To explore the Symbiotic and Epiphyte bacterium DNC2 from Strongylocentrotus intermedius, the morphological, biochemical and physiological characteristics of the strain were researched and the 16S rDNA sequences of it were analyzed. The univariate experiment and orthogonal test of L16(45) were carried out to optimize the fermentation conditions for antibacterial activity. Symbiotic and Epiphyte bacterium DNC2 was identified as Bacillus subtilis. The optimal fermentation conditions were obtained as fermentation at 35 ℃ for 120 h with initial pH 9, and the optimum fermentation medium was determined as beef extract 0.3% and peptone 0.6%. The optimized fermentation broth also showed good effect on inhibiting the growth of 8 kinds of fungus. The inhibition rate was 92% against Trichothecium roseum (Bull.) Link, 89% against Botrytis cinerea Pers and A.solani (Ell&Mart).sorauer, 88% against Ralstonia solanacearum, 81% against Mucor mucedo (L.)Brefeld, 73% against Fusarium oxysporum f.sp.niveum, 72% against Fusarium oxysporum of Cotton and 52% against Fusarium moniliformis.

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