virulence of Mycobacterium tuberculosis [J].Proceedings of the National Academy of Sciences of the United States of America,2011,108(4):1 621-1 626.
[22] Zimnicka A M,Tang H Y,Guo Q,et al.Upregulated copper transporters in hypoxia-induced pulmonary hypertension [J].PLoS One,2014,9(3):e90544.
[23] White C,Kambe T,Fulcher Y G,et al.Copper transport into the secretory pathway is regulated by oxygen in macrophages[J].Journal of Cell Science,2009,122(9):6.
[24] 刘骏达,钟薇薇,鲁显福,等.铜死亡与铜代谢相关疾病研究进展 [J].江苏大学学报 (医学版 ),2022,32(4):318-325.Liu J D,Zhong W W,Lu X F,et al.Research advances on copper death and copper metabolism-related diseases [J].Journal of Jiangsu University (Medicine Edition ),2022,32(4):318-325.
[25] United States Environmental Protection Agency (USEPA ).Risk based concentration table- 0249 [R].Washington DC:USEPA,2000:2-3.
[26] 李晓玲,童巧珍,石雨荷,等.龙牙百合不同器官重金属含量分布特征及健康风险评估 [J].食品与机械,2025,41(7):56-64.Li X L,Tong Q Z,Shi Y H.Characteristics of heavy metal content distribution in different organs of Lilium brownii and its health risk assessment [J].Food & Machinery,2025,41(7):56-64.
[27] 考庆君,吴坤.铬的生物学作用及毒性研究进展 [J].中国公共卫生,2004 (11):122-124.Kao Q J,Wu K.Research progress on the biological effects and toxicity of chromium [J].Chinese Journal of Public Health,2004 (11):122-124.
[28] 余晓星,彭宝成.三氯化铬的蓄积毒性及其对骨髓细胞微核率和精子形成的影响 [J].河北医科大学学报,1997 (3):14-16.Yu X X,Peng N C.Accumulative toxicity and effects on marrow cell micronucles rate and sperm formation of CrCl3[J].Journal of Hebei Medical University,1997 (3):14-16.
