Establishment of an indirect competitive ELISA for sulfadimethoxine

Authors

• Han Lu, College of Food and Bioengineering , Henan University of Science and Technology , Luoyang , Henan 471000 , China;Henan International Joint Laboratory of Food Green Processing and Quality Safety Control , Luoyang , Henan 471000 , China
• Huang Mingyue, College of Food and Bioengineering , Henan University of Science and Technology , Luoyang , Henan 471000 , China;Henan International Joint Laboratory of Food Green Processing and Quality Safety Control , Luoyang , Henan 471000 , China
• Yu Xiang, College of Food and Bioengineering , Henan University of Science and Technology , Luoyang , Henan 471000 , China;Henan International Joint Laboratory of Food Green Processing and Quality Safety Control , Luoyang , Henan 471000 , China
• Li Sijia, College of Food and Bioengineering , Henan University of Science and Technology , Luoyang , Henan 471000 , China;Henan International Joint Laboratory of Food Green Processing and Quality Safety Control , Luoyang , Henan 471000 , China
• Cao Jinbo, School of Chemistry , South China Normal University , Guangzhou , Guangdong 510000 , China
• Wang Yao, College of Food and Bioengineering , Henan University of Science and Technology , Luoyang , Henan 471000 , China;Henan International Joint Laboratory of Food Green Processing and Quality Safety Control , Luoyang , Henan 471000 , ChinaFollow
• Kang Huaibin, College of Food and Bioengineering , Henan University of Science and Technology , Luoyang , Henan 471000 , China;Henan International Joint Laboratory of Food Green Processing and Quality Safety Control , Luoyang , Henan 471000 , China

Corresponding Author(s)

王耀（1986—），男，河南科技大学教授，博士。E-mail：wangyao@haust.edu.cn

Abstract

[Objective] To establish an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for the detection of sulfadimethoxine (SDM) in animal-derived foods. [Methods] An artificial antigen is synthesized by conjugating SDM with carrier proteins by the diazotization method. A highly sensitive antibody against SDM is prepared by immunizing animals. Based on this, an ic-ELISA is established and the detection system is optimized. Its detection performance is characterized, and spiked recovery tests are carried out. [Results] When the concentration of the coating antigen is 0.500 μg/mL, the dilution ratio of the horseradish peroxidase-labeled goat anti-rabbit secondary antibody is 1:5000, the reaction time of the primary antibody is 15 min, the reaction time of the secondary antibody is 30 min, and the chromogenic reaction time is 10 min, the established ic-ELISA exhibits an IC50 of 9.33 ng/mL, a detection range of 0.76~114.82 ng/mL, and a limit of detection of 0.74 ng/mL, with good precision and specificity, and satisfactory spiked recovery rates in milk, chicken, and pork samples. [Conclusion] This method can be applied to the sensitive, precise, specific and accurate detection of SDM residues in real samples of milk, chicken, and pork, providing an effective means for efficient on-site screening.

Publication Date

7-25-2026

First Page

52

Last Page

59

DOI

10.13652/j.spjx.1003.5788.2025.80598

Recommended Citation

Lu, Han; Mingyue, Huang; Xiang, Yu; Sijia, Li; Jinbo, Cao; Yao, Wang; and Huaibin, Kang (2026) "Establishment of an indirect competitive ELISA for sulfadimethoxine," Food and Machinery: Vol. 42: Iss. 7, Article 6.
DOI: 10.13652/j.spjx.1003.5788.2025.80598
Available at: https://www.ifoodmm.cn/journal/vol42/iss7/6

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