Study on quantum dot immune chromatography test strip for Escherichia coli O157:H7

Authors

YUAN Liejiang, Institute of Product Quality Supervision and Inspection of Hunan Province, Changsha, Hunan 410007, China
LI Mengli, School of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
WANG Shuyuan, School of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
YANG Mengxin, School of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
LI Zhonghai, School of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China

Abstract

To develop a quantum dotsb(QDs) immune chromatographic strip test for rapid and sensitive detection of Escherichia coli O157:H7. Water soluble QDs was electrostatic coupled with mouse anti- Escherichia coli O157:H7 monoclonal antibody and mouse anti- Escherichia coli O157:H7 and rabbit anti-mouse IgG were marking on the NC membrane as test line and control line, respectively. The rapid detection of immune chromatographic strip test paper was based on double antibody sandwich. With the immune chromatographic strip test, only 5 min was needed for detection. The limit of detection was 1×10⁴ CFU/mL. No cross-reactivity was found with 8 kinds of common food borne pathogens. The QDs immune chromatographic strip test offers a rapid and sensitive tool for the detection of Escherichia coli O157:H7

Publication Date

8-28-2015

First Page

38

Last Page

42

DOI

10.13652/j.issn.1003-5788.2015.04.010

Recommended Citation

Liejiang, YUAN; Mengli, LI; Shuyuan, WANG; Mengxin, YANG; and Zhonghai, LI (2015) "Study on quantum dot immune chromatography test strip for Escherichia coli O157:H7," Food and Machinery: Vol. 31: Iss. 4, Article 10.
DOI: 10.13652/j.issn.1003-5788.2015.04.010
Available at: https://www.ifoodmm.cn/journal/vol31/iss4/10

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