To investigate the distribution of serotypes, genetic relativity and epidemiological characteristic of Listeria monocytogenes (LM). 22 LM strains isolated from Guangzhou and Xiamen were investigated by ERIC-PCR and Sau-PCR. Results of ERIC-PCR showed that these strains were distributed in 8 patterns (a-h). The main pattern was pattern h, and the number of strains was 8. Genotyping using Sau-PCR showed that all tested strains were grouped into 7 clusters (A-G). These results indicated the genetic relativity and epidemiological characteristic of different LM food-borne isolates.

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[1] 闫鹤,王彬,师宝忠,等.单核细胞增生李斯特菌血清型、耐药性研究[J].中国抗生素杂志,2010,35(10):774~778
[2] 陈玉贞,侯配斌,贾静,等.2007年~2009年山东省生肉中单增李斯特菌的耐药性及分子分型分析[J].中国卫生检验杂志, 2012, 22(2): 267~269.
[3] 姜晓冰,石磊.单核细胞增生李斯特菌脉冲场凝胶电泳分型[J].食品与机械, 2009, 25(3): 84~86.
[4] Seong-Keun Byun,Suk Chan Jung,Han Sang Yoo. Random amplification of polymorphic DNA typing of Listetia monocytogenes isolated from meat[J]. International Journal of food Microbiology,2001,69(3):227~235.
[5] 毕水莲,李琳,石磊,等. Sau-PCR 和AILP技术对一起副溶血弧菌引起食物中毒的分型研究[J]. 食品工业科技,2009(9):158~161.
[6] Liu D Y,Lawrence M L,Gorski L,et al. Listetia monocytogenes serotype 4b strains belonging to lineages I and III possess distinct molecular features[J]. Journal of Clinical Microbiology,2006,44(1):214~217.
[7] Loessner M J. Improved procedure for bacteriophage typingof Listeria strains and evaluation of new phages[J]. Applied and Environmental Microbiology,1991,57(3):882~884.
[8] Graves L M,Swaminathan B,Reeves M W,et al. Comparison of ribotyping and multilocus enzyme electrophoresis for subtyping of Listeria monocytogenes isolates[J]. Journal of Clinical Microbiology,1994,32(12):2 936~2 943.
[9] M Tamburro, G Ripabelli, I Fanelli, et al.Typing of listeria monocytogenes strains isolated in Italy by inlA gene characterization and evaluation of a new cost-eddective approach to antisera for serotyping[J].Journal of Applied Microbiology,2009,4(1):1 602~1 609.
[10] Bang-Yuan Chen, Rajkumar Pyla, Rajkumar Pyla, et al. Prevalence and contamination patternsof listeria monocytogenes in catfish processing environment and fresh fillets [J].Food Microbiology,2010, 27(2): 645~652.
[11] Versalovic J,Koeuth T,Lupski J R. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes[J]. Nucleic Acids Research,1991,19(24):6 823~6 831.
[12] Burr M D,Josephson K L,Pepper I L. An evaluation of ERIC PCR and AP PCR fingerprinting for discriminating Salmonella serotypes[J]. Letters in Applied Microbiology,1998,27(1):24~30.
[13] 狄慧玲,石磊. 食源性沙门氏菌Sau-PCR基因分型研究[J]. 食品与机械,2008,24(3):87~89.
[14] 金莉莉,董雪,王秋雨,等. 沈阳市副溶血弧菌重复序列PCR分型[J]. 中国公共卫生,2008(3):351~353.
[15] 丁久法,潘迎捷,陈洪友,等. 副溶血性弧菌ERIC-PCR分型及毒力基因检测研究[J]. 食品工业科技,2010(8):137~141.
[16] B Jersek, P Gilot, M Gubina.Typing of listeria monocytogenes Strains by repetitive element sequence-based PCR [J].Journal of Clinical Microbiology, 1999, 37(1):103~109.
[17] Corich V,Mattiazzi A,Soldati E,et al. Sau-PCR,a novel ampli-fication technique for genetic fingerprinting of microorganisms[J]. Appl Environ Microbiol,2005,71(10): 6 401~6 406.
[18] 刘海泉,朱颖,姜文洁, 等.ERIC-PCR技术对单增李斯特菌的溯源分析[J]. 食品工业科技,2013,34(8):49~51.



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