Polysaccharide yield was as index, and optimal ultrasonic-assisted extraction process of polysaccharides from sporophyll of Undaria pinnatifida was investigated by Box-Behnken design based on single factor tests. Meanwhile, the antioxidant activity of the polysaccharide was also studied by electron spin resonance (ESR) technology. It turned out that the optimal extraction conditions were as follow: ratio of water to material was 90︰1 (V∶m), ultrasonic time was 6.5 min and ultrasonic power was 440 W, extraction time was 2 h, respectively. Polysaccharide yield achieved 7.71% under that condition. And the polysaccharide from sporophyll of Undaria pinnatifida gave potent scavenging activity against DPPH· and ·OH, the IC50 were 0.24 mg/mL and 0.28 mg/mL, respectively.

Publication Date


First Page


Last Page





[1] Lee J, Hayashi K, Hashimoto M, et al. Novel antiviral fucoidan from sporophyll of Undaria pinnatifida (Mekabu)[J]. Chemical & Pharmaceutical Bulletin, 2004, 52(9): 1 091~1 094.
[2] 农业部渔业局.2014年中国渔业统计年鉴[Z].北京:中国农业出版社,2014:34.
[3] Katsube T, Yamasaki Y, Iwamoto M, et al. Hyaluronidase-inhibiting polysaccharide isolated and purified from hot water extract of sporophyll of Undaria pinnatifida[J]. Food Science and Technology Research, 2003, 9(1): 25~29.
[4] 苏秀榕,李太武,丁明进.裙带菜孢子叶营养成分分析[J].营养学报,1994,16(2):236~238.
[5] Synytsya A, Kim W, Kim S, et al. Structure and antitumour activity of fucoidan isolated from sporophyll of Korean brown seaweed Undaria pinnatifida[J]. Carbohydrate Polymers, 2010, 81(1): 41~48.
[6] 洪胜,潘利华,罗建平.生物多糖超声波辅助提取研究进展[J].食品工业科技,2011,32(8):481~484.
[7] 朱良,张青,朱钦昌,等.正交设计优化裙带菜孢子叶多糖提取工艺[J].中国海洋药物杂志,2006,25(6):42~45.
[8] 余洋定,启航,李冬梅,等.果胶酶辅助提取裙带菜孢子叶多糖的工艺条件优化[J].食品与机械,2012,28(1):175~177.
[9] 张警予,李裕博,余洋定,等.纤维素酶辅助提取裙带菜孢子叶多糖工艺条件优化的研究[J].食品科技,2014,39(8):178~181.
[10] 李宏睿,范琳琳,徐明生,等.苦瓜多糖超声波辅助提取工艺优化[J].食品与机械,2010,26(2):107~125.
[11] 胡爱军,郑捷. 食品工业中的超声提取技术[J].食品与机械, 2004,20(8): 57~60.
[12] Qi Hang, Yu Yang-ding, Sun Li-ming, et al. Purification and partial bioactivity in vitro of polysaccharides from sporophyll of Undaria pinnatifida[J]. Journal of Food, Agriculture & Environment, 2012, 10(3&4): 197~201.
[13] 李建,杨波,季宇彬,等.超声波提取裙带菜多糖的工艺研究[J].食品与药品,2010,12(11):404~406.
[14] 赵雅娉,刘山,赵鑫,等.黄海海燕体壁胶原蛋白肽的制备及其抗氧化活性[J].食品研究与开发,2013, 35(1):5~8.
[15] 奚倩,赵雅娉,张警予,等.利用电子自旋共振技术研究海参提取液体外抗氧化活性[J].食品与机械,2014, 30(5):36~40.
[16] 李裕博,纪晓琳,刘山,等.黄海海燕体壁酶解物抗氧化活性研究[J].食品与机械,2015,31(1):137~140.
[17] 李坚斌,李琳,李冰,等.超声降解多糖研究进展[J].食品工业科技,2006,27(9):181~184.
[18] Zhong Kai, Wang Qiang. Optimization of ultrasonic extraction of polysaccharides from dried longan pulp using response surface methodology[J]. Carbohydrate Polymers, 2010, 80(1):19~25.
[19] 赵保路.电子自旋共振技术在生物和医学中的应用[M].合肥:中国科学技术大学出版社,2009:58~59.
[20] Falch E, Velasco J, Aursand M, et al. Detection of radical development by ESR spectroscopy techniques for assessment of oxidative susceptibility of fish oils[J]. European Food Research and Technology. A, 2005, 221(5): 667~674.
[21] Lee S J, Cheong S H, Kim Y S, et al. Antioxidant activity of a novel synthetic hexa-peptide derived from an enzymatic hydrolysate of duck skin by-products[J]. Food and Chemical Toxicology, 2013(62): 276~280.
[22] 何传波,魏好程,熊何健,等.酶与微波处理对海带多糖提取及抗氧化活性的影响[J].食品科学,2013,34(18):51~53.

Included in

Food Science Commons



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.