Abstract
The objectives of the study were to screen the strain with a good antioxidation activity from 10 species of wild macrofungi and optimize the optimal conditions for antioxidant substance production. These results can provide some references for the breeding of Auricuiaria auricular and industrialization of its production. As material with 10 strains of wild fungi from the Langyashan in Anhui province, the strains with antioxidant substance producing were obtained by DPPH free radical scavenging rate of the broth. Shake flask culture was then used to get the optimum culture conditions based on the single factor and orthogonal experiments, which is finally examined. The results showed that there were four strains with producing antioxidation activity in the samples, accounting for 40%. The strain of Auricuiaria auricular has especially the higher capacity of antioxidaton activity. The formula of appropriated culture conditions were as follows: glucose 25 g/L, yeast extract 2 g/L, MgSO4 0.2 g/L, CuSO4 6 mg/L, K2HPO4 3.2 g/L, pH 6.0, 27 ℃, culture volume 100 mL/250 mL, and cultivation 8 d. The metabolites of the Auricuiaria auricular have good antioxidant activity, providing a new source of natural antioxidants development.
Publication Date
1-28-2016
First Page
125
Last Page
129
DOI
10.13652/j.issn.1003-5788.2016.01.031
Recommended Citation
Xinyi, CHAI; Shijun, YU; Weiwei, ZHANG; and Zehu, DU
(2016)
"Screening of strain with antioxidation activity from wild macrofungi and optimization on its fermentation conditions,"
Food and Machinery: Vol. 32:
Iss.
1, Article 31.
DOI: 10.13652/j.issn.1003-5788.2016.01.031
Available at:
https://www.ifoodmm.cn/journal/vol32/iss1/31
References
[1] Halliwell B, Murcia M A, Chirico S, et al. Free radicals and antioxidants in food and in vivo: What they do and how they work[J]. Critical Reviews in Food Science and Nutrition, 1995, 35(1): 7-20.
[2] Volpi N, Tarugi P. Influence of chondroitin sulfate charge density, sulfate group position, and molecular mass on Cu2+ mediated oxidation of human low-density lipoproteins: effect of normal human plasma derived chondroitin sulfate[J]. Journal of Biochemistry, 1999, 125(2): 297-304.
[3] 贾春风, 刘松雁, 陈梅香, 等. 紫薯发酵醋工艺优化及其抑菌活性研究[J]. 食品与机械, 2014, 30(4): 211-215.
[4] 刘力, 李理. 酸浆水中3种菌株的产酸能力及抗氧化活性[J]. 食品与机械, 2015, 31(2): 11-15.
[5] 曾伟, 丁利君, 黄聪华, 等. 黑曲霉发酵法辅助提取芒萁黄酮及其抗氧化研究[J]. 食品与机械, 2012, 28(3): 115-118.
[6] 马玲.3 种食用菌中多糖的提取与测定[J]. 中国卫生检验杂志, 2005, 15(2): 174.
[7] Lindequist U, Niedermeyer T, Julich W D. The pharmacological potential of mushrooms[J]. Evidence-Based Complementary and Alternative Medicine, 2005, 2(3): 285-299.
[8] 陈康林, 卯晓岚, 黄明达. 中国抗肿瘤大型药用真菌图鉴[M]. 北京: 科学出版社, 2013: 1-10.
[9] Lee I K, Kim S E, Yeom J H, et al. Daldinan A, a novel isoindolinone antioxidant from the ascomycete Daldinia concentrica[J]. The Journal of Antibiotics, 2012, 65(2): 95-97.
[10] Bai Ming-sheng, Wang Chen, Zong Shi-chun, et al. Antioxidant polyketide phenolic metabolites from the edible mushroom Cortinarius purpurascens[J]. Food Chemistry, 2013, 141(4): 3 424-3 427.
[11] Liu Kun, Wang Jun-li, Zhao Le, et al. Anticancer, antioxidant and antibiotic activities of mushroom Ramaria flava[J]. Food and Chemical Toxicology, 2013, 58(8): 375-380.
[12] Lee I K, Jung J Y, Kim Y H, et al. Phellinins B and C, new styrylpyrones from the culture broth of Phellinus sp.[J]. The Journal of Antibiotics, 2010, 63(5): 263-266.
[13] 李峻志, 吴小杰, 党永, 等. 大型真菌抗氧化活性物质的研究进展[J]. 食品与生物技术学报, 2014, 33(5): 455-465.
[14] 张北红, 宋瑞清, 刘淑双, 等. 高效抗氧化大型真菌的筛选[J]. 菌物研究, 2010, 8(4): 213-218.
[15] 杨建, 袁海生, 曹云. 5种野生多孔菌提取物的抗氧化活性[J]. 食品科学, 2011, 32(13): 40-44.
[16] Tina S, Chi T H. Antioxidant activities of buckwheat extracts[J]. Food Chemistry, 2005, 90(4): 743-749.
[17] 葛飞, 石贝杰, 高樱萍, 等. 一株高抗氧化活性银杏内生真菌SG0016的鉴定及其培养条件优化[J]. 西北植物学报, 2015, 35(2): 403-409.