The seed of Synespalum dulcificum was untrasonified in the acetone-(NH4)2SO4 aqueous two-phase syetem to extract total polyphenils and different conditions for extraction of polyphenols antioxidant activity was investigated. Acetone was chosen as the best extraction solvent for this research. The acetone concentration, amount of the (NH4)2SO4, the ultrasonic time,extraction temperature, ratio of water to solid were discussed by the single factor experiment. The optimal extraction conditions were found to be: the concentration of acetone solution 50%, ultrasonic time 100 min and extraction temperature 60 ℃,ratio of water to solid 20∶1(V∶m). The maximum extraction yield of total polyphenils was 11.54% in the optimum conditions. Under the optimum conditions, through duplicated experiment the yield of polyphenols was 11.56%, it indicated that the extraction rate of polyphenols was extremely stable. The purity of polyphenols extracted with this system is 87.85%, superior to only use ultrasonic, preliminary purification can be realized. The results of the experiment on different conditions for extraction of polyphenols antioxidant activity showed that it has strong ability of removing DPPH free radical and reducing power and to the polyphenol content. The maximum DPPH scavenging rate by polyphenols was up to 62%, higher then that by ascorbic acid.

Publication Date


First Page


Last Page





[1] Ceorge E L, Chen Die-jun. Contents of phenolics and flavonoids and antioxidant activities in skin, pulp, and seeds of miracle fruit[J]. Food Science, 2011, 2(106): 479~482.
[2] 卢圣楼. 神秘果叶营养价值和挥发油化学成分分析及其总黄酮提取纯化与药理化学评价[D]. 海口: 海南师范大学, 2013.
[3] 钟剑珊, 欧阳伟, 袁晓清. 神秘果叶对小鼠高尿酸血症模型影响观察[J]. 亚太传统医药, 2013, 9(8): 9~10.
[4] 游凤, 黄立新, 张彩虹, 等. 超声波辅助减压提取枣皮多酚的研究[J]. 林产化学与工业, 2014, 34(1): 107~112.
[5] Lee L-S, Lee N, Kim Y H, et al. Optimization of ultrasonic extraction of phenolic antioxidants from green tea using response surface methodology[J]. Molecules, 2013, 18: 13 530~13 545.
[6] 魏银花, 申迎宾, 王立. 紫米多酚提取工艺及抗氧化活性研究[J]. 食品与机械, 2013, 29(3): 113~115.
[7] 周晔, 王伟, 陶冉, 等. 超声波提取核桃内种皮多酚的响应面优化及其抗氧化研究[J]. 林产化学与工业, 2013, 33(4): 73~78.
[8] 马立然, 黄亮, 李安平, 等 超声波辅助双水相体系提取橡实仁多酚的研究[J]. 食品工业科技, 2012, 33(3): 191~194.
[9] 马永强, 荆丽荣, 刘晓飞, 等 双水相超声波法辅助提取甜玉米芯多酚及抑菌性研究[J]. 食品科学, 2013, 34(24): 61~64.
[10] Mohsen S M, Ammar A S M. Total phenolic contents and antioxidant activity of corn tassel extracts[J]. Food Chemistry, 2009, 112(3): 595~598.
[11] 卢俊彩, 崔岩岩, 陈火林, 等. 丙酮—硫酸铵双水相体系分离纯化红花红色素[J]. 化学研究与应用, 2007, 19(6): 679~682.
[12] 陈亮, 李医明, 陈凯先, 等. 植物多酚类成分提取分离研究进展[J]. 中草药, 2013, 44(11): 1 501~1 507.
[13] Sabir S M, Ahmad S D, Hamid A, et al. Antioxidant and hepatoprotective activity of enthanolic extract of leaves of Solidago microglossa containing polyphenolic compounds [J]. Food Chemistry, 2012, 131(3): 741~747.
[14] Dorman H J D, Hitunen R. Fe(Ⅲ) reductive and free radical-savenging properties of summer savory (Saturejahortensis L.) extract and subfractions[J]. Food Chemistry, 2004, 88(2): 193~201.
[15] 金寒冰, 方丽, 赖蓓蕾, 等. 苦苣多酚超声辅助提取及抗氧化研究[J]. 食品与机械, 2014, 30(5): 211~215.
[16] Chen Yang, Zhang Hao, Tian Xi, et al. Antioxidant potential of crocins and ethanol extracts of Gardenia jasminoides ELLIS and Crocus sativua L: A relationship investigation between antioxidant activity and crocin contents[J]. Food Chemistry, 2008, 109(3): 484~492.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.