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Abstract

Vaccinium Bracteatum Thunb Leaves (VBTL) was used as material. An efficient ultrasound/microwave assisted extraction (UMAE) technique was employed to extract flavones from VBTL. The effects of microwave power, ratio of solvent to solid, temperature and time on the yield of flavones from VBTL were investigated. To optimize the extraction process, response surface methodology was used based on the results of single factor experiment. IR spectrum was scanned to validate the effect of UMAE technology compared with the traditional solvent extraction technology. The results indicated the optimal conditions of UMAE were 140 W of microwave power, 50 W of ultrasound power, 69 ℃ of temperature, 51∶1(V∶m) of solvent-solid ratio and 11 min of extraction time, respectively. The yield of flavones was 3.64% under the optimal technology. The results of IR spectrum implied UMAE had no influence on the structure of flavones from VBTL.

Publication Date

1-28-2016

First Page

148

Last Page

153

DOI

10.13652/j.issn.1003-5788.2016.01.036

References

[1] 蔡凌云.乌饭树叶中总黄酮体外抗氧化活性[J].中成药,2011,33(6):1 054-1 057.
[2] Daniela B, Luisa T, Francesca D G, et al. Antioxidant activities of sicilian prickly pear(opuntia ficus indica)fruit extracts and reducing properties of its betalains:betanin and indicaxanthin[J]. Agricultural and Food Chemistry, 2002, 50(23): 6 895-6 901.
[3] 曾伟,丁利君,黄聪华,等.黑曲霉发酵法辅助提取芒萁黄酮及其抗氧化研究[J].食品与机械,2012, 28(3): 115-118.
[4] 章海燕,王立,张晖.乌饭树树叶水溶性黄酮的抑菌作用的研究[J].中国食品添加剂,2010(5): 62-67.
[5] Edziri H, Mastouri M, Ammar S, et al. Antimicrobial, antioxidant, and antiviral activities of retama raetam(forssk.) webb flowers growing in Tunisia[J]. World Journal of Microbiology and Biotechnology, 2008, 24(12): 2 933-2 940.
[6] Gomah N. Antimicrobial activity of Calotropis procera Ait.(Asclepiadaceae)and isolation of four flavonoid glycosides as the active constituents[J]. World Journal of Microbiology and Biotechnology,2013(29): 1 255-1 262.
[7] 黄丽娜,马文领,周健,等.乌饭树叶醇提物抗大鼠精神疲劳作用[J].中国公共卫生,2008, 24(8): 964-966.
[8] Wang Li, Zhang Xue-tong, Zhang Hai-yan, et al. Effect of Vaccinium bracteatum Thunb. Leaves extract on blood glucose and plasma lipid levels in streptozotocin-induced diabetic mice[J]. Journal of Ethnopharmacology, 2010, 130(3): 465-469.
[9] 章海燕,王立,张晖.基于响应面分析法优化的乌饭树树叶水溶性黄酮提取条件[J].食品工业科技,2010, 31(3): 260-263.
[10] 余青,许慧星,肖小蓉,等.基于响应面分析法优化的乌饭树叶总黄酮提取条件[J].中国农学通报,2008, 24(1): 93-98.
[11] 王立,姚惠源.乌饭树树叶中黄酮类色素的提取与分离纯化[J].食品与发酵工业,2004, 30(9): 120-125.
[12] 余青,郑小严,黄红霞,等.超临界CO2萃取乌饭树叶总黄酮的工艺[J].福建农林大学学报,2009, 38(1): 97-101.
[13] Zhao Gui-li, Chen Xue, Wang Lei, et al. Ultrasound assisted extraction of carbohydrates from microalgae as feedstock for yeast fermentation[J]. Bioresource Technology, 2013, 128(1): 337-344.
[14] 陈丛瑾,黄克瀛,李德良,等.香椿叶总黄酮的超声波辅助提取及其清除DPPH自由基能力的研究[J].食品与机械,2007, 23(1): 76-80.
[15] 王娟,沈平孃,沈永嘉.葛根中有效成分微波辅助萃取研究[J].中国医药工业杂志,2002, 33(8): 382-384.
[16] 苏东林,单杨,李高阳.微波法提取柑桔皮总黄酮的应用研究[J].食品与机械,2007, 23(3): 73-77.
[17] 刘全德,唐仕荣,宋慧.超声波—微波协同萃取芹菜黄酮的工艺研究[J].食品与机械,2010, 26(5): 134-137.
[18] Ranjan A, Patil C, Moholkar V S. Mechanistic assessment of microalgal lipid extraction[J]. Industrial Engineering Chemistry Research, 2010(49): 2 979-2 985.
[19] Ahmad-Qasem M H, Cánovas J, Barrajón-Catalán E, et al. Kinetic and compositional study of phenolic extraction from olive leaves (var. Serrana) by using power ultrasound[J]. Innovative Food Science Emerging Technologies, 2013(17): 120-129.
[20] 唐浩国.黄酮类化合物研究[M].香港:科学教育出版社,2009: 42-43.
[21] 黄佳萍,郑青,郭梦梦,等.超声波法提取乌饭树叶槲皮素的工艺研究[J].安徽农业科学,2013, 41(9): 4 050-4 052.
[22] 顾文秀,谢为明,夏文水,等.超声波提取乌饭树叶色素及其稳定性的研究[J].林产化学与工业,2005, 25(4): 74-78.
[23] 胡志杰,蔡智慧.微波辅射提取乌饭树叶黑色素工艺的研究[J].中国野生植物资源,2007, 26(6): 52-54.
[24] 王立.乌饭树黑色素中功能性成分研究[D].无锡:江南大学,2005.
[25] 王芳,张庆庆,乔璐,等.乌饭叶中黄酮、多酚的提取及其抗氧化性研究[J].湖北农业科学,2014, 53(17): 4 158-4 163.

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