The experiment used defatted rice bran as materials which extract water-soluble polysaccharide from it, expecting to improve its functional activities by modification. The carboxymethylated derivative of polysaccharide was prepared by the reaction between rice bran polysaccharide and chloroacetic acid (MCA) in alkaline condition and fourier infrared spectrum suggested that carboxymethylated rice bran polysaccharide was successfully carboxymethylated without changing the original structure of rice bran polysaccharide. The optimal technological conditions for the reaction are: reaction temperature 52 ℃, reaction time 2.5 h, concentration of NaOH 1.2 mol/L, MCA dose 3.20 g. This condition of the rice bran polysaccharide carboxymethylated substitution degree(DS) resulted to a maximum value of 0.99.

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[1] 曹蕊. 米糠营养成分分析及其应用研究[D]. 无锡: 江南大学, 2008: 1-3.
[2] 韩素云, 刘磊, 张名位, 等. 米糠整体加工利用研究进展[J]. 广东农业科学, 2014(12): 89-94.
[3] Min B, McClung A M, Chen M H. Phytochemicals and antioxidant capacities in rice brans of different color[J]. J. Food Sci., 2011, 76(1): C117-C126.
[4] Garc E R M, Guerra H E, Garc V B. Phytic acid content in milled cereal products and breads[J]. Food Research International, 1999, 32(3): 217-221.
[5] 王旭, 王鹏, 王娜, 等. 米糠营养速溶粉工艺优化[J]. 食品与机械, 2014, 30(5): 247-249.
[6] 胡国华, 杨帆, 马正智, 等. 米糠多糖的研究及应用进展[J]. 中国食品添加剂, 2007(5): 80-82.
[7] Wang Li, Zhang Hong-bin, Zhang Xiao-yan, et al. Purification and identification of a novel heteropolysaccharide RBPS2a with anti-complementary activity from defatted rice bran[J]. Food Chemistry, 2008, 110(1): 150-155.
[8] Li Shu-fang, Yang Han-chun, Zhang Ji-dong, et al. Effects of rice bran polysaccharide on peripheral T lymphocyte proliferation activation and serum ND antibody titer after vaccination in immunosuppression chickens induced by cyclophosphamide[J]. Veterinary Science in China, 2007, 37(7): 588-592.
[9] 王莉. 米糠多糖及其硫酸酯的结构、抗肿瘤活性的研究[D]. 无锡: 江南大学, 2009: 58-63.
[10] 孙月娥, 王卫东, 王陶. 菊糖硫酸化改性研究[J]. 食品与发酵工业, 2011, 37(11): 95-98.
[11] 王争昱, 贾小燕, 柴保臣, 等. 菊粉提取及羧甲基菊粉钠的制备[J]. 郑州大学学报, 2007, 44(1): 192-194.
[12] Verraest D L, Peters J A, Kuzee H C, et al. Distribution of substituents in O-carboxymethyl and O-cyanoethyl ethers of inulin[J]. Carbohydrate Research, 1997, 30(2): 203-212.
[13] 焦中高. 红枣多糖的分子修饰与生物活性研究[D]. 陕西: 西北农林科技大学, 2012: 112-115.
[14] 李杰, 罗志刚, 肖志刚, 等. 挤压超声联用提取米糠多糖工艺优化[J]. 农业机械学报, 2013, 44(3): 174-179.
[15] 刘光宪, 冯健雄, 王辉, 等. 不同纯化方法对花生多糖抗氧化活性的影响[J]. 食品与机械, 2014, 30(1): 189-190.
[16] Durcilece A S, Regina C M, Judith P A, et al. Carboxymethylation of cashew tree exudate polysaccharide[J]. Carbohydrate Polymers, 2004, 58(2): 163-171.
[17] Eyler R W, Klug E D, Diephuis F. Determination of degree of substitution of sodium carboxymethylcellulose[J]. Analytical Chemistry, 1947, 19(1): 24-27.
[18] 焦中高, 刘杰超, 王思新, 等. 羧甲基化红枣多糖制备及其活性[J]. 食品科学, 2011, 32(17): 176-180.
[19] Fan Jun-min, Zhang Jing-song, Tang Qing-jiu. Structural elucidation of a neutral fucogalactan from the mycelium of Coprinuscomatus[J]. Carbohydrate Research, 2006, 341(9): 1 130-1 134.
[20] Verraest D L, Peters J A, Kuzee H C, et al. Carboxymethylation of inulin[J]. Carbohydrate Research, 1995, 271(1): 101-112.
[21] Xu Jing, Liu Wei, Yao Wen-bing, et al. Carboxymethlation of a polysaccharide extracted from Ganoderma lucidum enhances ist antioxidant activities in vitro[J]. Carbohydrate Polymers, 2009, 78(2): 227-234.



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