Abstract
[Objective] To establish a method based on ultrahigh performance liquid chromatography-tandem mass spectrometry for rapid determination of eight sweeteners in glycyrrhizin. [Methods] The sample is ultrasonically extracted with 20 mL pure water for 30 min, passed through a 0.22 μm microporous filter membrane, separated through the Waters HSS T3 column, subjected to gradient elution with 0.2% (V/V) ammonia solution-methanol, and detected under the negative mode of electrospray ionization. [Results] The eight sweeteners showed a good linear relationship between peak area and concentration within the range of 0.1–20.0 μg/mL, with correlation coefficients greater than 0.99. The limits of quantification are within the range of 0.2–1.0 μg/g. The average recoveries of samples spiked are within the range of 85.8%–103.0%, and the relative standard deviations are in the range of 2.4%–4.6%. [Conclusion] This method is simple, rapid, and suitable for determination of the content of eight sweeteners in glycyrrhizin.
Publication Date
9-20-2026
First Page
55
Last Page
60
DOI
10.13652/j.spjx.1003.5788.2025.80725
Recommended Citation
Liang, He; Lingchao, Zhu; Ruilin, Hu; Tao, Zhang; and Chunman, Song
(2026)
"Simultaneous determination of eight sweeteners in glycyrrhizin by ultrahigh performance liquid chromatograph-tandem mass spectrometry,"
Food and Machinery: Vol. 42:
Iss.
8, Article 8.
DOI: 10.13652/j.spjx.1003.5788.2025.80725
Available at:
https://www.ifoodmm.cn/journal/vol42/iss8/8
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