•  
  •  
 

Abstract

The optimal enzymolysis processing for Onchidium struma muscle protein extraction by trypsin was investigated by response surface methodology and orthogonal design on the basis on the basis of single factor. The results showed that the optimum enzymolysis processing conditions via response surface methodology were as followed: enzymolysis temperature 45 ℃, enzymolysis time 2.3 h, pH value 9.0 and liquid-solid rate 92︰1(V︰m). And by the Orthogonal design, were enzymolysis temperature 45 ℃, enzymolysis time 2 h, pH value 9.0 and liquid-solid rate 90︰1(V︰m). The extraction protein rates by these of two methods were 8.74% and 8.52% respectively.

Publication Date

10-28-2015

First Page

226

Last Page

230

DOI

10.13652/j.issn.1003-5788.2015.05.057

References

[1] 孙变娜,沈和定. 浅议瘤背石磺酶解多肽开发前景[J]. 生物技术世界,2013(8):2.
[2] 管菊,沈和定,钱静,等. 四种石磺营养成分分析及价值评价[J]. 食品工业科技,2013(17):349~353,364.
[3] 刁亚,沈和定,程知庆,等. 响应面法优化胃蛋白酶酶解瘤背石磺肌肉蛋白工艺参数[J]. 安徽农业大学学报,2015(3):391~397.
[4] 孙变娜,沈和定,吴洪喜,等. 石磺营养价值、活性物质的研究现状及开发前景[J]. 江苏农业科学,2013(8):14~17.
[5] 张媛溶. 上海沿海蛤蟆石磺的初步研究[C]//贝类学会论文集第二辑.北京:北京科学出版社,1986:153.
[6] 李八方. 海洋生物活性物质[M]. 青岛:中国海洋大学出版社,2007.
[7] Khodabandeh S,Maleki D,Abdolzadeh M. Isolation of a 320 kDa protein from the eggs of Onchidium(sea slug) that exhibits anticancer activity against breast cancer cell line(T47D)[J]. The Breast,2011(20):18~19.
[8] 范东斌,莫弦丰,陈玉竹,等. 响应曲面法优化油茶饼粕蛋白的超声辅助提取工艺研究[J].食品工业科技,2014(3): 199~202.
[9] 郭显荣,黄卫文,龚吉军,等. 不同提油方法的油茶粕蛋白制备工艺研究[J]. 食品与机械,2013,29(6):147~149,175.
[10] 马秀婷,肖志刚,孙旭,等. 超声波辅助提取豆渣蛋白工艺优化[J]. 食品与机械,2013,29(1):108~112.
[11] 黄菊,翟淑敏. 正交设计和响应面法优化荞麦中芦丁提取工艺的比较[J]. 粮食与油脂,2014(9):45~48.
[12] 鲁宁. 鸡胸软骨胶原蛋白制备工艺研究[D]. 合肥:安徽农业大学,2013.
[13] 曹文红,冯思涛,莫敏婷,等. 青鳞鱼酶解工艺正交优化及其产物分子量分布的研究[J]. 食品科技,2013(4):130~135.
[14] 王永菲,王成国. 响应面法的理论与应用[J]. 中央民族大学学报(自然科学版),2005(3):236~240.
[15] 张艳,李永哲. 响应面法及其在药学领域中的应用[J]. 吉林化工学院学报,2012(7):20~26.
[16] Myers R H,Khuri A I. Response surface methodology:1966~1988[J]. Technometrics,1966,31(2):137~157.
[17] 徐向英,王岸娜,林伟静,等. 响应面法优化燕麦全粉中蛋白质提取工艺[J]. 食品与机械,2011,27(5):96~99.
[18] 李加兴,房惠芳,陈选,等. 牡丹籽粕蛋白提取工艺优化及其等电点分析[J]. 食品与机械,2014,30(3):147~150.
[19] 刘立新. 猪骨蛋白水提工艺的优化[J]. 食品与机械,2013,29(5):220~222.
[20] 王镜岩. 生物化学[M]. 北京:高等教育出版社,2007.
[21] 姚笛,马萍,王颖,等. 响应面法优化玉米芯中木聚糖的提取工艺[J]. 食品科学,2011(8):111~115.
[22] 邹华蓉. 棉籽水酶法制备油脂和蛋白质的工艺研究[D]. 武汉:湖北工业大学,2014.
[23] 胡耀辉. 食品生物化学[M]. 北京:化学工业出版社:2009.
[24] 何士敏,方平,郭利佳. 鱼腥草抗氧化成份的研究[J]. 西南农业学报,2009(3):625~631.
[25] 孙雪萍,杨家林,徐艳,等. 方格星虫蛋白酶解工艺优化[J]. 食品与机械,2015,31(2):238~242.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.