With the soy protein isolated(SPI) and zein as the raw material, through modified the zein by microwave to make the microwave modified blended protein film. Selecting the protein liquid volume ratio of SPI and modified zein, ethanol content, pH of film-forming liquid and water bath temperature as studying factors, the mechanical properties of blended film (breaking strength and fracturing distance) as targets for single factor experiment was studied. And the microstructure, thermal characteristics and FT—IR of the microwave modified blended protein film were also studied, which was obtained by the best ultrasonic film-forming conditions. The results showed that the blended film had the maximal breaking strength (2 900.00 g) and the highest fracturing distance (16.08 mm), with the protein liquid volume ratio of SPI and modified zein 3∶1, the ethanol content 20%, the pH of film-forming liquid 12 and the water bath temperature 80 ℃. The blended film has the better microstructure and thermal stability, the closer intermolecular interactions, and the typical characteristics of secondary structure.

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[1] 王海鸥. 可食性膜及其在食品工业中的应用[J].食品与机械,2002(5): 4~8.
[2] 蔡花真,徐艳艳,李梦琴,等. 可食性复合蛋白成膜条件研究[J].食品与机械,2010,26(5): 112~114.
[3] Chen Ye, Ye Ran, Li Xiu-ming, et al. Preparation and characterization of extruded thermoplastic zein poly (propylene carbonate) film[J]. Industrial Crops and Products, 2013(49): 81~87.
[4] Biswasa Atanu, Selling G W, Woods K K, et al. Surface modification of zein films[J]. Industrial Crops and Products, 2009, 30(1): 168~171.
[5] Shi Ke, Huang Yu-ping, Yu Hai-long, et al. Reducing the brittleness of zein films through chemical modification[J]. J. Agric. Food Chem., 2011, 59(1): 56~61.
[6] Ghanbarzadeh B, Oromiehie A, Musavi M. Effect of plasticizing sugars on rheological and thermal properties of zein resins and mechanical properties of zein films[J]. Food Research International, 2006, 39(8): 882~890.
[7] Guo H X, Heinmki J, Yliruusi J. Stable aqueous film coating dispersion of zein[J]. Journal of Colloid and Interface Science, 2008, 322(2): 478~484.
[8] 刘少博,陈复生,刘昆仑,等. 胶原蛋白与大豆分离蛋白复合膜制备的研究[J].食品与机械,2014,30(4): 107~112.
[9] Cunningham P, Ogale A A, Dawson P L, et al. Tensile properties of soy protein isolate films produced by a thermal compaction technique[J]. Journal of Food Science, 2000, 65(4): 668~671.
[10] Swain S N, Biswal S M, Nanda P K, et al. Biodegradable soy-based plastics: opportunities and challenges[J].Journal of Polymers and the Environment, 2004, 12(1): 35~42.
[11] Zubov V P, Chikhacheva I P, Nikolaeva E I, et al. Microwave-assisted synthesis of composite sorbents on the basis of silica modified by polyvinyl alcohol[J]. Russian Journal of General Chemistry, 2009, 79(2): 191~194.
[12] Li Ji, Zu Yuan-gang, Luo Meng, et al. Aqueous enzymatic process assisted by microwave extraction of oil from yellow horn (Xanthoceras sorbifolia Bunge.) seed kernels and its quality evaluation[J]. Food Chemistry, 2013, 138(4): 2 152~2 158.
[13] 张德欣,李治龙,刘新华.微波处理对提高谷朊粉品质的影响[J].农产品加工,2010(6):45~49.
[14] 陈剑兵, 夏其乐, 张俊, 等. 微波技术对大豆蛋白功能特性的影响[J].保鲜与加工,2007,7(4):33~35.
[15] Copty A B, Neve O Y, Barak I, et al. Evidence for a specific microwave radiation effect on the green fluorescent protein[J]. Biophysical Journal, 2006, 91(4): 1 413~1 423.
[16] 顾璐萍. 食品蛋白质的性质对其成膜性能的影响[D].无锡:江南大学,2013.
[17] 李超,李强,李梦琴,等.制膜条件对可食性小麦面筋蛋白膜机械性能的影响[J].广州食品工业科技,2004,20(4): 8~11.
[18] 贾祥祥,郭兴凤,鲁亚楠,等. 制备条件对玉米醇溶蛋白膜机械性能的影响[J]. 粮食与饲料工业,2012(7): 36~40.
[19] 艾志录, 李梦芹, 张平安, 等,小麦面筋蛋白膜的研制及其耐湿性的改善[J].中国食品学报,2003,3(4): 56~61.
[20] 唐亚丽,赵伟,卢立新,等. 增塑剂浓度和干燥温度对大豆蛋白膜阻油性能与机械性能的影响[J]. 安徽农业科学,2011, 39(30): 378~379.
[21] Wang Zhe, Sun Xiu-xiu, Lian Zi-xuan, et al. The effects of ultrasonic/microwave assisted treatment on the properties of soy protein isolate/microcrystalline wheat-bran cellulose film[J]. Journal of Food Engineering, 2013, 114(2): 183~191.
[22] 黄国平. 玉米醇溶蛋白的超声波提取、改性与释药性能的研究[D].广州:华南理工大学,2004.
[23] 姜燕,唐传核,温其标. 大豆分离蛋白膜的DSC分析[J].食品与发酵工业,2008,34(1): 28~30.
[24] Kokoszka S, Debeaufort F, Hambleton A, et al. Protein and glycerol contents affect physico-chemical properties of soy protein isolate-based edible films[J]. Innovative Food Science & Emerging Technologies,2010, 11(3): 503~510.
[25] 刘海飞,肖志刚,王娜,等.稳态化米糠蛋白超声波辅助提取及其性质研究[J].农业机械学报,2014,45(11): 235~241.
[26] 卢雁,张玮玮,王公轲. FTIR用于变性蛋白质二级结构的研究进展[J].光谱学与光谱分析,2008,28(1): 88~93.
[27] Gu Lu-ping, Wang Miao, Zhou Jing-wen. Effects of protein interactions on properties and microstructure of zein-gliadin composite films[J]. Journal of Food Engineering, 2013, 119(2): 288~298.
[28] Surewicz W K, Mantsch H H, Chapman D. Determination of protein secondary structure by Fourier transform infrared spectroscopy:a critical assessment[J]. Biochemistry, 1993, 32(2): 389~394.
[29] Schmidt V, Giacomelli C, Soldi V. Thermal stability of films formed by soy protein isolate-sodium dodecyl sulfate[J]. Polymer Degradation and Stability, 2005, 87(1): 25~31.
[30] 江艳,武培怡. 大豆蛋白的中红外和近红外光谱研究[J].化学进展,2009, 21(4):705~714.



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