The removal efficiency of electrolyzed oxidizing water on biofilm of Staphylococcus aureus was studied. Electrolyzed oxidizing water showed obvious damage on the Staphylococcus aureus biofilm,including substantially destroyed external matrix, severely destroyed cells and decreased bacterial number observed with SEM(scanning electron microscope)on 5 000-times and 40 000-times. The removal efficiency could be reduced significantly by prolonging storage time of oxidation potential acidic water , raising preservation temperature and the presence of organic interfering substances. The removal efficiency of sealing preservation was superior to openning preservation. The available chlorine, pH value and redox potential had a synergistic effect on the removal efficiency. When the amount of available chlorine was low, the removal efficiency of concentration variation was significant. When the amount of chlorine was high, the removal efficiency of concentration variation was not significant. Under low pH value conditions, the removal efficiency was comparatively better. The redox potential has a significant positive correlation with the removal efficiency.

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[1] Pitts B. A microtiter screening method for biofilm disinfection and removal[J]. Journal of Microbiological Methods, 2003, 54(2): 269-276.
[2] Hall-Stoodley L, Costerton J W, Stoodley P. Bacterial biofilms: from the natural environment to infectious diseases[J]. Nature Reviews in Microbiology, 2004(2): 95-108.
[3] Cyril J S, Davida S S, Jean K, et al. Staphylococcus aureus: From man or animals-an enterotoxin iceberg[C]//Proceeding of an international EU-RAIN conference hosted by the istituto Zooprofilattico Sperimentale delle Venezie, Padua, Italy, December 2nd-3nd, Italy: The National Food Center, 2004: 85-102.
[4] Izano E A, Amarante M A, Kher W B, et al. Differential roles of poly-N-acetylglucosamine surface polysaccharide and extracellular DNA in Staphylococcus aureus and Staphylococcus epidermidis biofilms[J]. Appl. Environ. Microbiol., 2008, 74(2): 470-476.
[5] Gibson H, Taylorl J H, Hall K E, et al. Effectiveness of cleaning techniques used in the food industry in terms of the removal of bacterial biofilms[J]. Appl. Microbiol., 1999, 87(1): 41-48.
[6] Tot K, Horemans T, Vander Berghe D, et al. Inhibitory effect of biocides on viable mass and matrix of Staphylococcus aureus and Pseudomonas aeruginosa biofilms[J]. Environ. Microbiol., 2010, 76(10): 3 135-3 142.
[7] Van Der Plas M, Dambrot C, Dambrot C, et al. Combinations of maggot excretions/secretions and antibiotics are effective against Staphylococcus aureus biofilms and the bacteria derived therefrom[J]. Antimicrob Chemother, 2010, 65(5): 917-923.
[8] 宗绪岩, 李丽, 罗惠波, 等. 啤酒糟蛋白水解物对金黄色葡萄球菌抑菌能力研究[J]. 食品与机械, 2012, 28(1): 108-111.
[9] Harrison J J, Turner R J, Jooda, et al. Copper and quaternary ammonium cations exert synergistic bactericidal and antibiofilm activity against Pseudomonas aeruginosa[J]. Antimicrob Agents Chemother, 2008, 52(8): 2 870-2 881.
[10] Park C M, Hung Y C, Chun G D. Effects of chlorine and pH on efficacy of electrolyzed water for inactivatingEscherichia coli O157:H7 and Listeria monocytogenes[J]. International Journal of Food Microbiology, 2004, 91(1): 13-18.
[11] Huang Yu-ru, Hung Yen-con, Hsu Shun-yao, et al. Application of electrolyzed water in the food industry[J]. Food Control, 2008, 19(4): 329-345.
[12] 贾兴真, 银燕, 张聿为. 一种酸性氧化电位水理化性能及杀菌效果研究[J]. 中国消毒学杂志, 2015, 32(5): 440-442.
[13] 卫生部卫生法制与监督司. 消毒技术规范[S]. 北京: 中华人民共和国卫生部, 2008.
[14] Heilmann C, Gerke C, Perdreau-Remington F, et al. Characterization of Tn917 insertion mutants of Staphylococcus epidermidis affected in biofilm formation[J]. Immun, 1996, 64(1): 277-282.
[15] 李燕杰, 朱小花, 阴冠秀, 等. 不同方法观察单增李斯特菌生物被膜的比较研究[J]. 食品工业科技, 2010(10): 241-246.
[16] 牛晓雯, 常晓玲. 漂液中有效氯含量检测方法的改进[J]. 黑龙江造纸, 2007(1): 58-60.
[17] Kim C, Hung Y C, Brachett R E. Roles of oxidation-reduction potential in electrolyzed oxidizing and chemically modified water for inactivation of food-related pathogens[J]. Journal of Food Protection, 2000, 63(1): 19-24.
[18] 任占冬, 朱玉婵, 刘晔, 等. 肉糜的电位水杀菌工艺与机理[J]. 农业机械学报, 2009(12): 139-143.
[19] Liao Long B, Chen Wei M, Xiao Xian M. The generation and inactivation mechanism of oxidation reduction potential of electrolyzed oxidizing water[J]. Journal of Food Engineering, 2007, 78(4): 1 326-1 332.
[20] 侯梦石, 曹薇, 赵淑梅, 等. 电解水对叶菜杀菌效果的研究[J]. 北方园艺, 2010(24): 46-47.



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