The combination technology of membrane separation technology and macroporous resin purification technology have been adopted to separate and purify tea saponin. Tea saponin extractive was preliminarily purified and concentrated by flowing through ceramic membrane and 360 Da nanofiltration membrane in sequence, the yield and purity of tea saponin is 62.1% and 79%, respectively. On the basis of static adsorption and desorption as well as dynamic adsorption and desorption experiments, the macroporous resin AmberliteXAD7HP was selected for further purification. the The optimum conditions for purification of tea saponin using macroporous resin were established by single factor tests as follows: the sample flow rate of 0.5 mL/min, the sample concentration of 30 mg/mL; gradient elution with ethanol of different concentration, 10%, 40% and 70% respectively, flow rate of 1 mL/min, elution volume was 3 BV. Under these optimum conditions, the final yield of tea saponin was 55.3% with the purity of 95%. High purity tea saponin has been got, and indicates that membrane separation and macroporous resin combination technology is a feasible method of industrialization.

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[1] 侯如燕. 茶皂素提取纯化及其抗菌活性研究[D]. 合肥: 安徽农业大学, 2002.
[2] Sparg S G, Light M E, Van Staden J. Biological activities and distribution of plant saponins[J]. Journal of Ethnopharmacology, 2004, 94(2): 219~243.
[3] Jin Feng, Chen Ying, Liu Xin, et al. Efficient improvement of surface activity of tea saponin through Gemini-like modification by straightforward esterification[J]. Food Chemistry, 2015, 171: 272~279.
[4] 刘蓉, 张利蕾, 范亚苇, 等. 茶渣中粗茶皂素的纯化及其抗氧化和抑菌活性[J]. 南昌大学学报(工科版), 2013,35(1): 17~21.
[5] 李祥, 曹江绒, 种亚莉. 正丁醇萃取茶皂素的研究[J]. 陕西科技大学学报(自然科学版), 2014, 32(1): 87~90.
[6] 赵娟, 黄健花, 蔡春明, 等. 沉淀法纯化茶皂素的工艺研究[J]. 中国油脂, 2010,35(11): 58~61.
[7] Chen Junghui, Wu Hsingyu, Liau Bingchung, et al. Identification and evaluation of antioxidants defatted Camellia oleifera seeds by isopropanol salting-out pretreatment[J]. Food Chemistry, 2010, 121(4): 1 246~1 254.
[8] 涂云飞, 杜先锋, 张雪波. 干柱层析法制备油茶总皂苷对照品初探[J]. 中国农学通报, 2006, 22(10): 343~345.
[9] Yang Peng-jie, Zhou Ming-da, Zhou Cheng-yun, et al. Separation and purification of both tea seed polysaccharide and saponin from camellia cake extract using macroporous resin[J]. Journal of Separation Science, 2015, 38(4):656~662.
[10] 程轶群, 赵敬娟, 夏涛, 等. 茶皂素的陶瓷膜纯化工艺优化及脱色研究[J]. 农产品加工(学刊), 2011(11): 11~15.
[11] 韩晓彤, 库海福, 李霞, 等. 大孔树脂 HPD400 分离茶皂素的研究[J]. 粮油食品科技, 2014, 22(6): 39~43.
[12] 曹江绒. 茶皂素的提取、纯化及在洗发液中的应用研究[D]. 西安: 陕西科技大学, 2014.
[13] 吴丽, 黄福平. 茶籽中茶皂素的分离纯化及脱色工艺研究进展[J]. 南方林业科学, 2015(1): 32~35.
[14] 胡婕伦, 聂少平, 龚毅, 等. 响应曲面法优化茶皂素提取工艺的研究[J]. 食品科学, 2009,30(18): 106~109.
[15] 张海龙. 冷榨茶籽饼中茶皂素的提取纯化及鉴定[D]. 武汉: 武汉工业学院,2012.
[16] 郭辉力, 邓泽元, 彭游, 等. 微波/光波辅助提取茶皂素的研究[J]. 食品工业科技, 2008,29(11): 168~170.
[17] 张新富. 油茶皂苷分离纯化及生物活性研究[D]. 合肥: 安徽农业大学,2013.
[18] 赵敬娟. 油茶皂苷的提取及膜分离纯化的研究[D]. 合肥: 安徽农业大学,2009.
[19] 贾存勤, 李阳春, 屠鹏飞, 等. HPD 系列大孔吸附树脂预处理方法研究[J]. 中国中药杂志, 2006, 30(18): 1 425~1 427.
[20] Rigano L, Lionetti N, Otero R. Quillaja triterpenic saponins-the natural foamers[J]. SOFW-Journal, 2009, 135(4): 1~9.
[21] Buran T J, Sandhu A K, Li Zheng, et al. Adsorption/desorption characteristics and separation of anthocyanins and polyphenols from blueberries using macroporous adsorbent resins[J]. Journal of Food Engineering, 2014, 128(1): 167~173.
[22] 孟维, 李湘洲, 吴志平, 等. AB-8 型大孔吸附树脂分离纯化茶皂素工艺[J]. 食品科技, 2013, 38(9): 213~217.



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