Abstract
[Objective] To explore the preparation conditions, structural information, and in vitro hypoglycemic activities of acetylated Elaeagnus moorcroftii Wall. polysaccharides of Kashgar (A-EMP-K). [Methods] EMP-K is extracted by the water extraction and alcohol precipitation method, and modified by the acetic anhydride method. Then, A-EMP-K preparation conditions are optimized using Box–Behnken response surface methodology. Furthermore, structural characterization and in vitro hypoglycemic activity assays of A-EMP-K are performed. [Results] The optimal preparation conditions are a reaction time of 3 h, a reaction temperature of 61 ℃, and an acetic anhydride addition volume of 9.3 mL. Under these conditions, A-EMP-K exhibits a degree of substitution at 0.326. The A-EMP-K is a heteropolysaccharide consisting of galactose, arabinose, galacturonic acid, and other constituents. The absorption peaks of infrared spectra at 1 731 cm-1 and 1 257 cm-1 confirm the successful introduction of acetyl groups. Scanning electron microscope (SEM) results reveal a distinct porous structure and a relatively smooth surface. The particle size and zeta potential results indicate a relatively stable solvent system. With acarbose as the positive control, the inhibition rate of the A-EMP-K is (63.80±4.58)% against α-glucosidase, and is (81.18±2.48)% against α-amylase. [Conclusion] EMP-K and A-EMP-K exhibit potent performance in hypoglycemic activities.
Publication Date
9-20-2026
First Page
149
Last Page
158
DOI
10.13652/j.spjx.1003.5788.2025.80766
Recommended Citation
Xue, Xue; Hui, Tang; Bingwu, Liao; Lu, Chen; Wenbin, Zhao; Xinbing, Wang; Yan, Sun; and Youhong, Wei
(2026)
"Preparation , structural characterization , and in vitro hypoglycemic activities of acetylated Elaeagnus moorcroftii Wall . polysaccharides,"
Food and Machinery: Vol. 42:
Iss.
8, Article 19.
DOI: 10.13652/j.spjx.1003.5788.2025.80766
Available at:
https://www.ifoodmm.cn/journal/vol42/iss8/19
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