Preparation,separation and amino acid sequence analysis of corn oligopeptides with antialcoholism activity
Corn oligopeptides(COP) were prepared via enzymatic hydrolysis of destarched corn proteins by Alcalase 2.4L. By investigated COP antialcoholism activity in vitro and in vivo, it is found that COP has highly antialcoholism activity. Meanwhile, the scavenging rate of blood alcohol concentration is positively correlated with the dose of COP. COP were separated by Sephadex G-15 gel chromatogram and RP—HPLC step by step, then fraction COPⅢ-17 was separated with highly antialcoholism activity. UPLC—Q—TOF—MS analysis showed that its amino acid sequence: P-Y-L-P-L-L-P-S.
Wei, MA; Jun, DAI; Shangwei, CHEN; and Song, ZHU
"Preparation,separation and amino acid sequence analysis of corn oligopeptides with antialcoholism activity,"
Food and Machinery: Vol. 31:
5, Article 51.
Available at: https://www.ifoodmm.cn/journal/vol31/iss5/51
 Yamaguchi M, Takada M, Nozaki O, et al. Preparation of cornpeptide from corn gluten meal and its administration effect on alcohol metabolism in stroke-prone spontaneously hypertensive rats[J]. Journal of Nutritional Science and Vitaminology, 1996, 42(3): 219～231.
 Yamaguchi M, Takeuchi M, Ebihara K. Inhibitory effect of peptide prepared from corn gluten meal on 7,12-dimethylbenz[a] anthracene-induced mammary tumor progression in rats[J]. Nutrition Research, 1997, 17(7): 1 121～1 130.
 Yamaguchi M, Nishikiori F, Ito M, et al. The effects of corn peptide ingestion on facilitating alcohol metabolism in healthy men[J]. Bioscience, Biotechnology and Biochemistry, 1997, 61(9): 1 474～1 481.
 Zhang Feng, Zhang Jia-li, Li Yong. Corn oligopeptides protect against early alcoholic liver injury in rats[J]. Food and Chemical Toxicology, 2012, 50(6): 2 149～2 154.
 Yu Guo-cai, Lv Jie, He Hui, et al. Hepatoprotective effects of corn peptides against carbon tetrachloride-induced liver injury in mice[J]. Journal of Food Biochemistry, 2012, 36(4): 458～464.
 李晶. 玉米肽抗疲劳作用的实验研究[J]. 食品与机械, 2004, 20(1): 11～12.
 Li Jiang-tao, Zhang Jiu-liang, He Hui, et al. Apoptosis in human hepatoma HepG2 cells induced by corn peptides and its anti-tumor efficacy in H22 tumor bearing mice[J]. Food and Chemical Toxicology, 2013(51): 297～305.
 Yang Yan-jun, Tao Guan-jun, Liu Ping, et al. Peptide with anglotensin I-Converting enzyme inhibitory activity from hydrolyzed corn gluten meal[J]. Journal of Agricultural and Food Chemistry, 2007, 55(19): 7 891～7 895.
 Ma Zhi-li, Zhang Wen-jun, Yu Guo-cai, et al. The primary structure identification of a corn peptide facilitating alcohol metabolism by HPLC—MS/MS[J]. Peptides, 2012, 37(1): 138～143.
 Madhusudhan M C, Raghavarao K S M S, Sanjay Nene. Integrated process for extraction and purification of alcohol dehydrogenase from Baker s yeast involving precipitation and aqueous two phase extraction[J]. Biochemical Engineering Journal, 2008, 38(3): 414～420.
 隋玉杰. 玉米肽的制备条件及其醒酒护肝机理研究[D]. 武汉: 华中农业大学, 2006.