Studies on volatile oil extracting process optimization by supercritical CO₂ and analysis by GC—MS of Allium mongolicum Regel flowers

Authors

WEN Junfeng, Yulin University, Yulin, Shaanxi 719000, China; Yulin Key Laboratory of Deep Processing of Agricultural products, Yulin, Shaanxi 719000, China
LIU Xia, Yulin University, Yulin, Shaanxi 719000, China
GAO Liguo, Yulin University, Yulin, Shaanxi 719000, China; Yulin Key Laboratory of Deep Processing of Agricultural products, Yulin, Shaanxi 719000, China
LI Rui, Yulin University, Yulin, Shaanxi 719000, China

Abstract

The volatile oil from the Allium mongolicum Regel flowers by supercritical-CO₂ fluid extraction(SFE) was optimized using response surface methodology(RSM)，and the volatile oil was identified by GC—MS. The effects of the extraction pressure, extraction temperature, extraction time on the yield of volatile oil were investigated by a three-factor and three-level Box-Behnken central composite design with the extraction rate as the response value. The results showed that the optimum conditions for the extraction of Allium mongolicum Regel flower oil were as follows: extracting temperature 39 ℃, pressure 29 MPa, time 1.2 h, CO₂ flux 20 L/ h. Under such condition, the model-predicted and experimental values of oil yield were 19.36% and 18.90%. The 29 chemical components were separated by GC—MS, which were 96.67% of the total oil, and 27 compounds of them were also identified, including 8 aldehydes(37.63%), aromatic hydrocarbon compounds(30.52%) and Gingerol (12.60%).

Publication Date

11-28-2016

First Page

158

Last Page

162

DOI

10.13652/j.issn.1003-5788.2016.11.036

Recommended Citation

Junfeng, WEN; Xia, LIU; Liguo, GAO; and Rui, LI (2016) "Studies on volatile oil extracting process optimization by supercritical CO₂ and analysis by GC—MS of Allium mongolicum Regel flowers," Food and Machinery: Vol. 32: Iss. 11, Article 36.
DOI: 10.13652/j.issn.1003-5788.2016.11.036
Available at: https://www.ifoodmm.cn/journal/vol32/iss11/36

References

[1] 贺访印, 刘世增, 严子柱, 等. 野生沙葱的资源分布与保护利用[J]. 中国野生植物资源, 2007, 26(2): 14-17.
[2] 巴俊杰, 张春丽, 高建萍, 等. 沙葱营养成分分析[J]. 内蒙古农业大学学报, 2002, 23(4): 114-115.
[3] 乌仁张嘎, 张兴夫, 敖长金. 沙葱挥发油提取工艺的研究[J]. 饲料工业, 2011, 32(7): 37-40.
[4] 张婷, 杨波, 罗瑞明, 等. 响应面法优化沙葱的自然发酵工艺[J]. 中国酿造, 2014, 33(10): 94-98.
[5] 黄赛金, 尹爱武, 罗紫英, 等. 高良姜挥发油抑菌及抗氧化作用研究[J]. 食品工业科技, 2015, 36(19): 112-115.
[6] 耿东升, 王雪飞, 陈雪莲. 瘤果黑种草子挥发油抗炎、祛痰药理作用研究[J]. 中国医院药学杂志, 2014, 34(8): 605-607.
[7] 卫强, 鲁轮, 龙先顺, 等. 提取方法对木槿叶挥发油成分及其对肺癌A549细胞抑制作用的影响[J]. 食品与机械, 2016, 32(4): 160-166.
[8] 何月秋, 林立, 杜甜钿, 等. 紫娇花挥发油成分的GC-MS分析及抗氧化能力测定 [J/OL]. 广西植物, http://www.cnki.net/kcms/detail/45.1134.Q.20160328.1534.008.html.
[9] 刘超祥, 丁锐, 王珊, 等. 亳州疏毛罗勒挥发油化学成分及抗氧化与抑菌活性研究[J]. 长江大学学报: 自科版, 2016, 13(21): 34-37.
[10] 刘世巍, 赵堂, 杨敏丽. GC-MS分析沙葱挥发油的化学成分[J]. 华西药学杂志, 2007, 22(3): 313-314.
[11] 易雪静, 刘刚, 龚铮午. 微波联合纤维素酶提取艾叶挥发油的研究[J]. 食品与机械, 2016, 32(3): 160-164.
[12] 李超, 杨日福, 傅雪峰, 等. 超声强化超临界CO₂萃取复方丁香肉桂挥发油[J]. 华南理工大学学报: 自然科学版, 2008, 36(7): 67-68.
[13] 李茂迁, 胡萍萍, 王艳艳. Design-Expert 在超临界CO₂萃取工艺优化中的应用[J]. 广东化工, 2011, 38(8): 8-9.
[14] 欧阳辉, 余佶, 陈功锡, 等. 超临界CO₂萃取花椒籽油工艺的响应面优化[J]. 中国油脂, 2009, 34(10): 9-12.
[15] 何花, 雷鸣, 薛金彪, 等. 超临界CO₂萃取菊叶香藜精油工艺研究[J]. 山西农业科学, 2016, 44(2): 253-254.
[16] 李松吴, 光斌, 陈发河. 超临界萃取琯溪蜜柚精油工艺优化及组分分析[J]. 食品与机械, 2013, 29(1): 113-117.
[17] 蒋燕, 蒲彪, 王汉清, 等. 不同方法提取冷榨藤椒油中的油树脂及其成分分析[J]. 食品与机械, 2016, 32(1): 131-134.
[18] 谢志刚, 唐德容, 李乐. 姜辣素的提制及抗氧化性能的研究[J]. 食品与机械, 2006, 22(5): 52-54.
[19] 郁红礼, 毛善虎, 赵腾斐, 等. 姜辣素拮抗半夏凝集素蛋白刺激巨噬细胞所致炎症因子TNF-α释放增加、ROS过量生成及RIP3表达增高[J]. 中国中药杂志, 2015, 40(18): 3 630-3 635.