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Abstract

Objective to optimize the technology for extracting process of total flavonoids from Ginkgo biloba leaves. Methods Based on the single factor experiments for ethanol concentration, solid-liquid ratio and extracting time, orthogonal experiment L9 (33) was designed to optimize the ethanol extracting conditions of total flavonoids by ultrasonic-assisted method. Results the optimal technology for extracting conditions of total flavonoids was as followed: with 60% ethanol concentration, 1∶50 (m∶V) solid-liquid ratio and 30 min of extracting time. Conclusion According to the results of variance analysis, ethanol concentration (P<0.01) had the greatest influence on yield of total flavonoids, followed by solid-liquid ratio (P<0.05), extracting time was the minimal influence (P>0.05). Under these conditions, the yield of total flavonoids was 8.075 mg/g and the extracting rate was 80.75%.

Publication Date

2-28-2015

First Page

167

Last Page

170

DOI

10.13652/j.issn.1003-5788.2015.01.039

References

[1] 易美华. 生物资源开发与加工技术[M]. 第一版. 北京:化学工业出版社,2009.
[2] 齐惠珍,周霞瑾,王明霞. 银杏叶提取物的药理作用及其临床应用研究进展[J]. 河北中医,2013,35(12):1 899~1 901.
[3] 张静. 银杏黄酮纯化及生物活性的研究[D]. 无锡:江南大学, 2010.
[4] 温啸, 贺大方, 倪学勤, 等. 干酪乳杆菌与银杏叶提取物对肥胖小鼠血脂及抗氧化功能的影响[J]. 西北农林科技大学学报(自然科学版), 2014, 42(1): 1~6.
[5] Tian Xiao-qiang, Zhang Li-da, Wang Jing-mei, et al. The protective effect of hyperbaric oxygen and Ginkgo biloba extract on Aβ25-35-induced oxidative stress and neuronal apoptosis in rats [J]. Behavioural Brain Research, 2013(242): 1~8.
[6] 冯金霞, 何玲, 蒲雪梅. 银杏叶提取液对鲜切红富士苹果品质的影响[J]. 食品科学, 2013, 34(20): 302~306.
[7] 王婵. 中草药植物提取液对鸡蛋涂膜保鲜效果的应用研究[D]. 长沙:华中农业大学, 2010.
[8] 刘爽, 付春旭, 刘学军. 银杏叶提取物对酱卤制品护色的研究[J]. 当代生态农业, 2012, 1(2): 113~117.
[9] 李虎, 徐奇, 李文静, 等. 银杏叶黄酮粗提物提取及其抑菌性质研究[J]. 中国酿造, 2011(11): 107~110.
[10] 李焰, 杨小燕, 林跃鑫, 等. 银杏叶有效成分提取与抑菌效果研究[J]. 中国畜牧杂志, 2006, 42(15): 54~56.
[11] 宋丽萍. 银杏叶总黄酮的提取工艺研究[J]. 海峡药学,2013,25(6):37~39.
[12] 毛火龙,王丽丽,熊英. 微波辅助法提取银杏叶总黄酮的研究[J]. 九江学院学报,2014(1): 78~81.
[13] 陈洪彬,郑金水,蔡英卿. 紫苏叶中总黄酮的超声波辅助提取工艺优化[J]. 食品与机械,2014, 30(5): 232~236.
[14] 王艳丽,何煜波,曹晓宁,等. 辣椒叶中总黄酮提取工艺的优化[J]. 食品与机械,2013, 29(3): 138~140, 168.
[15] 李峰,刘浩,钟媛,等. 沙棘叶中总多酚和总黄酮的提取工艺[J]. 食品与机械,2012, 28(4): 128~130.
[16] 张素斌,廖燕婷. 4种甜荞麦黄酮提取方法的比较研究[J]. 食品与机械,2012, 28(6): 150~153.
[17] 董周勇,任辉,周亚军. 松籽壳总黄酮超声提取工艺响应面优化[J]. 食品与机械,2011, 27(5): 100~102.
[18] 赖红芳, 黄秀香, 陆俊宇. 超声波辅助提取山豆根中的黄酮和多糖工艺优化[J]. 食品与机械, 2014, 30(1): 196~198, 223.
[19] 杨港坚,冯达星. 水溶性银杏总黄酮提取工艺研究[J]. 中医临床研究,2010, 2(3): 120~121.
[20] 刘海鹏,刘延成,马东升,等. 超声波/回流法银杏叶总黄酮提取的研究[J]. 化学工程师,2005(8): 49~50.

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