Determination of five fluorescent whitening agents in prepared dishes by gel permeation chromatography-high performance liquid chromatography

Authors

• Li Bingbing, Center for Disease Control and Prevention of Yancheng , Yancheng , Jiangsu 224000 , China;Yancheng Center of Jiangsu Food Safety Risk Monitoring , Yancheng , Jiangsu 224000 , China
• Wang Chunlei, Center for Disease Control and Prevention of Yancheng , Yancheng , Jiangsu 224000 , China;Yancheng Center of Jiangsu Food Safety Risk Monitoring , Yancheng , Jiangsu 224000 , China
• Huang Zhenzhen, Center for Disease Control and Prevention of Yancheng , Yancheng , Jiangsu 224000 , China;Yancheng Center of Jiangsu Food Safety Risk Monitoring , Yancheng , Jiangsu 224000 , China
• Dai Li, Center for Disease Control and Prevention of Yancheng , Yancheng , Jiangsu 224000 , China;Yancheng Center of Jiangsu Food Safety Risk Monitoring , Yancheng , Jiangsu 224000 , China
• Wang Kun, Center for Disease Control and Prevention of Yancheng , Yancheng , Jiangsu 224000 , China;Yancheng Center of Jiangsu Food Safety Risk Monitoring , Yancheng , Jiangsu 224000 , ChinaFollow

Corresponding Author(s)

王坤（1976—），男，盐城市疾病预防控制中心主任技师。E-mail：1059452181@qq.com

Abstract

[Objective] To establish a method for the determination of five fluorescent whitening agents (FWAs) in prepared dishes using gel permeation chromatography combined with high-performance liquid chromatography (GPC-HPLC). [Methods] The prepared dish samples were homogenized, ultrasonically extracted with acetonitrile, purified and reconstituted by GPC, then filtered before being analyzed by HPLC. The separation was performed on a Waters Symnetry Shield PR-C18 column (4.6 mm ×250 mm, 5.0 µm) using acetonitrile and water as the mobile phase with gradient elution. [Results] The five FWAs in the prepared dishes showed good linearity within the range of 2~500 µg/L, with correlation coefficients greater than 0.999. The limits of detection (LODs) ranged from 0.016 to 0.233 µg/kg, and the limits of quantification (LOQs) ranged from 0.05 to 0.78 µg/kg. At three spiked levels (low, medium, and high), the recoveries for the five FWAs were between 75.3% and 97.1%, with relative standard deviations (RSDs) ranging from 0.3% to 5.0%. [Conclusion] The developed method is accurate, reliable, and highly sensitive, making it suitable for the determination of FWAs in prepared dishes.

Publication Date

7-25-2026

First Page

77

Last Page

82

DOI

10.13652/j.spjx.1003.5788.2025.80528

Recommended Citation

Bingbing, Li; Chunlei, Wang; Zhenzhen, Huang; Li, Dai; and Kun, Wang (2026) "Determination of five fluorescent whitening agents in prepared dishes by gel permeation chromatography-high performance liquid chromatography," Food and Machinery: Vol. 42: Iss. 7, Article 9.
DOI: 10.13652/j.spjx.1003.5788.2025.80528
Available at: https://www.ifoodmm.cn/journal/vol42/iss7/9

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