The inhibiting effect of stachyose from Stachys floridana Schuttl.ex Benth on the proliferation of Caco-2 cell line and its potential mechanism were investigated.Caco-2 cells were incubated in serum-free media, the inhibiting effect of stachyose on Caco-2 cell proliferation were analyzed by MTT assay and the apoptosis of Caco-2 cells were determined by flow cytometry, then cell damage degree was studied by the detection of LDH release rate. The results showed that stachyose inhibited the proliferation of Caco-2 cell lines in a dose- and time- dependent manner. After pretreatment with different concentrations of stachyose (0.5, 1, 2, 4 mmol/L), cell apoptosis were obtained. The rise of LDH release rate indicated that stachyose inhibited the reduction ability of pyruvate to lactic acid, and limited the ability of gaining energy via glycolysis path way in Caco-2 cells, which suggests the inhibiting effect of stachyose on the proliferation of tumor cell may be relatedto hindering anaerobic glycolysis pathway and reducing the release of energy.

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[1] 陈燕, 钟先锋, 黄桂东, 等. 银条水苏糖提取条件研究[J]. 天然产物研究与开发, 2011, 23(1):123~130.
[2] Pukacka S, Ratajczak E, Kalemba E. Non-reducing sugar levels in beech (Fagus sylvatica) seeds as related to withstanding desiccation and storage[J]. J. Plant Physiol,2009,166(13):1 381~1 390.
[3] 马丽苹.银条多糖分离纯化、抗肿瘤和免疫调节活性研究[D].南京:南京农业大学,2012.
[4] 宋春丽,王文侠,曾风彩,等.超声波和微波辅助提取大豆低聚糖的工艺比较[J].食品与机械,2011,27(2):47~50.
[5] 岳春,李靖靖,方永远.虫草多糖微波辅助提取工艺的优化[J].食品与机械,2014,30(1):192~195.
[6] Grmanová M, Rada V, Sirotek K, et al. Naturally occurring prebiotic oligosaccharides in poultry feed mixtures[J]. Folia Microbiol (Praha), 2010, 55(4):326~328.
[7] Shin R, Suzuki M, Mizutani T, et al. Improvement of experimentally induced hepatic and renal disorders in rats using lactic acid bacteria-fermented soybean extract (Biofermentics TM)[J]. Evid Based Complement Alternat Med, 2009, 6(3):357~363.
[8] Dan Qiu, Tri Vuong, Babu Valliyodan, et al. Identification and characterization of a stachyose synthase gene controlling reduced stachyose content in soybean[J]. Theor Appl Genet, 2015, 128(11):2 167~2 176.
[9] 魏小龙,茹祥斌,刘福君,等.低分子量地黄多糖对癌基因表达的影响[J].中国药理学与毒理学杂志,1998,12(2):159~160.
[10] 陈超,许莉,王栋.地黄中糖类的变化对其抗癌作用的影响[J].实用肿瘤学杂志,2002,16(4):312.
[11] Rastall R A. Functional oligosaccharides: application and manufacture[J].Annu Rev Food Sci. Technol, 2010(1):305~339.
[12] Zhong Xian-feng, Huang Gui-dong, Chen Yan, et al. Optimization of extracting stachyose from Stachys floridana Schuttl.ex Benth by response surface methodology[J]. J. Food Sci. Technol, 2013, 50(5):942~949.
[13] 马伟伟,李丽,周革非.海黍子硫酸多糖体外免疫与抗肿瘤活性[J].食品科学,2013,34(7):270~274.
[14] 张平,李鹏飞,金素钰,等.蛋白质变性剂和金属离子对中华皮蝇单体乳酸脱氢酶活力的影响[J].湖北农业科学,2014,53(7):1 609~1 612.
[15] 姜绍通,郑志,潘丽军,等.6L-乳酸米根霉发酵体系LDH活力及代谢调控研究[J].食品科学,2005,26(1):41~44.
[16] 杨艳,刘东波,康信聪,等. H2O2诱导胰岛RIN-m5F细胞构建氧化应激模型[J].食品与机械,2014,30(2):40~43.
[17] 侍银宝,钟海雁.油茶果皮多酚粗提物对DNA氧化损伤的影响[J].食品与机械,2013,29(6):143~146.
[18] 亓树艳,王荔,莫晓燕.大枣多糖的提取工艺及抗氧化作用研究[J].食品与机械,2012,28(4):117~120.

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