Separation and enrichment of polyphenols from sunflower kernel with mcroporous resin

Authors

XU Dandan, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China
ZHANG Wenbin, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China;State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China
YANG Ruijin, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China;State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China
ZHAO Wei, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China;State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China
HUA Xiao, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China;State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China

Abstract

An efficient preparative separation and enrichment process of polyphenols from sunflower kernel extracted by acid solution has been developed in the present study. 3-O-caffeoylquinic acid (CQA), 4-O-CQA and 5-O-CQA were identified as the main polyphenols in crude extract. The adsorption capacity and desorption ratio for total polyphenols of nine macroporous resins had been evaluated and XDA-1 resin was found to be the most suitable resin for the separation of polyphenols. It was found that ultrafiltration as preliminary treatment could improve the purity of crude extract from 4% to 15%. After that, the extract was submitted to XDA-1 resin column. The optimized purification conditions were obtained as following: polyphenol concentration in sample solution of 2.0 mg/mL, sample pH of 3.0, flow rate of 2 bed volume (BV)/h, sample volume of 30 BV. When the adsorption is done, 10% (V/V) ethanol was applied to the column to wipe off the impurity at 4 BV/h. Then polyphenols were eluted with 50% (V/V) ethanol at 4 BV/h. With the enrichment on XDA-1 resin, the purity of polyphenol in the final product was increased from 15% to 77%, and recovery yield of 82.3% was obtained. Therefore, combination of ultrafiltration and macroporous resin separation provided an efficient and cost-effective method for recovery of CQAs from sunflower kernel extract.

Publication Date

10-28-2015

First Page

210

Last Page

216

DOI

10.13652/j.issn.1003-5788.2015.05.054

Recommended Citation

Dandan, XU; Wenbin, ZHANG; Ruijin, YANG; Wei, ZHAO; and Xiao, HUA (2015) "Separation and enrichment of polyphenols from sunflower kernel with mcroporous resin," Food and Machinery: Vol. 31: Iss. 5, Article 54.
DOI: 10.13652/j.issn.1003-5788.2015.05.054
Available at: https://www.ifoodmm.cn/journal/vol31/iss5/54

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