Three wheat varieties were selected as experimental materials to measure the activity of polyphenol oxidase According to the method from Measurement of Polyohenol Oxidase Activity in Wheat Flour in national standard draft. And found it has three deficiencies: first, because of the lack of crude extcation of polyphenol oxidase, it led to the less quantity of polyohenol oxidase, and couldn’t reflect the real state of the activity of polyphenol oxidase; second, because of the unsuitable reaction temperature, it couldn’t help the enzyme reaction completely; third, adding catechol before centrifuge made a part of product intercepted by precipitation, thus it has poor stability and exhibited small activity of polyphenol oxidase. Based on this method, explored the influences of different factors on the determination of activity of the polyphenol oxidase to optimize the method, including the extraction time, the heat temperature, and the time for adding substrate. The results revealed of the optimum method seems to be better than before, the optimum process were: extraction 4 h, add catechol after centrifuge, heat at 70 ℃ in 15 min, and could get higher activity of polyphenol oxidase and had better repeatability, it was more reliable and stable. Also detected 50 wheat varieties by these two methods, and divided special noodles flour into different level by the activity of polyphenol oxidase.

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[1] 魏益民. 谷物品质与食品加工[M]. 北京: 中国农业科学技术出版, 2005: 7~9.
[2] 刘宏, 汪丽萍, 刘明, 等. 稳定化全麦粉的品质评价[J]. 食品与机械, 2012, 28(2): 6~8.
[3] 张丹. 小麦制品色泽与多酚氧化酶特性研究[D]. 郑州: 河南工业大学, 2012: 25~32.
[4] 唐忠. 小麦粉及其制品添加剂的应用[J]. 食品与机械, 1995(1): 86~89.
[5] Svihus B, Uhlen A K, Harstad. Effect of starch granule structure associated components and processing on nutritive value of cereal starch[J]. Animal Feed Science and Technology, 2005, 122(3/4): 303~320.
[6] 葛秀秀. 中国面条颜色及其影响因素研究[D]. 北京: 中国农业科学院作物所, 2003: 23~24.
[7] 王培慧. 面粉、面片色泽影响因素的研究[D]. 郑州: 河南工业大学, 2012: 19~20.
[8] 司红起, 马传喜, 王伟. 小麦多酚氧化酶酶学特性的研究[J]. 粮食饲料与工业, 2008(5): 5~6, 23.
[9] Lamkin W M, Miller B S, Nelson S W, et al. Polyphenol oxidase activities of Hard Red Winter, Soft Red Winter, Hard Red Spring, White Common, Club, and Durum wheat cultivars[J]. Cereal Chemistry, 1981, 58(1): 27~31.
[10] 全国粮油标准化技术委员会.《粮油检验 小麦粉多酚氧化酶测定》国家标准征求意见稿[S]. 北京: 中国标准出版社, 2013.



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