Abstract
[Objective] To compare the effects of four pretreatment methods—hot pressing (HP), steam explosion (SE), ultrasound (US), and solid-state fermentation (SF)—on the structural characteristics and antioxidant activities of Polygonatum sibiricum polysaccharides (PSP), and to explore the potential application of PSP in fruit preservation. [Methods] Ten PSP samples are prepared. The yield, relative molecular mass, functional groups, thermal stability, surface morphology, and DPPH·, ABTS·, and OH· free radical scavenging abilities of the samples are determined. The PSP sample with low relative molecular mass obtained by SE treatment is loaded with Zn2+ to prepare a sprayable hydrogel, which is then applied in the preservation of “Shatangju” mandarins. [Results] The yields of PSP from SE and US treatments are the highest (~10%), and are higher than those from HP and SF treatments (P<0.05). The PSP samples from SE and US treatments have low relative molecular mass (32.92×103 to 49.15×103), which is significantly lower than that of samples from HP and SF treatments. All the ten samples show similar functional groups and thermal stability, while they differ in surface morphology. In the concentration range of 0.5 to 4.0 mg/mL, all the samples exhibit concentration-dependent scavenging rates against DPPH·, ABTS·, and OH· free radicals. The antioxidant activity of PSP from SE treatment is higher than that from the other treatments (P<0.05). The Zn2+-loaded sprayable hydrogel prepared from SE-derived PSP with low relative molecular mass effectively reduces the weight loss rate of “Shatangju” mandarins during storage, slows down the decline in fruit firmness and the progression of mildew, and exhibits good antifungal effects, with Zn2+ being the key component responsible for the antifungal activity. [Conclusion] SE treatment offers significant advantages in obtaining PSP with low relative molecular mass, improving the yield and antioxidant activity, and showing application potential in fruit preservation.
Publication Date
9-20-2026
First Page
96
Last Page
105
DOI
10.13652/j.spjx.1003.5788.2026.80398
Recommended Citation
Chengzhi, Hu; Yanli, Ma; Qingshan, Shen; Mengna, Li; Mi, Li; and Peng, Fei
(2026)
"Preparation process , antioxidant activity , and fruit preservation application of Polygonatum sibiricum polysaccharides with low relative molecular mass,"
Food and Machinery: Vol. 42:
Iss.
8, Article 13.
DOI: 10.13652/j.spjx.1003.5788.2026.80398
Available at:
https://www.ifoodmm.cn/journal/vol42/iss8/13
References
[1] 汪前,翁丽萍,刘军波,等.黄精多糖的制备、结构及构效关系研究进展 [J].食品科技,2025,50(6):214-221.Wang Q,Weng L P,Liu J B,et al.Research progress on preparation,structure and structure-activity relationship of Polygonatum polysaccharides [J].Food Science and Technology,2025,50(6):214-221.
[2] Zhao P,Li X,Wang Y,et al.Characterisation and saccharide mapping of polysaccharides from four common Polygonatum spp[J].Carbohydrate Polymers,2020,233:115836.
[3] Chen Z R,Zhu B J,Chen Z X,et al.Effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping analysis and pharmacological activity assays [J].Chinese Medicine,2022,17(1):97.
[4] Lee Q,Xue Z X,Luo Y J,et al.Low molecular weight polysaccharide of Tremella fuciformis exhibits stronger antioxidant and immunomodulatory activities than high molecular weight polysaccharide [J].International Journal of Biological Macromolecules,2024,281:136097.
[5] Jing Y S,Yan M,Zhang H,et al.Effects of extraction methods on the physicochemical properties and biological activities of polysaccharides from Polygonatum sibiricum [J].Foods,2023,12(10):2 088.
[6] 谢冰宗,李密,董彩文,等.黄精皂苷与多糖超声辅助提取工艺优化及降血糖活性研究 [J].食品与机械,2024,40(1):158-166,196.Xie B Z,Li M,Dong C W,et al.Study on the technology of ultrasonic assisted combined extraction of saponins and polysaccharides from Polygonatum sibiricum and its hypoglycemic activity [J].Food & Machinery,2024,40(1):158-166,196.
[7] 王晶,匡楚玉,夏智慧,等.蒸汽爆破燕麦体外消化特性及其对高脂诱导小鼠肥胖的影响 [J].食品与机械,2025,41(5):121-126.Wang J,Kuang C Y,Xia Z H,et al.In vitro digestion characteristics of steam-exploded oats and their effects on obesity induced by high-fat diet in mice [J].Food & Machinery,2025,41(5):121-126.
[8] Shen Q S,Zhang C H,Jia W,et al.Co-production of chondroitin sulfate and peptide from liquefied chicken sternal cartilage by hot-pressure [J].Carbohydrate Polymers,2019,222:115015.
[9] 杨壮,刘怡琳,李隆熙,等.固态发酵制备黄精多糖的工艺优化、理化特性及抗氧化活性 [J].食品与发酵工业,2024,50(7):92-98.Yang Z,Liu Y L,Li L X,et al.Process optimization,physicochemical properties,and antioxidant activity of Polygonatum sibiricum polysaccharide prepared by solid-state fermentation [J].Food and Fermentation Industries,2024,50(7):92-98.
