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Abstract

The specific LAMP primers of genetically modified maize NK603 were designed on the basis of the published sequence (GenBank accession number AX342369). Established the specific loop-mediated isothermal amplification (LAMP) method for the detection of the transgenic maize line NK603 strains. The specificity and sensitivity of this method were evaluated. The results showed that LAMP could detect the transgenic maize NK603 from several GM events specifically and effectively with LOD of 0.1%. Since this method is rapid, simple, it could be applied in GMO detection.

Publication Date

1-28-2016

First Page

56

Last Page

58,80

DOI

10.13652/j.issn.1003-5788.2016.01.013

References

[1] 宋君, 雷绍荣, 刘勇, 等. 转基因玉米NK603品系特异定量PCR检测方法的建立[J]. 生物技术通讯, 2012, 23(2): 238-241.
[2] Matsuoka T, Kuribara H, Akiyama H, et al. A multiplex PCR method of detecting combinant DNAs from five lines of genetically modified maize[J]. Shokuhin Eiseigaku Zasshi, 2001, 42(1): 24-32.
[3] Chaouachi M, El Malki R, Berard A, et al. Development of a real-time PCR method for the differential detection and quantification of four solanaceae in GMO analysis: potato (Solanum tuberosum), tomato (Solanum lycopersicum), eggplant (Solanum melongena), and pepper (Capsicum annuum)[J]. Journal of Agricultural and Food Chemistry, 2008, 56(6): 1 818-1 828.
[4] Notomi T, Okayama H, Masubuchi H, et al. Loop-mediated isothermal amplification of DNA[J]. Nucleic Acids Res, 2000, 28(12): e63.
[5] Misawa Y, Yoshida A, Saito R, et al. Application of loop-mediated isothermal amplification technique to rapid and direct detection of methicillin-resistant Staphylococcus aureus (MRSA) in blood cultures[J]. J. Infect Chemother, 2007, 13(3): 134-140.
[6] 张淑红, 吴清平, 徐晓可, 等. LAMP法在阪崎肠杆菌快速检测中的应用[J]. 食品与机械, 2011, 27(5): 111-114.
[7] Chen Qin, Li Jian, Deng Zhi-rui, et al. Comprehensive detection and identification of seven animal coronaviruses and human respiratory coronavirus 229E with a microarray hybridization assay[J]. Intervirology, 2010, 53(2): 95-104.
[8] Han Eun-taek, Watanabe R, Sattabongkot J, et al. Detection of four plasmodium species by genus- and species-specific loop-mediated isothermal amplification for clinical diagnosis[J]. J. Clin. Microbiol., 2007, 45(8): 2 521-2 528.
[9] 刘昊, 黄文胜, 邓婷婷, 等. LAMP法检测食品中开心果过敏原成分[J]. 食品科学, 2013, 34(22): 128-132.
[10] 张舒亚, 李富威, 于翠, 等. 环介导等温扩增法检测食品过敏原大豆成分[J]. 食品安全质量检测学报, 2013, 4(2): 451-456.
[11] 李一鸣, 王宇珂, 叶宇鑫, 等. 环介导等温扩增技术检测花生过敏原[J]. 现代食品科技, 2012, 28(1): 126-130.
[12] Vaagt F, Haase I, Fischer M. Loop-mediated isothermal amplification (LAMP)-based method for rapid mushroom species identification[J]. J. Agric. Food Chem., 2013, 61(8): 1 833-1 840.
[13] Focke F, Haase I, Fischer M. Loop-mediated isothermal amplification (LAMP): Methods for plant species identification in food[J]. J. Agric. Food Chem., 2013, 61(12): 2 943-2 949.
[14] 杨丽霞, 付淑君, 彭新凯. 环介导等温扩增法检测牛羊肉中的猪肉成分[J]. 食品与机械, 2013, 29(5): 63-65.
[15] Guan Xiao-yan, Guo Jin-chao, Shen Ping, et al. Visual and rapid detection of two genetically modified soybean events using loop-mediated isothermal amplification method[J]. Food Anal Methods, 2010, 3(4): 313-320.
[16] Rostamkhani N, Haghnazari A, Tohidfar M, et al. Rapid identification of transgenic cotton (Gossypium hirsutum L.) plants by loop-mediated isothermal amplification[J]. Czech J. Genet Plant Breed, 2011, 47(4): 140-148.
[17] Chen Li-li, Guo Jin-chao, Wang Qi-di, et al. Development of the visual LAMP assays for seven genetically modified maize events and their application in practical samples analysis[J]. J. Agric. Food Chem., 2011, 59(11): 5 914-5 918.
[18] Chen Xiao-yun, Wang Xiao-fu, Jin Nuo, et al. Endpoint visual detection of three genetically modified rice events by loop-mediated isothermal amplification[J]. Int. J. Mol. Sci., 2012, 13(11): 14 421-14 433.
[19] 吴少云, 唐大运, 李琳, 等. LAMP实时浊度法检测转基因水稻Bt63品系[J]. 食品与机械, 2012, 28(5): 79-82.
[20] Onishi M, Matsuoka T, Kodama T, et al. Development of a multiplex polymerase chain reaction method for simultaneous detection of eight events of genetically modified maize[J]. J. Agric. Food Chem., 2005, 53(25): 9 713-9 721.

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