Abstract
Ara h 2 is one of the major allergen in peanut. Raw peanut was subjected to a series of sequential treatments, such as protein extraction, DEAE-Sepharose Fast Flow anion-exchange chromatography and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS—PAGE) recovery. The purified protein was identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI—TOF/MS). The results showed that the highly purified protein could be obtained by this method and was identified as Ara h 2 by MALDI—TOF/MS. The molecular weights of two constituents were 18.5 kD (Ara h 2.01) and 20.1 kD (Ara h 2.02). Recovery rate of column chromatography separation and SDS—PAGE electrophoresis were 31.4% and 18.6%, respectively.
Publication Date
8-28-2015
First Page
27
Last Page
30
DOI
10.13652/j.issn.1003-5788.2015.04.007
Recommended Citation
Qingqing, ZHU; Wenju, ZHANG; Qin, CAI; and Qin, CHEN
(2015)
"Purification and identification of endogenous allergy protein Ara h 2 in peanut,"
Food and Machinery: Vol. 31:
Iss.
4, Article 7.
DOI: 10.13652/j.issn.1003-5788.2015.04.007
Available at:
https://www.ifoodmm.cn/journal/vol31/iss4/7
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