HPLC and GC—MC methods determining several preservatives in injecta was studied. When HPLC method was conducted, with conditions of streaking under 230 nm wavelength for VWD UV detection and 50︰50 proportion of methanol and water in the mobile phase, the peak with obvious trailing were undesirable. while, using GC—MS method, the response value and separation efficiency were better than that of HPLC and the preservatives were easy to be qualitatively and quantitatively determined. Results: It was quick and easy to determine peak occurrence and separation effects preservatives in injecta with the GC—MS method. The linearity of peak area and concentrations for preservative components was good (r>0.994). The recovery rate of sample loading was 85.06%~88.98%. Therefore, GC—MS method is an ideal method to determine contents of various preservatives components in injecta, which is more sensitive, easier to operate, more accuracy, more reproducible, and simpler to prepare samples than HPLC method.

Publication Date


First Page


Last Page





[1] 陈曦, 刘美欣. 眼用制剂中防腐剂的研究应用进展[J]. 天津药学, 2013, 25(5): 54~58.
[2] 杨秀丽, 蒲道俊, 余春梅, 等. 高效液相色谱法测定多西他注射液的含量和有关物质[J]. 中国医药指南, 2012(18): 48~52.
[3] 李中东, 张云轩, 郭燕萍, 等. 羟苯酯类防腐剂的不良反应[J]. 中国药物警戒, 2012, 9(5): 303~305.
[4] 左磊, 周峰. 食品防腐剂ADI制定及正确使用对人体健康的理论探讨[J]. 中国卫生检验杂志, 2013, 23(8): 2 016~2 018.
[5] 许秀敏, 吴西梅, 梁春穗, 等. 液相色谱—质谱联用检测食品中苯甲酸、山梨酸、糖精钠[J]. 中国卫生检验杂志, 2005, 9(15): 1 057~1 059.
[6] 康绍英, 张继红, 沈汇琴, 等. 二极管阵列—高效液相色谱同时检测食品中的安赛蜜苯甲酸山梨酸糖精钠和脱氢乙酸[J]. 食品与机械, 2007, 23(5): 118~121.
[7] 王祖翔, 周洪斌, 蒋俊, 等. 气相色谱-串联质谱法测定复合调味料中的微量苯甲酸[J]. 食品与机械, 2011, 27(3): 84~87.
[8] 桑宏庆, 蔡华珍, 王大勇. 紫外分光光度法同时测定饮料中山梨酸钾和苯甲酸钠[J]. 检测与分析, 2006, 8(9): 34~35.
[9] 谭平华, 唐胜伟, 刘长军, 等. 气相色谱法同时测定苯甲醛、苯甲醇和苯甲酸[J]. 天然气化工, 2007(32): 64~66.
[10] Bahruddin Saad, Md Fazlul Bari, Muhammad Idiris Saleh, et al. Simultaneous determination of preservatives (benzoic acid, sorbic acid,methylparaben and propylparaben) in foodstuffs using high-performance liquid chromatography[J]. Journal of Chromatography A, 2005, 1 073(1~2): 393~397.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.