[10] 曲梦锐,刘雨辰,王东营.壳聚糖—黄精精油复合涂膜对苹果果实的保鲜功效 [J].食品与机械,2025,41(2):115-120.Qu M R,Liu Y C,Wang D Y.Preservation performance of chitosan-Polygonatum essential oil composite coating film on apple fruits [J].Food & Machinery,2025,41(2):115-120.
[11] Shen Q S,Wang H,Zhang C H,et al.Liquefaction of porcine hoof shell to prepare peptone substitute by instant catapult steam explosion [J].Journal of Bioscience and Bioengineering,2020,129(4):467-475.
[12] Shen Q S,Zhang C H,Jia W,et al.Liquefaction of chicken sternal cartilage by steam explosion to isolate chondroitin sulfate [J].Carbohydrate Polymers,2019,215:73-81.
[13] Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding [J].Analytical Biochemistry,1976,72(1/2):248-254.
[14] Miller G L.Use of dinitrosalicylic acid reagent for determination of reducing sugar [J].Analytical Chemistry,1959,31(3):426-428.
[15] Dubois M,Gilles K A,Hamilton J K,et al.Colorimetric method for determination of sugars and related substances [J].Analytical Chemistry,1956,28(3):350-356.
[16] Shen Q S,Qi X D,Liu Y L,et al.Anti-obesity and gut microbiota modulation effects of chondroitin sulfate on obese mice induced by high-fat diet [J].International Journal of Biological Macromolecules,2025,298:139968.
[17] Wang S Q,Li G,Zhang X F,et al.Structural characterization and antioxidant activity of Polygonatum sibiricum polysaccharides [J].Carbohydrate Polymers,2022,291:119524.
[18] Su L L,Li X M,Guo Z J,et al.Effects of different steaming times on the composition,structure and immune activity of Polygonatum polysaccharide [J].Journal of Ethnopharmacology,2023,310:116351.
[19] Shen Q S,Ma Y L,Qin X J,et al.Steam explosion as a green method to treat animal waste:a mini-review [J].Process Safety and Environmental Protection,2024,181:43-52.
[20] Lee Q,Han X J,Zheng M F,et al.Preparation of low molecular weight polysaccharides from Tremella fuciformis by ultrasonic-assisted H2O2-VC method:structural characteristics,in vivo antioxidant activity and stress resistance [J].Ultrasonics Sonochemistry,2023,99:106555.
[21] Pan Y,Liu C J,Jiang S,et al.Ultrasonic-assisted extraction of a low molecular weight polysaccharide from Nostoc commune Vaucher and its structural characterization and immunomodulatory activity [J].Ultrasonics Sonochemistry,2024,108:106961.
[22] Cai M,Gao W J,Mao Y C,et al.The polysaccharides from mixed-culture fermentation of Huangjing:extraction,structural analysis and anti-lipid activity [J].LWT-Food Science and Technology,2025,233:118537.
[23] Qi Z H,Gao T X,Li J J,et al.Structural characterization and prebiotic potential of polysaccharides from Polygonatum sibiricum [J].Food Science and Human Wellness,2024,13(4):2 208-2 220.
[24] Yao H Y Y,Wang J Q,Yin J Y,et al.A review of NMR analysis in polysaccharide structure and conformation:progress,challenge and perspective [J].Food Research International,2021,143:110290.
[25] Cheng H N,Neiss T G.Solution NMR spectroscopy of food polysaccharides [J].Polymer Reviews,2012,52(2):81-114.
[26] Cheng J,He S Y,Wan Q,et al.Multiple fingerprinting analyses in quality control of Cassiae Semen polysaccharides[J].Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences,2018,1 077:22-27.
[27] Arab K,Ghanbarzadeh B,Ayaseh A,et al.Extraction,purification,physicochemical properties and antioxidant activity of a new polysaccharide from Ocimum album L.seed[J].International Journal of Biological Macromolecules,2021,180:643-653.
[28] Liu Y Y,Kan Y J,Huang Y T,et al.Physicochemical characteristics and antidiabetic properties of the polysaccharides from Pseudostellaria heterophylla [J].Molecules,2022,27(12):3 719.
[29] Chen N,Jiang T Y,Xu J X,et al.The relationship between polysaccharide structure and its antioxidant activity needs to be systematically elucidated [J].International Journal of Biological Macromolecules,2024,270:132391.
[30] 佟彤.柠檬草提取物纳米银的绿色合成及其抗菌保鲜作用研究 [D].长春:吉林大学,2022.Tong T.Green synthesis of silver nanoparticle using from lemon grass extract and its antibacterial and fresh-keeping effect [D].Changchun:Jilin University,2022.
[31] 于泽东.纳米银/壳聚糖复合涂膜中银的迁移及其对鲜食柑橘贮藏品质的影响 [D].重庆:西南大学,2022.Yu Z D.Migration of silver in nano silver/chitosan composite coating and its effect on the storage quality of fresh citrus [D].Chongqing:Southwest University,2022.
[32] Duan B,Gao Z J,Reymick O O,et al.Cinnamaldehyde promotes the defense response in postharvest citrus fruit inoculated with Penicillium digitatum and Geotrichum citri-aurantii [J].Pesticide Biochemistry and Physiology,2021,179:104976.
[33] Wang T L,Lu Z C,Wang X Q,et al.A compound of ZnO/PDMS with photocatalytic,self-cleaning and antibacterial properties prepared via two-step method [J].Applied Surface Science,2021,550:149286.
