Abstract
A method for separation and purification of polyphenols from Walnut peel with macroporous resins. The results as follows: five kinds of macroporous resins were selected, AB-8 resin was considered as suitable material to purify and separate the Walnut peel polyphenols. The optimum conditions were concentration of feed 3.0 mg/mL at pH 4.0, and the volume of polyphenols sample 100 mL, flowing rate 2.0 mL/min, elute ethanol concentration 30%, ethanol at pH 6.0, elute volume 250 mL, elution rate 2.0 mL/min. After eluted with AB-8 resin, the polyphenols purity reach 36.36%, which is approximately 4.8 times higher than the previous7.65%.
Publication Date
2-28-2015
First Page
175
Last Page
180
DOI
10.13652/j.issn.1003-5788.2015.01.041
Recommended Citation
Qiong, LI; Kai, CHEN; Yanqin, CHEN; and Huanrong, LI
(2015)
"Purification of polyphenols from walnut peel by macroporous resin adsorption,"
Food and Machinery: Vol. 31:
Iss.
1, Article 41.
DOI: 10.13652/j.issn.1003-5788.2015.01.041
Available at:
https://www.ifoodmm.cn/journal/vol31/iss1/41
References
[1] 阿布力克木·阿布力孜，迪丽努尔·塔力甫，艾沙·努拉洪.分光光度法测定和田核桃仁内隔膜中总黄酮含量［J］.食品科学,2008,29(11): 552～554.
[2] 李志美.核桃的开发利用［J］.林业调查规划,2004(S1):199～201.
[3] 王晓泊,张翠,刘颖.青龙衣药用研究现状［J］.青龙衣药用研究现状,2007,28(3):120～122.
[4] 李海洋,韩军岐,李志西.核桃青皮有效化学成分提取分离研究综述［J］.现代园艺,2012(15):9～11.
[5] 刘丹. 亲水性多酚分离纯化技术研究［D］.大连:大连理工大学,2010.
[6] 赵元鸿,杨富佑,谢冰,等.茶多酚的制备及沉淀机理探讨［J］.云南大学学报,1999,21(4):317～318.
[7] 何晓梅,刘体云,魏梦洁.沉淀法制备茶多酚工艺研究［J］.安徽农业科学,2011,39(22):13 503～13 505.
[8] 韩丙军,彭黎旭.植物多酚提取技术及其开发应用现状［J］.华南热带农业大学学报,2005,11(1):21～26.
[9] 董新荣,刘仲华,李本祥,等.制备型高效液相色谱法制备纯化3种辣椒素单体［J］. 色谱,2008,26(3):366~369.
[10] 秦为辉,陈新,张长春,等.提取与分离新技术在天然产物研究中的应用［J］. 武汉工业学院学报,2008,27(2):19~22,31.
[11] 岳敏.大孔吸附树脂在富集分离中药有效部位工艺中的应用［D］.北京:首都师范大学,2004.
[12] 董晓燕.生物化学实验［M］.北京:化学工业出版社,2003.
[13] 陈亮,李医明,陈凯先,等.植物多酚类成分提取分离研究进展［J］.中草药,2013,44(11):1 501～1 507.
[14] 张赟彬,李彩侠,吴亚卿.黄酮类化合物的研究进展［J］.食品与机械,2005,21(5):77～80.
[15] 张晓丽,曾晓雄,李春阳. 菜籽多酚提取工艺的优化［J］.食品与机械,2012,28(2):142～146.
[16] Ivo O, Anabela S, Isabel C F R, et al. Total phenols, antioxidant potential and antimicrobial activity of walnut (Juglans regia L.) green husks［J］. Food and Chemical Toxicology,2008, 46(1): 2 326～2 331.
[17] 曹增梅.番石榴多酚的提取分离及保鲜应用研究［D］.湛江:广东海洋大学,2013.
[18] 艾志录,王育红,王海,等.大孔树脂对苹果渣中多酚物质的吸附研究［J］.农业工程学报,2007,23(8):245～248.
[19] 袁英姿,曹清明,钟海雁,等.大孔吸附树脂纯化油茶籽多酚的研究［J］.食品与机械, 2009,25(1):61～63,85.
[20] 李志洲. 微波辅助提取石榴叶中多酚类物质的工艺［J］.食品与机械,2009,25(4):72～75.
[21] 周跃勇,王岸娜,吴立根.大孔吸附树脂分离纯化猕猴桃中多酚的优选研究［J］.河南工业大学学报(自然科学版),2007,28(5):32～36.